A Shift from Reversible to Irreversible X Inactivation Is Triggered during ES Cell Differentiation
Anton Wutz,Rudolf Jaenisch +1 more
TL;DR: A full-length mouse Xist cDNA transgene and an inducible expression system facilitating controlled Xist expression in ES cells and differentiated cultures are generated, suggesting that reversible repression by Xist is a required initiation step that might occur during normal X inactivation in female cells.
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About: This article is published in Molecular Cell. The article was published on 01 Apr 2000. and is currently open access. The article focuses on the topics: XIST & Tsix.
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Citations
Marsupial Genetics Reveals Insights into Evolution of Mammalian X Chromosome Inactivation
Shafagh Al Nadaf,Paul D. Waters,Janine E. Deakin,Jennifer A. Marshall Graves +3 more
- 01 Jan 2010
TL;DR: The molecular similarities and differences between X-inactivation in marsupials and eutherians are highlighted, and the partial dosage compensation system observed in monotreme mammals, which appears more similar to bird dosage compensation is compared.
1
A RIF1/KAP1-based toggle switch stabilises the identities of the inactive and active X chromosomes during X inactivation
Elin Enervald,Elin Enervald,Lynn M. Powell,Lora Boteva,Rossana Foti,Nerea Blanes Ruiz,Gözde Kibar,Agnieszka Piszczek,Fatima Cavaleri,Martin Vingron,Andrea Cerase,Sara B.C. Buonomo,Sara B.C. Buonomo +12 more
TL;DR: In this article, the authors show that upregulation of the long non-coding RNA Tsix weakens the symmetric RIF1 association with the Xist promoter, and opens a window of opportunity for a more stable association of KAP1.
Role of XIST RNA and its interacting protein partners in gene silencing
Jakub Minks
- 01 Jan 2012
TL;DR: The present work aims to provide a chronology of the main events leading up to and during the development of EMT and aims to clarify the sequence of events leading to and after EMT.
Induced pluripotent stem cells versus embryonic stem cells: a comprehensive overview of differences and similarities
Stefania Scalise,Luana Scaramuzzino,Valeria Lucchino,Elvira Immacolata Parrotta,G. Cuda +4 more
- 01 Jan 2021
TL;DR: Comparison studies show that although iPSCs are very similar to ESCs, they are still characterized by some specific molecular signatures that need to be further investigated to improve the use of iPSC in basic research and clinical settings.
1
Maternal SMCHD1 controls both imprinted Xist expression and imprinted X chromosome inactivation
Iromi Wanigasuriya,Iromi Wanigasuriya,Sarah Kinkel,Sarah Kinkel,Tamara Beck,Tamara Beck,Ellise A Roper,Kelsey Breslin,Kelsey Breslin,Heather J. Lee,Andrew Keniry,Andrew Keniry,Matthew E. Ritchie,Matthew E. Ritchie,Marnie E. Blewitt,Marnie E. Blewitt,Quentin Gouil,Quentin Gouil +17 more
TL;DR: This article showed that maternal epigenetic repressor SMCHD1 is required not only for the imprinted expression of Xist in pre-plantation embryos, but also for the efficient silencing of the inactive X in both the preimplantation embryo and mid-gestation placenta.
References
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Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
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Transcriptional activation by tetracyclines in mammalian cells
Manfred Gossen,Sabine Freundlieb,Gabriele Bender,Gerhard Müller,Wolfgang Hillen,Hermann Bujard +5 more
TL;DR: Adding doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold.
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A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome
Carolyn J. Brown,Andrea Ballabio,James L. Rupert,Ronald G. Lafreniere,Markus Grompe,Rossana Tonlorenzi,Huntington F. Willard +6 more
TL;DR: This gene, called XIST (for Xi-specific transcripts), is a candidate for a gene either involved in or uniquely influenced by the process of X inactivation, and is described as an X-linked gene with a novel expression pattern.
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