Alterations in amounts and covalent modifications of low-molecular-weight chromosomal proteins in Chinese hamster ovary cells during polyamine depletion.

TL;DR: The results suggest that HMG I and HMG Y are HMG proteins specific for undifferentiated cells.

TL;DR: The results on the involvement of polyamines and their analogs in condensation of DNA and B to Z DNA transition correlate well with the conclusions drawn from experiments designed to observe the in vivo effects of polyamine and their Analogs on the growth of prokaryotic and eukaryotic cells.

TL;DR: A diploid yeast strain bearing two copies of disrupted spe1 lost heterozygosity at the mating-type locus more often when grown in the absence of polyamines than when growing in their presence, indicating that polyamine deficiency leads to either chromosome loss or to mitotic recombination.

TL;DR: The results indicate that modification of HMG 14 by phosphorylation at specific sites may have profound effects on its binding properties towards DNA and histone H1, and that HMG 17 has much weaker affinity for single-strandedDNA and hist one H1 than H MG 14.

TL;DR: The cell cycle of polyamine-limited cells was examined by using thymidine autoradiography, mitotic index analysis, and fraction labeled mitoses analysis and found that the longer doubling time of inhibitor-treated cultures was a consequence of increases in the lengths of the G1 and S phases.

TL;DR: It is suggested that under these conditions the direct synthesis of ribosomes from the particles may occur, and during this period preferential synthesis of Ribosomal protein took place.

TL;DR: Two-dimensional electrophoresis of protein fractions revealed that HMG-14 and H MG-17 are among the proteins that are retained by the single-stranded DNA column in 0.2 M NaCl/l mM Tris-Cl (pH 7.5), which suggests that those two proteins possess preferential affinity for single-Stranded DNA.

TL;DR: Deprivation of polyamines and ornithine causes in a polyamine-dependent CHO strain aneuploidy and alterations in nuclear morphology including micronuclei, macron nuclei, framented and bulged nuclei.