Adoptive Transfer of Bone Marrow-Derived Monocytes Ameliorates Schistosoma mansoni -Induced Liver Fibrosis in Mice
Veruska Cintia Alexandrino de Souza,Danielle Maria Nascimento Moura,Maria Carolina Accioly Brelaz de Castro,Patrícia T. Bozza,Ligia Almeida Paiva,Camila Juliet Barbosa Fernandes,Renata Lins Carneiro Leão,Jéssica Paula Lucena,Roni Evencio de Araujo,Alex José de Melo Silva,Regina Celia Bressan Queiroz de Figueiredo,Sheilla Andrade de Oliveira +11 more
TL;DR: The hypothesis that the predominance of M2reg macrophages, producers of immunosuppressive cytokines, may favor the improvement of hepatic fibrosis in a preclinical model is reinforced, through fibrous tissue remodeling, modulation of the inflammatory response and fibrogenesis.
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Abstract: Liver diseases are a major health problem worldwide leading to high mortality rates and causing a considerable economic burden in many countries. Cellular therapies as potential treatments for liver diseases have proven beneficial in most of the conditions. In recent years, studies involving therapy with bone marrow cells have been implemented to promote liver regeneration and to reduce hepatic fibrosis, however identifying the cell population present in the bone marrow that is responsible for hepatic improvement after therapy is still necessary. The aim of the present study was the evaluation of the therapeutic efficacy of monocytes obtained from bone marrow in fibrosis resulting from S. mansoni infection in C57BL/6 mice. Monocytes were isolated by immunomagnetic separation and administered to the infected animals. The effects of treatment were evaluated through morphometric, biochemical, immunological and molecular analyzes. Monocyte therapy promoted reduction of liver fibrosis induced by S. mansoni infection, associated with a decrease in production of inflammatory and pro-fibrogenic mediators. In addition, monocyte infusion caused downregulation of factors associated with the M1 activation profile, as well as upregulation of M2reg markers. The findings altogether reinforce the hypothesis that the predominance of M2reg macrophages, producers of immunosuppressive cytokines, may favor the improvement of hepatic fibrosis in a preclinical model, through fibrous tissue remodeling, modulation of the inflammatory response and fibrogenesis.
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INDUCTION AND REGULATION OF PATHOGENIC Th17 CELL RESPONSES IN SCHISTOSOMIASIS
Bridget Larkin,Patrick M. Smith,Holly Ponichtera,Mara G. Shainheit,Laura I. Rutitzky,Miguel J. Stadecker +5 more
TL;DR: New evidence intimately ties the development of severe pathology to IL-17-producing CD4 T helper (Th17) cells, a finding that adds a new dimension to the traditional CD4 Th1 vs. Th2 cell paradigm.
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Sheilla Andrade de Oliveira,Bruno Solano de Freitas Souza,Bruno Solano de Freitas Souza,Elton Sá Barreto,Carla Martins Kaneto,Hélio Almeida Neto,Hélio Almeida Neto,Carine Machado Azevedo,Carine Machado Azevedo,Elisalva Teixeira Guimarães,Luiz Antonio Rodrigues de Freitas,Luiz Antonio Rodrigues de Freitas,Ricardo Ribeiro-dos-Santos,Ricardo Ribeiro-dos-Santos,Milena Botelho Pereira Soares,Milena Botelho Pereira Soares +15 more
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