A RhoA-FRET biosensor mouse for intravital imaging in normal tissue homeostasis and disease contexts
Max Nobis,David Herrmann,Sean C. Warren,Shereen Kadir,Wilfred Leung,Monica J. Killen,Astrid Magenau,David A. Stevenson,Morghan C. Lucas,Nadine Reischmann,Claire Vennin,James R.W. Conway,Alice Boulghourjian,Anaiis Zaratzian,Andrew M. K. Law,David Gallego-Ortega,Christopher J. Ormandy,Stacey N. Walters,Shane T. Grey,Jacqueline Bailey,Tatyana Chtanova,Julian M. W. Quinn,Paul A. Baldock,Peter I. Croucher,Juliane P. Schwarz,Agata Mrowinska,Lei Zhang,Herbert Herzog,Andrius Masedunskas,Edna C. Hardeman,Peter W. Gunning,Gonzalo del Monte-Nieto,Gonzalo del Monte-Nieto,Richard P. Harvey,Richard P. Harvey,Michael S. Samuel,Marina Pajic,Ewan J. McGhee,Anna-Karin E. Johnsson,Owen J. Sansom,Heidi C.E. Welch,Jennifer P. Morton,Douglas Strathdee,Kurt I. Anderson,Paul Timpson +44 more
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TL;DR: The generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo in real time are described.
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About: This article is published in Cell Reports. The article was published on 03 Oct 2017. and is currently open access. The article focuses on the topics: RHOA.
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TL;DR: It is found that frequent and diverse somatic aberrations in genes described traditionally as embryonic regulators of axon guidance, particularly SLIT/ROBO signalling, are also evident in murine Sleeping Beauty transposon-mediated somatic mutagenesis models of pancreatic cancer, providing further supportive evidence for the potential involvement ofAxon guidance genes in pancreatic carcinogenesis.
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Cancer Invasion and the Microenvironment: Plasticity and Reciprocity
TL;DR: The cell-matrix and cell-cell adhesion, protease, and cytokine systems that underlie tissue invasion by cancer cells are described and explained to explain how the reciprocal reprogramming of both the tumor cells and the surrounding tissue structures not only guides invasion, but also generates diverse modes of dissemination.
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Induction of mammary tumors by expression of polyomavirus middle T oncogene: a transgenic mouse model for metastatic disease.
TL;DR: Results suggest that middle T antigen acts as a potent oncogene in the mammary epithelium and that cells that express it possess an enhanced metastatic potential.
Rho GTPases Control Polarity, Protrusion, and Adhesion during Cell Movement
Catherine D. Nobes,Alan Hall +1 more
TL;DR: It is concluded that the signal transduction pathways controlled by the four small GTPases, Rho, Rac, Cdc42, and Ras, cooperate to promote cell movement.
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RHO-GTPases and cancer.
TL;DR: The RAS oncogenes are members of a large family of small GTPases that bind GTP and hydrolyse it to GDP and the switching between these two states regulates a wide range of cellular processes.
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