A new generation of the IMAGIC image processing system.

TL;DR: Hydrodynamic analyses of NSF mutants, with specific functional defects, define the structures underlying these conformational changes and suggest a mechanism for NSF binding to and disassembly of SNARE complexes.

TL;DR: The solution structure of the Thermus thermophilus GroEL-GroES complex encapsulating its substrate proteins was determined by use of cryo-electron microscopy and a distortion of the cis-GroEL ring indicated that the interaction between the encapsulated proteins and the GroEL ring results in an adjustment in the cavity's shape to accommodate the substrate.

TL;DR: It is shown through sRNA mutagenesis, native gel electrophoresis, and single-particle EM that a di-sRNP is the near exclusive architecture obtained when reconstituting box C/D sRNPs with natural or artificial sRNAs containing an internal loop.

TL;DR: The architecture and implementation of VoxeLine, a new interactive environment for display and analysis of 2D and 3D images in real-time, is discussed and its ability to show datasets in all directions without duplicating data into the main memory is discussed.

TL;DR: General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images and applied to the calculation of aThree dimensional density map of the tail of bacteriophage T4.

TL;DR: In this article, a method of "angular reconstitution" is described, which allows the a posteriori determination of the relative angular orientations of the projections and thus enables the 3D reconstruction of the object to be calculated.

TL;DR: The authors decrit une approche heuristique pour calculer les fonctions filtres qui sont exactes pour chaque projection dans toute geometrie de reconstruction donnee.