Journal Article10.1002/jbm.a.37363
An additive manufacturing-based 3D printed poly ɛ-caprolactone/alginate sulfate/extracellular matrix construct for nasal cartilage regeneration.
Pariya Zare,Mohamad Pezeshki-Modaress,Seyed Mohammad Davachi,Hadi Chahsetareh,Sara Simorgh,Negin Asgari,Mohammad Amin Haramshahi,Rafieh Alizadeh,Zohreh Bagher,Mohamad Farhadi +9 more
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TL;DR: Results demonstrated that the scaffold showed mechanical similarity to the native nasal cartilage tissue along with possessing appropriate biochemical features, which makes this new formulation based on PCL/dECM/Alg:Alg-Sul a promising candidate for further in-vivo studies.
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Abstract: Various composite scaffolds with different fabrication techniques have been applied in cartilage tissue engineering. In this study, poly ɛ-caprolactone (PCL) was printed by fused deposition modeling method, and the prepared scaffold was filled with Alginate (Alg): Alginate-Sulfate (Alg-Sul) hydrogel to provide a better biomimetic environment and emulate the structure of glycosaminoglycans properly. Furthermore, to enhance chondrogenesis, different concentrations of decellularized extracellular matrix (dECM) were added to the hydrogel. For cellular analyses, the adipose-derived mesenchymal stem cells were seeded on the hydrogel and the results of MTT assay, live/dead staining, and SEM images revealed that the scaffold with 1% dECM had better viscosity, cell viability, and proliferation. The study was conducted on the optimized scaffold (1% dECM) to determine mechanical characteristics, chondrogenic differentiation, and results demonstrated that the scaffold showed mechanical similarity to the native nasal cartilage tissue along with possessing appropriate biochemical features, which makes this new formulation based on PCL/dECM/Alg:Alg-Sul a promising candidate for further in-vivo studies.
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Citations
Recent advances in 3D-printed polylactide and polycaprolactone-based biomaterials for tissue engineering applications.
Zia Ullah Arif,Muhammad Yasir Khalid,Reza Noroozi,Ali Sadeghianmaryan,Meisam Jalalvand,Mokarram Hossain +5 more
TL;DR: In this paper , the recent advancements in the PLA and PCL biodegradable polymer-based composites as well as their reinforcement with hydrogels and bio-ceramics scaffolds manufactured through 3DP are systematically summarized and the applications of bone, cardiac, neural, vascularized and skin tissue regeneration are thoroughly elucidated.
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Decellularized ECM hydrogels: prior use considerations, applications, and opportunities in tissue engineering and biofabrication.
Jacqueline Kort-Mascort,Salvador Flores-Torres,Omar Peza-Chavez,Joyce H. Jang,L. Pardo,Simon D. Tran,Joseph M. Kinsella +6 more
TL;DR: In this article , the physical and bioactive characteristics of extracellular matrix (ECM) hydrogels and their role as biomaterials for biofabrication are discussed.
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3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties
Zahra Bashiri,Motahareh Rajabi Fomeshi,Hatef Ghasemi Hamidabadi,Davod Jafari,Sanaz Alizadeh,Maryam Nazm Bojnordi,Gorka Orive,Alireza Dolatshahi-Pirouz,Maria Zahiri,Rui L. Reis,Subhas C. Kundu,Mazaher Gholipourmalekabadi +11 more
TL;DR: In this article , the authors used human placental extracellular matrix (ECM) derived from the placenta, sodium-alginate and gelatin to prepare a printable bioink biomimicking natural skin.
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Alginate sulfate/ECM composite hydrogel containing electrospun nanofiber with encapsulated human adipose-derived stem cells for cartilage tissue engineering.
Roghayeh Najafi,Hadi Chahsetareh,Mohamad Pezeshki-Modaress,Mina Aleemardani,Sara Simorgh,Seyed Mohammad Davachi,Rafieh Alizadeh,Alimohamad Asghari,Sajad Hassanzadeh,Zohreh Bagher +9 more
TL;DR: In this paper , a composite hydrogel scaffolds were developed to evaluate the relevant factors and conditions in mechanical properties, cell proliferation, and differentiation to enhance cartilage regeneration, and the results showed promising features for cartilage tissue engineering application.
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Chitosan-placental ECM composite thermos-responsive hydrogel as a biomimetic wound dressing with angiogenic property.
Abdolnaser Azadbakht,Sanaz Alizadeh,Zahra Aliakbar Ahovan,Zahra Khosrowpour,M Majidi,S R Pakzad,Shahrokh Shojaei,Narendra Pal Singh Chauhan,Marzieh Jafari,Mazaher Gholipourmalekabadi +9 more
TL;DR: In this paper , a thermos-responsive hydrogel is fabricated using chitosan (CTS) with various concentrations (1, 2.5, and 5% w/v) of solubilized placental extracellular matrix (ECM) and 20% β-glycerophosphate to optimize a smart wound dressing hydrogels with improved biological behavior.
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