Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex.
TL;DR: An improved technique for the preparation of ferritin-avidin conjugates for use as an ultrastructural marker in the localization of surface receptor sites on biotin-derivatized whole cells is described.
read more
About: This article is published in FEBS Letters. The article was published on 01 Oct 1976. and is currently open access. The article focuses on the topics: Biotinylation & Cytochemistry.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
Gary S. Wood,Roger A. Warnke +1 more
TL;DR: Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.
576
Bioactive egg compounds
Rainer Huopalahti,Rosina López-Fandino,Marc Anton,Rüdiger Schade +3 more
- 01 Jan 2007
TL;DR: Compounds with Antibacterial Activity, Egg-Protein-Derived Peptides with Antihypertensive Activity, and IgY Antibodies in Human and Veterinary Medicine are studied.
482
Introduction to avidin-biotin technology.
Meir Wilchek,Edward A. Bayer +1 more
TL;DR: The principles and advantages of the avidin–biotin system are discussed, which are based on the premise that if a compound biologically active with biotin is modified through its valeric acid side chain, the biological and physicochemical properties of the biotin-modified molecule are be changed significantly.
447
Patent
Method for protein-liposome coupling
Helen Loughrey,Pieter R. Cullis,Marcel B. Bally,Lewis S. L. Choi +3 more
- 17 Sep 1992
TL;DR: In this article, a method for synthesizing a substantially pure reactive lipid including N-[4-(p-maleimidophenyl)-butyryl]phosphatidylethanolamine (MPB-PE) and related compositions is described.
305
References
[20] Insolubilized biotin for the purification of avidin
Ed Bayer,Meir Wilchek +1 more
TL;DR: The purification of avidin by affinity chromatography is based upon the unusually strong complex formed by this protein with polymer-bound biotin, and the best method to date was accomplished by coupling biocytin to Sepharose.
81
Inactivation by avidin of biotin-modified bacteriophage
Jeffrey M. Becker,Meir Wilchek +1 more
TL;DR: The use of the technique of inactivation of chemically modified bacteriophage to the assay of a non-antibody protein and its binding ligand and free biotin inhibited the avidin in activation of T4-BIO is extended.
81
Sepharose-avidin column for the binding of biotin or biotin-containing peptides.
Agnes Bodanszky,Miklos Bodanszky +1 more
TL;DR: Avidin konnte an das Polysaccharid Sepharose 4B, das zuvor mit Bromcyan aktiviert wurde, gebunden werden, als besonders günstig für biotin oder Biotin-haltige Peptide.
Monovalent derivatives of concanavalin A.
TL;DR: Experiments indicated that the resultant preparations consisted mainly of monovalent dimers which showed subunit exchange to yield about 15% divalent dimers after 12 hr at physiological pH, which should allow a refined analysis of the effects of lectin valence at the cell surface.
46
Red cell agglutination kinetics: a method for automatic recording with the fragiligraph.
TL;DR: La différence de vitesse d'agglutination entre cellules jeunes and agées a été enregistrée.
35