Aliphatic hydroxylation by highly purified liver microsomal cytochrome P-450. Evidence for a carbon radical intermediate
527
TL;DR: A very large isotope effect and a significant amount of epimerization for the hydroxylation of norbornane by cytochrome P-450, suggest an initial hydrogen abstraction to give a carbon radical intermediate.
read more
About: This article is published in Biochemical and Biophysical Research Communications. The article was published on 15 Mar 1978. and is currently open access. The article focuses on the topics: Hydroxylation & Hydrogen atom abstraction.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Alkane hydroxylation by peroxy acids: a comparison with the cytochrome P450 hydroxylation.
TL;DR: A comparable reaction sequence, initiated by homolytic peroxy bond cleavage, can be formulated for the alkane hydroxylation by the cytochrome P450 hydroperoxo-heme Compound 0.
8
Polycyclic hydrocarbon metabolism: a plethora of phenomena.
TL;DR: It is proposed that cytochrome P-450 may function to catalyze the oxidation of benzo(a)pyrene not only by a NADPH-dependent monooxygenation type reaction, but also concomitantly by a peroxidatic type reaction.
7
Alternative modes of O2 activation in P450 and NOS enzymes are clarified by DFT modeling and resonance Raman spectroscopy.
TL;DR: DFT modeling is used to examine the effects of H-bonding to either the terminal (Ot) or proximate (Op) atom of methylthiolate-Fe(II)porphine-O2, as well as to the thiolate S atom, supporting the interpretation of cytochrome P450 data by Kincaid and Sligar.
7
Understanding the Multifaceted Mechanism of Compound I Formation in Unspecific Peroxygenases through Multiscale Simulations.
Gustavo J Costa,Ruibin Liang +1 more
TL;DR: Multiscale simulations reveal the multifaceted mechanism of Compound I formation in unspecific peroxygenases, involving substrate-binding dehydration, proton transport, hydrogen bond rearrangement, and cosubstrate unbinding, which collectively enable efficient Compound I formation and inform biocatalyst design for sustainable chemical transformations.
7
References
•Book
Oxidases and Related Redox Systems
T.E. King,H.S. Mason,Chemical Pharmacology +2 more
- 01 Jul 1982
TL;DR: This book contains the proceedings of a symposium on oxidases and related redoxsystems, which covered topics such as Flavoprotein oxidase and oxygenases, Peroxidases, and Cytochrome P-450 and related proteins.
823
Purified liver microsomal cytochrome P-450. Separation and characterization of multiple forms.
TL;DR: It appears that the presence of these forms differing in subunit molecular weight may account for the variety of catalytic activities attributed to this pigment of liver microsomes.
464
Sodium periodate, sodium chlorite, organic hydroperoxides, and H2O2 as hydroxylating agents in steroid hydroxylation reactions catalyzed by partially purified cytochrome P-450
TL;DR: It was suggested that the ferryl ion (compound I) of cytochrome P-450 is the common “activated oxygen” species in these hydroxylations.
170