A Shift from Reversible to Irreversible X Inactivation Is Triggered during ES Cell Differentiation
Anton Wutz,Rudolf Jaenisch +1 more
TL;DR: A full-length mouse Xist cDNA transgene and an inducible expression system facilitating controlled Xist expression in ES cells and differentiated cultures are generated, suggesting that reversible repression by Xist is a required initiation step that might occur during normal X inactivation in female cells.
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About: This article is published in Molecular Cell. The article was published on 01 Apr 2000. and is currently open access. The article focuses on the topics: XIST & Tsix.
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Citations
Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci
Daniel Smeets,Daniel Smeets,Yolanda Markaki,Volker Schmid,Felix Kraus,Felix Kraus,Anna Tattermusch,Andrea Cerase,Michael Sterr,Susanne Fiedler,Justin Demmerle,Jens Popken,Heinrich Leonhardt,Neil Brockdorff,Thomas Cremer,Lothar Schermelleh,Lothar Schermelleh,Marion Cremer +17 more
TL;DR: 3D-SIM provides experimental evidence for profound differences between the functional architecture of transcriptionally active CTs and the Barr body, and proposes that the PR and macromolecular complexes in IC channels together form the transcriptionally permissive active nuclear compartment (ANC).
X-Chromosome Inactivation: A Crossroads Between Chromosome Architecture and Gene Regulation
TL;DR: Current knowledge of the 3D organization of the mammalian X-chromosome chromatin is reviewed and its relationship with gene activity in light of the initiation, spreading, and maintenance of XCI, as well as escape from gene silencing is discussed.
Drosophila dosage compensation: a complex voyage to the X chromosome.
TL;DR: This review will discuss the relative contributions of sequence elements and transcriptional marks to the complete pattern of MSL complex binding and suggest that this occurs through a multi-step targeting mechanism that involves DNA sequences elements and epigenetic marks associated with transcription.
The X-inactivation trans-activator Rnf12 is negatively regulated by pluripotency factors in embryonic stem cells
TL;DR: It is reported that Rnf12, a third X-linked gene critical for the regulation of X-inactivation, is under the control of Nanog, Oct4 and Sox2, the three factors lying at the heart of the pluripotency network.
Long nonoding RNAs in the X-inactivation center.
TL;DR: The X-inactivation center is a hotbed of functional long noncoding RNAs thought to help orchestrate the epigenetic transcriptional states of the two X-chromosomes in females as well as of the single X- chromosome in males.
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Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
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Transcriptional activation by tetracyclines in mammalian cells
Manfred Gossen,Sabine Freundlieb,Gabriele Bender,Gerhard Müller,Wolfgang Hillen,Hermann Bujard +5 more
TL;DR: Adding doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold.
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A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome
Carolyn J. Brown,Andrea Ballabio,James L. Rupert,Ronald G. Lafreniere,Markus Grompe,Rossana Tonlorenzi,Huntington F. Willard +6 more
TL;DR: This gene, called XIST (for Xi-specific transcripts), is a candidate for a gene either involved in or uniquely influenced by the process of X inactivation, and is described as an X-linked gene with a novel expression pattern.
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