A simple headspace equilibration method for measuring dissolved methane
Cédric Magen,Cédric Magen,Laura L. Lapham,John W. Pohlman,Kathleen S. Marshall,Samantha Bosman,Michael A. Casso,Jeffrey P. Chanton +7 more
TL;DR: In this article, the authors revisited the headspace equilibration technique and described a simple, inexpensive, and reliable method to measure methane in fresh and seawater, regardless of concentration.
read more
Abstract: Dissolved methane concentrations in the ocean are close to equilibrium with the atmosphere. Because methane is only sparingly soluble in seawater, measuring it without contamination is challenging for samples collected and processed in the presence of air. Several methods for analyzing dissolved methane are described in the literature, yet none has conducted a thorough assessment of the method yield, contamination issues during collection, transport and storage, and the effect of temperature changes and preservative. Previous extraction methods transfer methane from water to gas by either a "sparge and trap" or a "headspace equilibration" technique. The gas is then analyzed for methane by gas chromatography. Here, we revisit the headspace equilibration technique and describe a simple, inexpensive, and reliable method to measure methane in fresh and seawater, regardless of concentration. Within the range of concentrations typically found in surface seawaters (2-1000 nmol L-1), the yield of the method nears 100% of what is expected from solubility calculation following the addition of known amount of methane. In addition to being sensitive (detection limit of 0.1 ppmv, or 0.74 nmol L-1), this method requires less than 10 min per sample, and does not use highly toxic chemicals. It can be conducted with minimum materials and does not require the use of a gas chromatograph at the collection site. It can therefore be used in various remote working environments and conditions.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Widespread methane seepage along the continental margin off Svalbard - from Bjørnøya to Kongsfjorden
Susan Mau,Miriam Römer,Marta E Torres,Ingeborg Bussmann,Thomas Pape,Ellen Damm,Patrizia Geprägs,Paul Wintersteller,Chieh-Wei Hsu,Markus Loher,Gerhard Bohrmann +10 more
TL;DR: Findings of a much broader seepage area extending from 74° to 79° are reported, where more than a thousand gas discharge sites were imaged as acoustic flares, and it is postulate that the gas ascends along this fracture zone.
Contribution of oxic methane production to surface methane emission in lakes and its global importance.
Marco Günthel,Daphne Donis,Georgiy Kirillin,Danny Ionescu,Mina Bizic,Daniel Frank Mcginnis,Hans-Peter Grossart,Hans-Peter Grossart,Kam W. Tang +8 more
TL;DR: Unpredictable quantities of this greenhouse gas are produced in lake surfaces, indicating an overlooked global importance from oxygenated sources, according to Günthel and colleagues.
Methane- and dissolved organic carbon-fueled microbial loop supports a tropical subterranean estuary ecosystem.
David Brankovits,John W. Pohlman,John W. Pohlman,Helge Niemann,Mary Beth Leigh,Mary-Cathrine Leewis,Mary-Cathrine Leewis,Kevin W. Becker,Thomas M. Iliffe,Fernando Alvarez,Moritz F. Lehmann,Bil Phillips +11 more
TL;DR: It is demonstrated that a microbial loop fuelled by methane and dissolved organic carbon sustains the anchialine food web in the Yucatan Peninsula (Mexico), revealing a heretofore unrecognized subterranean methane sink and contributing to the understanding of the carbon cycle and ecosystem function of karst subterranean estuaries.
Organic matter cycling across the sulfate-methane transition zone of the Santa Barbara Basin, California Borderland
Tomoko Komada,David J. Burdige,Huan-Lei Li,Cédric Magen,Cédric Magen,Jeffrey P. Chanton,Abraham K. Cada +6 more
TL;DR: In this article, the authors investigated organic matter cycling in the sulfate-methane transition zone (SMTZ) of the organic-rich sediments of the Santa Barbara Basin, California Borderland, and examined the occurrence of organoclastic SO42− reduction (oSR) within this zone using bulk solute profiles and Δ14C and δ13C values of selected carbon pools.
93
Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea
Shahjahon Begmatov,Alexander S Savvichev,Vitaly V. Kadnikov,Alexey V. Beletsky,Igor I Rusanov,A. A. Klyuvitkin,E. A. Novichkova,Andrey V. Mardanov,Nikolai V. Pimenov,Nikolai V. Ravin +9 more
- 15 Nov 2021
TL;DR: A combination of physicochemical and radiotracer analysis, high-throughput sequencing of the 16S rRNA, and particulate methane monooxygenase subunit A (pmoA) genes was used to link a microbial community profile with methane, sulfur, and nitrogen cycling processes.
58
References
American Society for Testing and Materials
S.R. Esterby
- 31 Oct 2001
TL;DR: The American Society for Testing and Materials (ASTM) as mentioned in this paper is an independent organization devoted to the development of standards for testing and materials, and is a member of IEEE 802.11.
3.9K
Oceanic methane biogeochemistry.
TL;DR: It is shown that thermodynamic and kinetic constraints largely prevent large-scale methanogenesis in the open ocean water column, and the role of anaerobic oxidation of methane has changed from a controversial curiosity to a major sink in anoxic basins and sediments.
Solubility of methane in distilled water and seawater
TL;DR: In this paper, a table of Bunsen solubility coefficients covering the temperature range -2' to 3OoC and the salinity range -0-40 parts per thousand was calculated from the fitted equation.
757
Methane consumption in cariaco trench waters and sediments
TL;DR: In this article, the water column and the sediments of the Cariaco Trench were measured and the results showed that CH4 is non-conservative in both environments, and the concentrations of CH4 were found to be non-consistency.
593
Anaerobic methane oxidation rates at the sulfate‐methane transition in marine sediments from Kattegat and Skagerrak (Denmark)
TL;DR: In this paper, the in situ rates of sulfate reduction and anaerobic methane oxidation in 2-3m-long sediment cores were made, with a broad maximum below the sediment surface and a smaller, narrow maximum at the sulfate-methane transition.
575