Advances and Challenges in Small‐Molecule DNA Aptamer Isolation, Characterization, and Sensor Development
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TL;DR: Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind to specific molecules with high affinity, and offer a number of advantages relative to antibodies as biorecognition elements in biosensors as discussed by the authors.
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Abstract: Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind to specific molecules with high affinity, and offer a number of advantages relative to antibodies as biorecognition elements in biosensors However, it remains difficult and labor-intensive to develop aptamer-based sensors for small-molecule detection Here, we review the challenges and advances in the isolation and characterization of small-molecule-binding DNA aptamers and their use in sensors First, we discuss in vitro methodologies for the isolation of aptamers, and provide guidance on selecting the appropriate strategy for generating aptamers with optimal binding properties for a given application We next examine techniques for characterizing aptamer-target binding and structure Afterwards, we discuss various small-molecule sensing platforms based on original or engineered aptamers, and their detection applications Finally, we conclude with a general workflow to develop aptamer-based small-molecule sensors for real-world applications
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References
Mfold web server for nucleic acid folding and hybridization prediction
TL;DR: The objective of this web server is to provide easy access to RNA and DNA folding and hybridization software to the scientific community at large by making use of universally available web GUIs (Graphical User Interfaces).
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase
Craig Tuerk,Larry Gold +1 more
TL;DR: High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species.
10.4K
In vitro selection of RNA molecules that bind specific ligands.
TL;DR: Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules.
9.7K
•Proceedings Article
Fitting a mixture model by expectation maximization to discover motifs in biopolymers.
Timothy L. Bailey,Charles Elkan +1 more
- 01 Jan 1994
TL;DR: The algorithm described in this paper discovers one or more motifs in a collection of DNA or protein sequences by using the technique of expectation maximization to fit a two-component finite mixture model to the set of sequences.
The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update
Enis Afgan,Dannon Baker,Bérénice Batut,Marius van den Beek,Dave Bouvier,Martin Čech,John Chilton,Dave Clements,Nate Coraor,Björn Grüning,Aysam Guerler,Jennifer Hillman-Jackson,Saskia Hiltemann,Vahid Jalili,Helena Rasche,Nicola Soranzo,Jeremy Goecks,James Taylor,Anton Nekrutenko,Daniel Blankenberg +19 more
TL;DR: Improvements to Galaxy's core framework, user interface, tools, and training materials enable Galaxy to be used for analyzing tens of thousands of datasets, and >5500 tools are now available from the Galaxy ToolShed.
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