A method for determining enzymatically hydrolyzable phosphate (EHP) in natural waters1
TL;DR: The new method is simple, accurate, and can be used in both freshwater and marine studies, and is particularly recommended for determination in hypereutrophic waters, when Pi concentrations exceed 25 µg P liter−1.
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Abstract: A method for determining enzymatically hydrolyzable phosphate (EHP) in natural waters is described, based on the determination of released inorganic phosphate after the hydrolysis of organophosphoric esters by free, dissolved phosphohydrolases (mainly phosphatase) produced by the biota. The method gives higher values in highly eutrophic waters than the classical procedure of Strickland and Parsons; in samples from less eutrophic habitats the two methods give similar results. The new method is simple, accurate, and can be used in both freshwater and marine studies. It is particularly recommended for determination in hypereutrophic waters, when Pi concentrations exceed 25 µg P liter−1. Representative field data from the euphotic zone of seven lakes and two marine habitats are presented.
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References
•Book
A practical handbook of seawater analysis
J. D. H. Strickland,Timothy R. Parsons +1 more
- 01 Jan 1968
11.8K
Methods of seawater analysis
Klaus Grasshoff,Klaus Kremling,Manfred Ehrhardt +2 more
- 27 Jan 1999
TL;DR: The Automatic Determination of Dissolved Organic Carbon (DOC) by Wet Chemical Oxidation is described in this paper, along with the results of HPLC analysis of photosynthetic pigments.
8.4K
The relative importance of bacterioplankton and phytoplankton in phosphorus uptake in freshwater1
David J. Currie,Jaap Kalff +1 more
TL;DR: The bacterioplankton apparently strongly dominates orthophosphate cycling in situ, while the phytoplANKton seems to obtain most of its phosphorus from excreted organic P compounds.
254
Free Dissolved Enzymes in Lake Waters
TL;DR: In this paper, the authors investigated free phosphatases in the waters of eutrophic lakes, including Pluss-See near Ploen, with special reference to their ecological importance.
172