Journal Article10.1364/boe.510543
All-Optical dual module platform for motility-based functional scrutiny of microencapsulated probiotic bacteria
Zhe Wang,Giusy Giugliano,Jaromír Běhal,Michela Schiavo,Pasquale Memmolo,Lisa Miccio,Simonetta Grilli,filomena nazzaro,Pietro Ferraro,Vittorio Bianco +9 more
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TL;DR: A platform based on two label-free optical modules for rapidly screening and ranking probiotic candidates in the laboratory and 3D-tracks their motility profiles at a single element level to rank the strains is developed.
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Abstract: Probiotic bacteria are widely used in pharmaceutics to offer health benefits. Microencapsulation is used to deliver probiotics into the human body. Capsules in the stomach have to keep bacteria constrained until release occurs in the intestine. Once outside, bacteria must maintain enough motility to reach the intestine walls. Here, we develop a platform based on two label-free optical modules for rapidly screening and ranking probiotic candidates in the laboratory. Bio-speckle dynamics assay tests the microencapsulation effectiveness by simulating the gastrointestinal transit. Then, a digital holographic microscope 3D-tracks their motility profiles at a single element level to rank the strains.
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Citations
Motility-based scrutiny of probiotic bacteria in vitro digestive environment achieved by an all-optical platform
Zhe Wang,Giusy Giugliano,Jaromír Běhal,Michela Schiavo,Pasquale Memmolo,Lisa Miccio,Simonetta Grilli,Filomena Nazzaro,Pietro Ferraro,Vittorio Bianco +9 more
- 18 Jun 2025
TL;DR: Researchers developed an all-optical platform combining digital holography and bio-speckle decorrelation analysis to comprehensively analyze bacterial motility in vitro digestive environments, demonstrating its potential for evaluating probiotic bacteria strains.
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Colin Hill,Francisco Guarner,Gregor Reid,Glenn R. Gibson,Daniel Merenstein,Bruno Pot,Lorenzo Morelli,Roberto Berni Canani,Harry J. Flint,Seppo Salminen,Philip C. Calder,Mary Ellen Sanders +11 more
TL;DR: An expert panel was convened in October 2013 by the International Scientific Association for Probiotics and Prebiotics (ISAPP) to discuss the field of probiotics and the appropriate use and scope of the term probiotic.
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Taxonomy and important features of probiotic microorganisms in food and nutrition
TL;DR: Molecular typing methods such as pulsed-field gel electrophoresis, repetitive polymerase chain reaction, and restriction fragment length polymorphism are extremely valuable for specific characterization and detection of such strains selected for application as probiotics.
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