Journal Article10.1021/JA404649M
A highly specific fluorescent probe for hypochlorous acid and its application in imaging microbe-induced HOCl production.
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TL;DR: FBS is designed as a new fluorescent HOCl probe which has high selectivity, sensitivity, and short response time in a broad range of pH, and the "dual-lock" structure of FBS has an advantage of eliminating interferences from other ROS/RNS.
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Abstract: Oxidative stress induced by reactive oxygen species (ROS) plays crucial roles in a wide range of physiological processes and is also implicated in various diseases, including cancer, chronic inflammatory diseases, and neurodegenerative disorders. Among the various ROS, hypochlorous acid (HOCl) plays as a powerful microbicidal agent in the innate immune system. The regulated production of microbicidal HOCl is required for the host to control the invading microbes. However, as a result of the highly reactive and diffusible nature of HOCl, its uncontrolled production may lead to an adverse effect on host physiology. Because of its biological importance, many efforts have been focused on developing selective fluorescent probes to image ROS. However, it is still challenging to design a fluorescent probe with exclusive selectivity toward a particular member of ROS. In the current work, we designed FBS as a new fluorescent HOCl probe which has high selectivity, sensitivity, and short response time in a broad ran...
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Citations
A bifunctional fluorescent probe for dual-channel detection of H2O2 and HOCl in living cells
Qingguo He,Shunping Zang,Yuyang Zeng,Benhua Wang,Xiangzhi Song +4 more
Abstract: Hydrogen peroxide (H2O2) and hypochlorous acid (HOCl) are critical reactive oxygen species (ROS) that play significant roles in regulating oxidative stress, closely tied to various human diseases. However, investigating their interplay within living cells has been challenging due to the lack of effective tools for simultaneous discrimination. Herein, we present a bifunctional fluorescent probe, PTZ-H-H, capable of simultaneously detecting H2O2 and HOCl in living cells via two distinct fluorescence channels. PTZ-H-H exhibits selective and sensitive responses, emitting red fluorescence in the presence of H2O2 and green fluorescence in response to HOCl, with detection limits of 386 nM and 16.8 nM, respectively. The probe was successfully applied in living cells, enabling real-time monitoring of intracellular H2O2 and HOCl. This study demonstrates the potential of PTZ-H-H as a powerful tool for exploring the dynamic roles of H2O2 and HOCl in various physiological and pathological processes.
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Carboxy Bodipy-Based Fast Trigger Fluorescent Probe for Imaging Endogenous Hypochlorous Acid
TL;DR: In this article , a carboxy Bodipy-based probe was developed for rapid, sensitive, and specific monitoring of hypochlorous acid (HClO/ClO−) in cells.
Rational design of an HClO-specific triggered self-immolative fluorescent turn-on sensor and its bioimaging applications.
Xiao-Bo Wang,Da-Long Zhang,Hui-Jing Li,Xuhao Lu,Quanze Liu,Yan-Chao Wu +5 more
TL;DR: The design and evaluation of an HClO-specific triggered self-immolative fluorescent sensor (RESClO) based on the structure of an N-protected Resorufin dye, which was successfully used for bioimaging of exogenous and endogenous HclO in cells and zebra fish and can also be used for visual imaging of mouse arthritis models.
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Double-mode detection of HClO by naked eye and concurrent fluorescence increasing in absolute PBS
TL;DR: In this paper, a water soluble fluorescent probe WCN was successfully designed and synthesized based on acenaphthenequinone, which can be employed for doublemode detection of HClO by the naked eye and concurrent significant fluorescence increasing in absolute PBS.
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Progress and Perspective of Atherosclerosis-Targeted Molecular Probes: From Precise Imaging to Auxiliary Diagnosis and Treatment
Cong Hu,Zhipengjun Zhang,Shanyi Sun,Hong-Wen Liu,Lin Yuan,Xiaobin Zhang +5 more
TL;DR: This review discusses the development of molecular probes for atherosclerosis imaging, diagnosis, and treatment, highlighting their potential for non-invasive detection and precise identification of disease sites, and emphasizing challenges and prospects in the field.
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