Journal Article10.1016/0003-2697(88)90512-X
Amino acid analysis utilizing phenylisothiocyanate derivatives.
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TL;DR: Using phenylisothiocyanate as the reagent, detection limits under 1 pmol can be routinely achieved, allowing the analysis of submicrogram protein samples, giving the analyst the first realistic alternative to ion-exchange analysis without compromising desirable features of the traditional methodology.
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About: This article is published in Analytical Biochemistry. The article was published on 01 Oct 1988.
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Citations
Metabolic Profiling of Glucose-Fed Metabolically Active Resting Zymomonas mobilis Strains
TL;DR: Detailed patterns of adaptation of central metabolite pools due to the change in cultivation condition and between the mutants and Zm6 reference strain were observed, and decreased NADH/NAD ratio under aerobic incubation corresponded to higher concentrations of the phosphorylated glycolytic intermediates, in accordance with predictions of the kinetic model of Entner–Doudoroff pathway.
11
Fluorescent N-methylanthranilyl (Mantyl) tag for peptides: its application in subpicomole determination of kinins.
TL;DR: Fluorescence detection of Mantyl kinins was about 50-100 times more sensitive than UV detection of the phenylisothiocyanate-derivatized kinins under typical HPLC conditions.
11
Chapter 13 Amino acids and peptides
TL;DR: PITC, also known as Edman's reagent, has long been used for the sequencing of polypeptides and proteins, and was introduced for the analysis of amino acids in the early eighties and is currently the most extensively used reagent for pre-column derivatization.
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Rapid determination of glutamine in biological samples by high-performance liquid chromatography
TL;DR: A high-performance liquid chromatographic method can be used for the determination of glutamine in plasma, urine, cerebrospinal fluid and tissue homogenates and no interference from other amino acids was observed.
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Cosmetic and amino acid analysis of the effects of lye and no-lye relaxer treatment on adult black female South African hair
TL;DR: Hair amino acid analysis by reversed-phase high performance liquid chromatography found reduction in cystine content was consistent with increased straightness and Product B (the no-lye relaxer) was found to be more effective and safer to use.
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References
Chromatography of Amino Acids on Sulfonated Polystyrene Resins. An Improved System
TL;DR: A systematic study has been made of the separations of amino acids by elution analysis on columns of synthetic ion exchange resins, demonstrating that synthetic resins are capable of separating most of the common amino acids from one another.
3.1K
On the determination of cystine as cysteic acid.
TL;DR: In this article, a reduction agent was used to destroy the excess performic acid before the initial reaction, and the subsequent exposure of cysteic acid residues to bromine would not be likely to be detrimental.
3K
Photometric ninhydrin method for use in the chromatography of amino acids.
Stanford Moore,William H. Stein +1 more
TL;DR: In the present investigations of the chromatographic separation of amino acids, it has been observed that, when the color development is carried out in tubes exposed to the air, these difficulties appear to result primarily from the influence of dissolved oxygen.
3K
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Sidney Udenfriend,Stanley Stein,Peter Böhlen,Wallace Dairman,Willy Leimgruber,Manfred Weigele +5 more
TL;DR: Fluorescamine is a new reagent for the detection of primary amines in the picomole range that is almost instantaneous at room temperature in aqueous media and the products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent.
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