Journal Article10.1007/S10295-018-02121-X
A selective genome-guided method for environmental Burkholderia isolation.
F. P. Jake Haeckl,João L. Baldim,Dasha Iskakova,Kenji L. Kurita,Marisi G. Soares,Roger G. Linington +5 more
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TL;DR: This method uses a combination of selective agar isolation media and multiplexed PCR profiling to derive Burkholderia strains from environmental samples with 95% efficiency and offers new insight into potential methods for selective isolation method development for other priority genera.
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Abstract: The genus Burkholderia is an emerging source of novel natural products chemistry, yet to date few methods exist for the selective isolation of strains of this genus from the environment. More broadly, tools to efficiently design selection media for any given genus would be of significant value to the natural products and microbiology communities. Using a modification of the recently published SMART protocol, we have developed a two-stage isolation protocol for strains from the genus Burkholderia. This method uses a combination of selective agar isolation media and multiplexed PCR profiling to derive Burkholderia strains from environmental samples with 95% efficiency. Creation of this new method paves the way for the systematic exploration of natural products chemistry from this important genus and offers new insight into potential methods for selective isolation method development for other priority genera.
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Citations
Natural products in drug discovery: advances and opportunities.
TL;DR: In this article, the authors summarize recent technological developments that are enabling natural product-based drug discovery, highlight selected applications and discuss key opportunities, and discuss the potential of using natural products as drug leads.
•Dissertation
Detection of the Burkholderia cepacia complex in soil environments
Suzanne C. McKenzie Miller
- 01 Jun 2001
TL;DR: Based on PCR detection, Bcc appears to be prevalent in soil from urban and suburban environments, and culture-based recovery of Bcc may underestimate environmental populations.
Collateral sensitivity profiling in drug-resistant Escherichia coli identifies natural products suppressing cephalosporin resistance
Dennis Y. Liu,Laura Phillips,Darryl Wilson,Kelly M. Fulton,Susan M. Twine,Alex Wong,Roger G. Linington +6 more
TL;DR: In this paper , the authors present a high-throughput screening platform termed Collateral Sensitivity Profiling (CSP) to map the difference in bioactivity of large chemical libraries across 29 drug-resistant strains of E. coli.
Custom Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometric Database for Identification of Environmental Isolates of the Genus Burkholderia and Related Genera
Claire H. Fergusson,Julienne M. F. Coloma,Mercia C. Valentine,F. P. Jake Haeckl,Roger G. Linington +4 more
TL;DR: A fast, accurate method for taxonomic assignment of environmentally derived bacteria from the order Burkholderiales is presented, providing a valuable alternative to traditional PCR-based methods.
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Methodological tools to study species of the genus Burkholderia.
Viola Camilla Scoffone,Gabriele Trespidi,Giulia Barbieri,Samuele Irudal,Aygun Israyilova,Silvia Buroni +5 more
TL;DR: In this paper, the authors reviewed the techniques used in the last few years to update the taxonomy of the genus, to study gene functions and regulations, to deepen the knowledge on the drug resistance which characterizes these bacteria, and to elucidate their mechanisms to establish infections.
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