Journal Article10.1016/J.MIMET.2007.08.007
A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes.
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TL;DR: The use of the multiplex PCR targeting inlA, inlC and inlJ genes facilitates simultaneous confirmation of L. monocytogenes species identity and virulence.
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About: This article is published in Journal of Microbiological Methods. The article was published on 01 Nov 2007. The article focuses on the topics: Internalin & Virulence.
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Citations
Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotyping
TL;DR: This molecular serotyping scheme could be considered as a useful and rapid method for first-level characterisation of the most frequently encountered L. monocytogenes serotypes.
158
A review of Listeria monocytogenes from meat and meat products: Epidemiology, virulence factors, antimicrobial resistance and diagnosis.
TL;DR: Information on prevalence and control measures of L. monocytogenes along the meat value chain is provided and the pathogenicity, virulence traits and antimicrobial resistance profiles of this zoonotic pathogen are discussed.
Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets
Hossein Jamali,Mohammadjavad Paydar,Salmah Ismail,Chung Yeng Looi,Won Fen Wong,Behrad Radmehr,Atefeh Abedini +6 more
TL;DR: Recovery of potentially pathogenic L. monocytogenes from raw fish and environment of open-air fish market samples in this study is a convincing evidence for the zoonotic potential of listeriosis.
Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China.
TL;DR: The presence of virulence markers, epidemic clones, as well as the antibiotic resistance amongst the isolates strongly implies that many of these strains might be capable of causing listeria, and more accurate treatment of human listeriosis with effective antibiotics should be considered.
An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
TL;DR: Overall, molecular methods for cultural detection, enumeration, and molecular identification of L. monocytogenes are rapid, sensitive, specific, time- and labor-saving and there are chances for the development of new techniques for the detection and identification of foodborne with improved features.
References
Assessment of the virulence of Listeria monocytogenes: agreement between a plaque-forming assay with HT-29 cells and infection of immunocompetent mice
Sylvie M. Roche,Philippe Velge,Elisabeth Bottreau,Christine Durier,Nathalie Marquet-van der Mee,P. Pardon +5 more
TL;DR: A new in vitro test of virulence based on a plaque-forming assay (PFA) using a HT-29 cell monolayer with 118 Listeria strains was validated, demonstrating the reproducibility and repeatability of the PFA and good agreement was observed between virulence assessed after subcutaneous (SC) infection and virulence obtained by PFA.
Select Listeria monocytogenes subtypes commonly found in foods carry distinct nonsense mutations in inlA, leading to expression of truncated and secreted internalin A, and are associated with a reduced invasion phenotype for human intestinal epithelial cells.
TL;DR: Screening of L. monocytogenes strains isolated from human clinical cases, foods, and healthy animals in the United States revealed mutations in inlA leading to premature stop codons (PMSCs) that are associated with, and possibly at least partially responsible for, an attenuated invasion phenotype.
Listeria monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features.
Dongyou Liu,Mark L. Lawrence,Lisa Gorski,Robert E. Mandrell,A.Jerald Ainsworth,Frank W. Austin +5 more
TL;DR: Findings indicate that L. monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features.