Journal Article10.1021/NN800768Z
A simple, universal colorimetric assay for endonuclease/methyltransferase activity and inhibition based on an enzyme-responsive nanoparticle system.
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TL;DR: This new assay takes advantage of the palindromic recognition sequence of the restriction nucleases and the unique optical properties of AuNPs and is simpler than the procedure previously described by Xu et al.
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Abstract: An enzyme responsive nanoparticle system that uses a DNA−gold nanoparticle (AuNP) assembly as the substrate has been developed for the simple, sensitive, and universal monitoring of restriction endonucleases in real time. This new assay takes advantage of the palindromic recognition sequence of the restriction nucleases and the unique optical properties of AuNPs and is simpler than the procedure previously described by by Xu et al. (Angew. Chem. Int. Ed. Engl. 2007, 46, 3468−3470). Because it involves only one type of ssDNA modified AuNPs, this assay can be directed toward most of the endonucleases by simply changing the recognition sequence found within the linker DNA. In addition, the endonuclease activity could be quantitatively analyzed by the value of the reciprocal of hydrolysis half time (t1/2−1). Furthermore, our new design could also be applied to the assay of methyltransferase activity since the methylation of DNA inhibits its cleavage by the corresponding restriction endonuclease, and thus, thi...
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Citations
Highly sensitive fluorescence assay of DNA methyltransferase activity by methylation-sensitive cleavage-based primer generation exponential isothermal amplification-induced G-quadruplex formation.
Qingwang Xue,Yanqin Lv,Shuling Xu,Yuanfu Zhang,Lei Wang,Rui Li,Qiaoli Yue,Haibo Li,Xiaohong Gu,Shuqiu Zhang,Jifeng Liu,Jifeng Liu +11 more
TL;DR: A highly sensitive fluorescence assay of DNA methyltransferase by methylation-sensitive cleavage-based primer generation exponential isothermal amplification (PG-EXPA) coupled with supramolecular fluorescent Zinc(II)-protoporphyrin IX (ZnPPIX)/G-quadruplex and the application of the proposed sensing strategy for screening Dam MTase inhibitors was demonstrated with satisfactory results.
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A Host-Guest Interaction-Based and Metal-Organic Gel-Based Biosensor with Aggregation-Induced Electrochemiluminescence Enhancement for Methyltransferase Assay.
TL;DR: For the first time, a host-guest interaction-based and MOG-based biosensor with aggregation-induced electrochemiluminescence (ECL) enhancement for M.SssI methyltransferase (M.SSSI MTase) assay is developed.
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A bioluminescence assay for DNA methyltransferase activity based on methylation-resistant cleavage
TL;DR: A new bioluminescence strategy based on methylation-resistant cleavage and protein expression in vitro to detect DNA MTase activity and could be extended to the detection of other DNA MTases and the corresponding inhibitor screening.
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Nanomaterial-based biosensors for DNA methyltransferase assay.
TL;DR: Recent progress in the development of nanomaterial-based biosensors for DNA methyltransferase assay is summarized including the strategies, features and applications, and the future direction and challenges in this area are highlighted.
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A colorimetric assay of DNA methyltransferase activity based on peroxidase mimicking of DNA template Ag/Pt bimetallic nanoclusters.
Hanie Ahmadzade Kermani,Morteza Hosseini,Andrea Miti,Mehdi Dadmehr,Giampaolo Zuccheri,Saman Hosseinkhani,Mohammad Reza Ganjali +6 more
TL;DR: A novel colorimetric method to assay M.SssI MTase activity employing peroxidase-like activity of DNA template Ag/Pt NCs without using restriction enzymes is developed and can be used for the evaluation and screening of inhibitors for DNA MTase.
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References
Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles
TL;DR: A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported, which can detect about 10 femtomoles of an oligonucleotide.
One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes
TL;DR: In this new nanoparticle-based detection system, Au particles are used to complex a 24-base polynucleotide target and exhibit characteristic, exceptionally sharp “melting transitions” which allows one to distinguish target sequences that contain one base end mismatches, deletions, or an insertion from the fully complementary target.
2.3K
What controls the melting properties of DNA-linked gold nanoparticle assemblies?
TL;DR: A series of experiments and a theoretical model designed to systematically define and evaluate the relative importance of nanoparticle, oligonucleotide, and environmental variables that contribute to the observed sharp melting transitions associated with DNA-linked nanoparticle structures are reported.
1.5K
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Juewen Liu,Yi Lu +1 more
TL;DR: The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.
1.3K
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