Additional file 4

TL;DR: In this article, the intensity of all the proteins in four cellulosomal fractions (CB I, CB II, MCC I and MCC II) were estimated by iBAQ and LFQ methods, in order to evaluate their quantity and abundance.

Abstract: The intensities of all the proteins in four cellulosomal fractions (CB I, CB II, MCC I and MCC II) were estimated by iBAQ and LFQ methods, in order to evaluate their quantity and abundance. The iBAQ intensities were normalized by dividing each value by that of the major primary scaffoldin ScaA1 in each sample, in order to facilitate interpretation of the results and relate the cellulosomal proteins to the major primary scaffoldin within the samples. The LFQ intensities were normalized by dividing the values by the intensity of ScaA1 in CB I, in order to simplify the fold-change analysis between the different samples. A. Identified scaffoldins in each fraction. B. 166 identified dockerincontaining proteins. CB, cellobiose; MCC, microcrystalline cellulose; Doc, dockerin; GH, glycoside hydrolase; CBM, carbohydrate-binding module; CE, carbohydrate esterase; PL, polysaccharide lyases. Significant iBAQ values (≥0.10) are shown in bold. The range of LFQ values is color coded according to the scale shown at the bottom.