Journal Article10.1038/NNANO.2009.202
A novel magnetic crystal-lipid nanostructure for magnetically guided in vivo gene delivery.
Yoshihisa Namiki,Tamami Namiki,Hiroshi Yoshida,Yukiko Ishii,Akihito Tsubota,Shigeo Koido,Kouichi Nariai,Makoto Mitsunaga,Satoru Yanagisawa,Hideyuki Kashiwagi,Yasuo Mabashi,Yoko Yumoto,Sadayori Hoshina,Kiyotaka Fujise,Norio Tada +14 more
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TL;DR: It is shown that a new nanoparticle formulation can be magnetically guided to deliver and silence genes in cells and tumours in mice, and this formulation, termed LipoMag, consists of an oleic acid-coated magnetic nanocrystal core and a cationic lipid shell.
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Abstract: Cancer gene therapy requires a safe and effective gene delivery system. Polymer- and lipid-coated magnetic nanocrystals have been used to deliver silencing RNA, but synthesizing these magnetic vectors is difficult. Here, we show that a new nanoparticle formulation can be magnetically guided to deliver and silence genes in cells and tumours in mice. This formulation, termed LipoMag, consists of an oleic acid-coated magnetic nanocrystal core and a cationic lipid shell. When compared with the commercially available PolyMag formulation, LipoMag displayed more efficient gene silencing in 9 of 13 cell lines, and better anti-tumour effects when systemically administered to mice bearing gastric tumours. By delivering an optimized sequence of a silencing RNA that targets the epidermal growth factor receptor of tumour vessels, the intended therapeutic benefit was achieved with no evident adverse immune reaction or untoward side effects.
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Superparamagnetic nanoparticles for biomedical applications: Possibilities and limitations of a new drug delivery system
TL;DR: The characteristics and applications of SPION in the biomedical sector are introduced and discussed, and superparamagnetic nanoparticles based on a core consisting of iron oxides that can be targeted through external magnets are discussed.
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•Journal Article
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
TL;DR: It is shown that chemically modified short interfering RNAs (siRNAs) can silence an endogenous gene encoding apolipoprotein B (apoB) after intravenous injection in mice, and it is determined that cleavage of the apoB mRNA occurred specifically at the predicted site.
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Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo.
Franz Scherer,Martina Anton,Ulrike Schillinger,Julia Henke,C Bergemann,Achim Krüger,Bernd Gansbacher,Christian Plank +7 more
TL;DR: In this paper, the authors associated gene vectors with superparamagnetic nanoparticles and targeted gene delivery by application of a magnetic field, which potentiated the efficacy of any vector up to several hundred-fold, allowed reduction of the duration of gene delivery to minutes, extended the host tropism of adenoviral vectors to nonpermissive cells and compensated for low retroviral titer.
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•Journal Article
Photochemical internalization: a novel technology for delivery of macromolecules into cytosol.
Kristian Berg,Pål Kristian Selbo,Lina Prasmickaite,Torunn Elisabeth Tjelle,Kirsten Sandvig,Johan Emelian Moan,Gustav Gaudernack,Øystein Fodstad,Siv Kjølsrud,Helle Anholt,Gry Hege Rodal,Siv Kjersti Rodal,Anders Høgset +12 more
TL;DR: Results presented here show that PCI can induce efficient light-directed delivery of macromolecules into the cytosol, indicating that PCI may have a variety of useful applications for site-specific drug delivery, e.g., in gene therapy, vaccination, and cancer treatment.
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The magnetofection method: using magnetic force to enhance gene delivery.
Christian Plank,Ulrike Schillinger,Franz Scherer,Christian Bergemann,Jean-Serge Remy,Florian Krötz,Martina Anton,Jim Lausier,Joseph Rosenecker +8 more
Abstract: In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles. Here we review the benefits, the mechanism and the potential of the method with regard to overcoming physical limitations to gene delivery. Magnetic particle chemistry and physics are discussed, followed by a detailed presentation of vector formulation and optimization work. While magnetofection does not necessarily improve the overall performance of any given standard gene transfer method in vitro, its major potential lies in the extraordinarily rapid and efficient transfection at low vector doses and the possibility of remotely controlled vector targeting in vivo.