Advances and Challenges in Small‐Molecule DNA Aptamer Isolation, Characterization, and Sensor Development
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TL;DR: Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind to specific molecules with high affinity, and offer a number of advantages relative to antibodies as biorecognition elements in biosensors as discussed by the authors.
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Abstract: Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind to specific molecules with high affinity, and offer a number of advantages relative to antibodies as biorecognition elements in biosensors However, it remains difficult and labor-intensive to develop aptamer-based sensors for small-molecule detection Here, we review the challenges and advances in the isolation and characterization of small-molecule-binding DNA aptamers and their use in sensors First, we discuss in vitro methodologies for the isolation of aptamers, and provide guidance on selecting the appropriate strategy for generating aptamers with optimal binding properties for a given application We next examine techniques for characterizing aptamer-target binding and structure Afterwards, we discuss various small-molecule sensing platforms based on original or engineered aptamers, and their detection applications Finally, we conclude with a general workflow to develop aptamer-based small-molecule sensors for real-world applications
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Citations
Biosensing with DNAzymes
TL;DR: A comprehensive review of biosensing with DNAzymes, providing an overview of different sensing applications while highlighting major progress and seminal contributions to the field of portable biosensor devices and point-of-care diagnostics is provided in this paper.
263
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment
TL;DR: Aptamers are short single-stranded DNA or RNA oligonucleotides isolated from randomized libraries, presenting high affinity and specificity toward targets with similar to antibodies as discussed by the authors .
102
Critical evaluation of aptamer binding for biosensor designs
Miriam Lucia Ojeda Arredondo
- 01 Jan 2022
TL;DR: A number of papers have been published questioning the binding ability of some popular aptamers such as those documented for As(III), ampicillin, chloramphenicol, isocarbophos, phorate and dopamine as mentioned in this paper .
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Biomolecular sensors for advanced physiological monitoring
Connor D. Flynn,Dingran Chang,Alam Md. mahmud,Hanie Yousefi,Jagotamoy Das,Kimberly T. Riordan,Edward H. Sargent,Shana O. Kelley +7 more
TL;DR: In this paper , the authors discuss approaches for the signal amplification of biomolecular sensors, including techniques to overcome Debye and mass transport limitations, and selectivity improvement, such as the integration of artificial affinity recognition elements.
80
Label-free and Dye-free Fluorescent Sensing of Tetracyclines Using a Capture-Selected DNA Aptamer.
TL;DR: In this article , the authors used DNA aptamers to detect tetracyclines and achieved a dissociation constant of 147 nM measured by isothermal titration calorimetry.
67
References
Innovative engineering and sensing strategies for aptamer-based small-molecule detection
TL;DR: This paper will review new approaches for the streamlined development of high-performance aptamer-based sensors for small-molecule detection, with emphasis placed on new aptamer engineering methods, the use of cooperative binding, and label-free approaches using fully-folded, high-affinity aptamers forSmall-Molecule sensing.
121
An RNA aptamer to the xanthine/guanine base with a distinctive mode of purine recognition
TL;DR: The ribonuclease sensitivities of some internal-loop residues changed upon the addition of xanthine, suggesting that the internal loop of the XBA RNA is involved in the ligand binding.
Graphene oxide-assisted non-immobilized SELEX of okdaic acid aptamer and the analytical application of aptasensor
TL;DR: The proposed ELAA method can be used to detect OA in seafood products with high sensitivity and can potentially be adapted for the determination of other small molecular analytes.
Generation and characterization of quinolone-specific DNA aptamers suitable for water monitoring
TL;DR: The selection of an aptamer pool with enhanced binding qualities for fluoroquinolones, a widely used group of antibiotics in both human and veterinary medicine, is described and it is suggested that these aptamers may be applicable as molecular receptors in biosensors or as catcher molecules in filter systems for improved monitoring and treatment of polluted water.
106
High-Affinity Nucleic-Acid-Based Receptors for Steroids
Kyung-Ae Yang,Hyo-Sun Chun,Yameng Zhang,Stevan Pecic,Nako Nakatsuka,Anne M. Andrews,Anne M. Andrews,Tilla S. Worgall,Milan N. Stojanovic +8 more
TL;DR: Three new classes of high affinity hydrophobic receptors for nonaromatic steroids based on deoxyribonucleotides are described, obtained through five high stringency selections coupled with tailored counter-selections, ensuring that these new aptamers will enable clinical chemistry applications.
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