A Shift from Reversible to Irreversible X Inactivation Is Triggered during ES Cell Differentiation
Anton Wutz,Rudolf Jaenisch +1 more
TL;DR: A full-length mouse Xist cDNA transgene and an inducible expression system facilitating controlled Xist expression in ES cells and differentiated cultures are generated, suggesting that reversible repression by Xist is a required initiation step that might occur during normal X inactivation in female cells.
read more
About: This article is published in Molecular Cell. The article was published on 01 Apr 2000. and is currently open access. The article focuses on the topics: XIST & Tsix.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency.
Maxim Van Cleef Greenberg,Aurélie Teissandier,Marius Walter,Daan Noordermeer,Déborah Bourc'his +4 more
TL;DR: It is shown that 3D regulation occurs upstream of the polycomb and DNA methylation pathways, and the importance of multi-layered regulatory frameworks to ensure proper spatio-temporal activation of developmentally important genes is revealed.
15
Maintenance of epigenetic information: a noncoding RNA perspective
TL;DR: This review discusses the prospective role of ncRNAs in the area of inheritance and maintenance of these established silenced states and attempts to understand and speculate how these RNA dependent or independent maintenance mechanisms differ between each other in a developmental stage, tissue, and gene-specific manner in different biological contexts.
15
The Talented LncRNAs: Meshing into Transcriptional Regulatory Networks in Cancer
Dana Segal,Josée Dostie +1 more
TL;DR: Long non-coding RNAs (lncRNAs) are a large class of RNA molecules known to regulate the expression of genes as mentioned in this paper , which can act as oncogenes or tumor suppressors.
X-chromosome inactivation: lessons from transgenic mice.
TL;DR: This review describes recent experiments aimed at understanding the first events of XCI and proposes a model for initiation of X CI, which involves counting the X chromosomes in a cell, randomly choosing those to be inactivated, spreading the inactivation signal in cis throughout the chromosome.
14
The Lac repressor provides a reversible gene expression system in undifferentiated and differentiated embryonic stem cell
TL;DR: This reversible system is the first to function from undifferentiated to individual welldifferentiated ES cells, providing a very useful tool to understand molecular mechanisms underlying ES cell self-renewal, commitment and differentiation.
14
References
•Book
Manipulating the mouse embryo: A laboratory manual
Brigid L.M. Hogan,Frank Costantini,Elizabeth Lacy +2 more
- 01 Jan 1986
TL;DR: Here are recorded the tech- niques for preparing, inserting and analysing DNA sequences, for retroviral infection of mice, for production and use of EC and EK cells as vehicles for engineered sequences and for nuclear transplantation - all against a background of the basic procedures required for pro- ducing and handling the em- bryos.
5.7K
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
3.7K
Transcriptional activation by tetracyclines in mammalian cells
Manfred Gossen,Sabine Freundlieb,Gabriele Bender,Gerhard Müller,Wolfgang Hillen,Hermann Bujard +5 more
TL;DR: Adding doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold.
2.8K
A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome
Carolyn J. Brown,Andrea Ballabio,James L. Rupert,Ronald G. Lafreniere,Markus Grompe,Rossana Tonlorenzi,Huntington F. Willard +6 more
TL;DR: This gene, called XIST (for Xi-specific transcripts), is a candidate for a gene either involved in or uniquely influenced by the process of X inactivation, and is described as an X-linked gene with a novel expression pattern.
1.5K