A new generation of the IMAGIC image processing system.

TL;DR: Bioinformatics analysis of currently predicted GroEL-like proteins encoded by phage genomes in comparison with cellular and mitochondrial chaperonins revealed the different conformational states of OBP chaper onin, depending on the bound nucleotide.

TL;DR: A 3D reconstruction of the 1 x 6-mer hemocyanin from the European spiny lobster Palinurus elephas has been performed from 9970 single particles using cryoelectron microscopy, demonstrating the reliability of both methods for reconstructing proteins.

TL;DR: A sequence-specific RNA binding protein was fused to a protein with a distinct donut-shaped structure to label spliceosomes assembled on a pre-mRNA that contained the target sequence in the exons, which will serve as a general tool for analyzing the structures of RNA-containing macromolecular complexes.

TL;DR: Several chromatin remodellers have the ability to space nucleosomes on DNA but this involves an interplay between H4 histone tails, the AutoN and NegC motifs of the motor domains that together regulate ATPase activity and sense the length of DNA flanking the nucleosome, by contrast with the INO80 complex.

TL;DR: General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images and applied to the calculation of aThree dimensional density map of the tail of bacteriophage T4.

TL;DR: In this article, a method of "angular reconstitution" is described, which allows the a posteriori determination of the relative angular orientations of the projections and thus enables the 3D reconstruction of the object to be calculated.

TL;DR: The authors decrit une approche heuristique pour calculer les fonctions filtres qui sont exactes pour chaque projection dans toute geometrie de reconstruction donnee.