Journal Article10.1021/JA404649M
A highly specific fluorescent probe for hypochlorous acid and its application in imaging microbe-induced HOCl production.
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TL;DR: FBS is designed as a new fluorescent HOCl probe which has high selectivity, sensitivity, and short response time in a broad range of pH, and the "dual-lock" structure of FBS has an advantage of eliminating interferences from other ROS/RNS.
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Abstract: Oxidative stress induced by reactive oxygen species (ROS) plays crucial roles in a wide range of physiological processes and is also implicated in various diseases, including cancer, chronic inflammatory diseases, and neurodegenerative disorders. Among the various ROS, hypochlorous acid (HOCl) plays as a powerful microbicidal agent in the innate immune system. The regulated production of microbicidal HOCl is required for the host to control the invading microbes. However, as a result of the highly reactive and diffusible nature of HOCl, its uncontrolled production may lead to an adverse effect on host physiology. Because of its biological importance, many efforts have been focused on developing selective fluorescent probes to image ROS. However, it is still challenging to design a fluorescent probe with exclusive selectivity toward a particular member of ROS. In the current work, we designed FBS as a new fluorescent HOCl probe which has high selectivity, sensitivity, and short response time in a broad ran...
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Citations
Enhanced fluorescence sensing of hypochlorous acid using serum albumin as a signal amplifier.
TL;DR: In this paper, a mitochondria-targeting fluorescent probe was developed based on oxidation by HOCl/ClO−, releasing a green emissive luminogen under physiological conditions.
22
A pH-sensitive multifunctional fluorescent probe based on N-annulated perylene for the sensitive and selective detection of hypochlorous acid
TL;DR: In this paper, Nannulated perylene (NP) was chosen as the fluorophore to design a multifunctional fluorescent probe for the sensitive and selective detection of HOCl for the first time.
21
A Sensitive and Selective Fluorescent Coumarin-Based Probe for Detection of Hypochlorite ion and its Application to Cellular Imaging
TL;DR: A readily available turn-off fluorescent probe for ClO− is reported, which displays highly selectivity and sensitivity over other common anions and reactive oxygen/nitrogen.
21
DNA-encapsulated silver nanodots as ratiometric luminescent probes for hypochlorite detection
TL;DR: A new ratiometric luminescence imaging method based on silver nanodots to sensitively detect hypochlorite is introduced and the factors that influence the accuracy of the detection are investigated.
Red-emitting fluorescent probe for selective and sensitive determination hypochlorite in living cells
TL;DR: In this article, the water-soluble red-emitting fluorescent probe (probe 1) was used for sensing ClO− in living cells, which has excellent sensitivity with the limit of detection (LOD) of 2.41m.
21
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