Journal Article10.1021/NN800768Z
A simple, universal colorimetric assay for endonuclease/methyltransferase activity and inhibition based on an enzyme-responsive nanoparticle system.
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TL;DR: This new assay takes advantage of the palindromic recognition sequence of the restriction nucleases and the unique optical properties of AuNPs and is simpler than the procedure previously described by Xu et al.
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Abstract: An enzyme responsive nanoparticle system that uses a DNA−gold nanoparticle (AuNP) assembly as the substrate has been developed for the simple, sensitive, and universal monitoring of restriction endonucleases in real time. This new assay takes advantage of the palindromic recognition sequence of the restriction nucleases and the unique optical properties of AuNPs and is simpler than the procedure previously described by by Xu et al. (Angew. Chem. Int. Ed. Engl. 2007, 46, 3468−3470). Because it involves only one type of ssDNA modified AuNPs, this assay can be directed toward most of the endonucleases by simply changing the recognition sequence found within the linker DNA. In addition, the endonuclease activity could be quantitatively analyzed by the value of the reciprocal of hydrolysis half time (t1/2−1). Furthermore, our new design could also be applied to the assay of methyltransferase activity since the methylation of DNA inhibits its cleavage by the corresponding restriction endonuclease, and thus, thi...
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Citations
Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease.
TL;DR: A sensitive and selective approach for the DNA methyltransferase (MTase) activity assay and MTase inhibitor screening is reported by coupling the fluorescence quenching of graphene oxide with site-specific cleavage of a restriction endonuclease.
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Poly(ferrocenylsilane) electrolytes as a gold nanoparticle foundry: “two-in-one” redox synthesis and electrosteric stabilization, and sensing applications
Jing Song,Yen Nee Tan,Dominik Jańczewski,Mark A. Hempenius,Jianwei Xu,Hui Ru Tan,G. J. Vancso +6 more
TL;DR: Feasibility studies reported here indicate that the particles described can be applied as a colorimetric probe for the detection of redox molecules, e.g. vitamin C, in a controlled and facile manner.
A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis
Changfeng Zhu,Yanqin Wen,Hongzhen（彭红珍） Peng,Yi-Tao Long,Yao He,Qing（黄庆） Huang,Di（李迪） Li,Chunhai（樊春海） Fan +7 more
TL;DR: A strand displacement amplification DNA machine, which could be stimulated upon DNA methylation and then autonomously generates accumulated amounts of peroxidase-mimicking DNAzyme signaling machine products in an isothermal manner, is designed.
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Label-free molecular beacon-based quadratic isothermal exponential amplification: a simple and sensitive one-pot method to detect DNA methyltransferase activity.
Qingwang Xue,Lei Wang,Wei Jiang +2 more
TL;DR: A one-pot label-free molecular beacon-mediated quadratic isothermal exponential amplification strategy (LFMB-QIEA) for simple, rapid and sensitive DNA methyltransferase activity detection is developed.
27
Electrochemical biosensing method for the detection of DNA methylation and assay of the methyltransferase activity
TL;DR: The inhibition investigation demonstrated that fisetin could inhibit the T.aqI MTase activity with the IC50 value of 280 μM, therefore, the screening of the inhibitors of MTase could be accomplished using the novel method.
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