A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris
TL;DR: It is proposed that the wood-rotting basidiomycete acquires biochemical energy by oxidizing glucose to oxalate by the occurrence of the unprecedented metabolic coupling of the TCA and glyoxylate cycles that support oxalic acid biosynthesis.
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Abstract: A metabolic mechanism for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris has been proposed on the basis of biochemical analyses of glucose metabolism. There was a strong correlation between glucose consumption and oxalate production. Oxalic acid was found to accumulate in the culture fluid in about 80% of the theoretical yield or about 5-fold, on the basis of the fungal biomass harvested. The results clearly indicate that glucose was not completely oxidized to CO(2) by the tricarboxylic acid (TCA) cycle but converted mainly to oxalate. The determination of the 12 enzymes concerned has revealed the occurrence of the unprecedented metabolic coupling of the TCA and glyoxylate cycles that support oxalate biosynthesis. In this metabolic system, isocitrate lyase (EC ), together with oxaloacetase (EC ), was found to play a pivotal role in yielding oxalate from oxaloacetate via the acetate-recycling routes. Moreover, malate dehydrogenase (EC ), with an extraordinarily high activity among the enzymes tested, was shown to play an important role in generating NADH by oxidation of malate to oxaloacetate. Thus, it is proposed that the wood-rotting basidiomycete acquires biochemical energy by oxidizing glucose to oxalate.
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Citations
Oxalate production by fungi: significance in geomycology, biodeterioration and bioremediation
Geoffrey M. Gadd,Geoffrey M. Gadd,Jaleh Bahri-Esfahani,Jaleh Bahri-Esfahani,Qianwei Li,Young Joon Rhee,Zhan Wei,Marina Fomina,Marina Fomina,Xinjin Liang +9 more
TL;DR: The importance of oxalate in geomycology, biodeterioration, environmental biotechnology and bioremediation is discussed in this article, where salient information from environmental and applied research is drawn together to emphasize the importance of Oxalate.
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A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris
TL;DR: It is proposed that the wood-rotting basidiomycete acquires biochemical energy by oxidizing glucose to oxalate by the occurrence of the unprecedented metabolic coupling of the TCA and glyoxylate cycles that support oxalic acid biosynthesis.
181
Mechanisms of Broad Host Range Necrotrophic Pathogenesis in Sclerotinia sclerotiorum.
Xiaofei Liang,Jeffrey A. Rollins +1 more
TL;DR: A two-phase infection model is proposed in which the pathogen first evades, counteracts and subverts host basal defense reactions prior to killing and degrading host cells, to provide the basis for understanding corresponding host resistance.
162
Oxaloacetate acetylhydrolase gene mutants of Sclerotinia sclerotiorum do not accumulate oxalic acid, but do produce limited lesions on host plants
Xiaofei Liang,Daniele Liberti,Moyi Li,Young Tae Kim,Andrew Hutchens,Ronald Wilson,Jeffrey A. Rollins +6 more
TL;DR: The experimental data establish a critical requirement for OAH activity in S. sclerotiorum biogenesis and pathogenesis, but also suggest that factors independent of OA contribute to the establishment of primary lesions.
131
Analysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood
Chiaki Hori,Takuya Ishida,Kiyohiko Igarashi,Masahiro Samejima,Hitoshi Suzuki,Emma R. Master,Patricia Ferreira,Francisco J. Ruiz-Dueñas,Benjamin W. Held,Paulo Canessa,Luis F. Larrondo,Monika Schmoll,Irina S. Druzhinina,Christian P. Kubicek,Jill Gaskell,Phil Kersten,Franz J. St John,Jeremy D. Glasner,Grzegorz Sabat,Sandra Splinter BonDurant,Khajamohiddin Syed,Jagjit S. Yadav,Anthony C. Mgbeahuruike,Andriy Kovalchuk,Fred O. Asiegbu,Gerald Lackner,Dirk Hoffmeister,Jorge Rencoret,Ana Gutiérrez,Hui Sun,Erika Lindquist,Kerrie Barry,Robert Riley,Igor V. Grigoriev,Bernard Henrissat,Ursula Kües,Randy M. Berka,Ángel T. Martínez,Sarah F. Covert,Robert A. Blanchette,Daniel Cullen +40 more
TL;DR: The annotated P. gigantea genome is reported and profiles of its transcriptome and secretome when cultured on fresh-cut versus solvent-extracted loblolly pine wood provide insight into the diverse chemistries employed by fungi in carbon cycling processes.
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