Journal Article10.3109/00365517209102747
Agarose gel electrophoresis.
1K
TL;DR: A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described, which could serve as a valuable analytical tool in protein chemistry.
read more
Abstract: A simple technique for agarose gel electrophoresis allowing the simultaneous separation of 15 samples in less than one hour is described. The method is very suitable for clinical routine analyses o...
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Inhibition of Elastase from Granulocytes by the Low Molecular Weight Bronchial Protease Inhibitor
K. Ohlsson,H. Tegner +1 more
TL;DR: It was demonstrated that the bronchial inhibitor formed firm complexes with granulocyte elastase but did not form complexes withgranulocyte collagenase.
The Neutral Proteases of Human Granulocytes
Kjell Ohlsson,Inge Odsson +1 more
TL;DR: Two electrophoretic variants of a collagenolytic enzyme from the cytoplasmic lysosome-like granules of leukaemic myeloid cells were purified by chromatography of the granule extract on Sephadex G-75, Bio Rex 70 and collagen–Sepharose, and electrophoresis on polyacrylamide gel.
Natural History and Life Expectancy in Severe Alpha1-Antitrypsin Deficiency, Pi Z
TL;DR: Smokers with severe alpha1-antitrypsin deficiency, Pi Z, had a significantly lower life expectancy than non-smokers and a greatly reduced survival, regardless of sex.
Purification of human C-reactive protein by barium sulfate and preparative agarose electrophoresis.
TL;DR: The described procedure has the advantage of not requiring either molecular sieve or affinity chromatography for purification of homogenous CRP from human sera.
"Non-secretory" plasma cell dyscrasia with normal serum immunoglobulins.
TL;DR: A well documented case of non-secretory myelomatosis with extensive skeletal lesions has been studied by immunohistologic techniques, electron microscopy, various electrophoretic procedures and different studies of serum and urine, concluding that the defect must be localized to the plasma cells themselves and not to the precursor cells of the B system.
References
•Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
318.1K
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
19.1K
Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies
TL;DR: The method utilizes the difference between the rate of electrophoretic migration of proteins and of their antibody complexes in agarose gel to perform quantitative analysis of proteins with a charge differing from that of the bulk of the immunoglobulins.
4.6K
Disc electrophoresis-i background and theory*
TL;DR: Some mechanisms that provide a rationale for the resolution afforded by zone electrophoresis in many gels will be detailed; the theory of some new modifications of zone electophoresis that have been designed to take maximum advantage of these mechanisms will be developed.
4.6K