A Shift from Reversible to Irreversible X Inactivation Is Triggered during ES Cell Differentiation
Anton Wutz,Rudolf Jaenisch +1 more
TL;DR: A full-length mouse Xist cDNA transgene and an inducible expression system facilitating controlled Xist expression in ES cells and differentiated cultures are generated, suggesting that reversible repression by Xist is a required initiation step that might occur during normal X inactivation in female cells.
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About: This article is published in Molecular Cell. The article was published on 01 Apr 2000. and is currently open access. The article focuses on the topics: XIST & Tsix.
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Citations
How Many Non-coding RNAs Does It Take to Compensate Male/Female Genetic Imbalance?
TL;DR: This chapter describes the processes coping with sex chromosome genetic imbalance and how ncRNAs underlie dosage compensation mechanisms and influence male-female differences in mammals and discusses how n cRNAs have been tinkered with during therian evolution to adapt XCI mechanistic to species-specific constraints.
4
Nuclear Reprogramming: Biological and Technological Constraints
Kevin Eggan,Rudolf Jaenisch +1 more
- 01 Jan 2002
TL;DR: This chapter focuses on the nuclearReprogramming process and how both the technological parameters of the cloning process and the biological limitation of nuclear reprogramming might relate to the inefficiencies and phenotypes observed during the development of cloned animals.
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Xist spatially amplifies SHARP recruitment to balance chromosome-wide silencing and specificity to the X chromosome
Joanna W. Jachowicz,Mackenzie Strehle,Abhik Banerjee,Abhik Banerjee,Jasmine Thai,Mario Blanco,Mitchell Guttman +6 more
TL;DR: In this paper, the authors demonstrate a spatial amplification mechanism that allows Xist to achieve two essential but countervailing regulatory objectives: chromosome-wide gene silencing and specificity to the X.
4
•Dissertation
Identification of tissue specific differential methylation in human body fluids and its potential application in forensics.
Farzeen. Kader
- 01 Jan 2015
TL;DR: In this article, the authors proposed a method to solve the problem of "missing links".2.62 2.62 3.0 2.2 2.3 2.5
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•Dissertation
Keeping the genome in shape : a role for protein and RNA
Erik Splinter
- 25 Oct 2011
TL;DR: The protein CTCF and the noncoding Xist RNA were identified as factors involved in chromatin folding, each acting at a different level of organization, and providing novel insights into the mechanisms by which DNA is organized inside the nucleus of a cell.
4
References
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Manipulating the mouse embryo: A laboratory manual
Brigid L.M. Hogan,Frank Costantini,Elizabeth Lacy +2 more
- 01 Jan 1986
TL;DR: Here are recorded the tech- niques for preparing, inserting and analysing DNA sequences, for retroviral infection of mice, for production and use of EC and EK cells as vehicles for engineered sequences and for nuclear transplantation - all against a background of the basic procedures required for pro- ducing and handling the em- bryos.
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Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
3.7K
Transcriptional activation by tetracyclines in mammalian cells
Manfred Gossen,Sabine Freundlieb,Gabriele Bender,Gerhard Müller,Wolfgang Hillen,Hermann Bujard +5 more
TL;DR: Adding doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold.
2.8K
A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome
Carolyn J. Brown,Andrea Ballabio,James L. Rupert,Ronald G. Lafreniere,Markus Grompe,Rossana Tonlorenzi,Huntington F. Willard +6 more
TL;DR: This gene, called XIST (for Xi-specific transcripts), is a candidate for a gene either involved in or uniquely influenced by the process of X inactivation, and is described as an X-linked gene with a novel expression pattern.
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