A high-throughput, field-based phenotyping technology for tall biomass crops
TL;DR: Phenobot 1.0, an auto-steered and self-propelled field-based high-throughput phenotyping platform for tall dense canopy crops, such as sorghum, was developed and tested and was proven robust to obtain ground-basedHigh-Throughput plant architecture parameters of sorghums, a tall and densely planted crop species.
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Abstract: Recent advances in omics technologies have not been accompanied by equally efficient, cost-effective, and accurate phenotyping methods required to dissect the genetic architecture of complex traits. Even though high-throughput phenotyping platforms have been developed for controlled environments, field-based aerial and ground technologies have only been designed and deployed for short-stature crops. Therefore, we developed and tested Phenobot 1.0, an auto-steered and self-propelled field-based high-throughput phenotyping platform for tall dense canopy crops, such as sorghum (Sorghum bicolor). Phenobot 1.0 was equipped with laterally positioned and vertically stacked stereo RGB cameras. Images collected from 307 diverse sorghum lines were reconstructed in 3D for feature extraction. User interfaces were developed, and multiple algorithms were evaluated for their accuracy in estimating plant height and stem diameter. Tested feature extraction methods included the following: (1) User-interactive Individual Plant Height Extraction (UsIn-PHe) based on dense stereo three-dimensional reconstruction; (2) Automatic Hedge-based Plant Height Extraction (Auto-PHe) based on dense stereo 3D reconstruction; (3) User-interactive Dense Stereo Matching Stem Diameter Extraction; and (4) User-interactive Image Patch Stereo Matching Stem Diameter Extraction (IPaS-Di). Comparative genome-wide association analysis and ground-truth validation demonstrated that both UsIn-PHe and Auto-PHe were accurate methods to estimate plant height, while Auto-PHe had the additional advantage of being a completely automated process. For stem diameter, IPaS-Di generated the most accurate estimates of this biomass-related architectural trait. In summary, our technology was proven robust to obtain ground-based high-throughput plant architecture parameters of sorghum, a tall and densely planted crop species.
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Citations
Crop Phenomics and High-Throughput Phenotyping: Past Decades, Current Challenges, and Future Perspectives.
Wanneng Yang,Hui Feng,Xuehai Zhang,Jian Zhang,John H. Doonan,William D. Batchelor,Lizhong Xiong,Jianbing Yan +7 more
TL;DR: Main developments on high-throughput phenotyping in the controlled environments and field conditions as well as for post-harvest yield and quality assessment in past decades are reviewed and the latest multiomics works combining high- throughput phenotypesing and genetic studies are described.
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Estimate of winter-wheat above-ground biomass based on UAV ultrahigh-ground-resolution image textures and vegetation indices
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TL;DR: In this paper, the authors evaluated the use of image textures, VIs, and combinations thereof to make multiple temporal estimates and maps of AGB covering three winter-wheat growth stages.
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High throughput determination of plant height, ground cover, and above-ground biomass in wheat with LiDAR
José A. Jiménez-Berni,José A. Jiménez-Berni,David M. Deery,Pablo Rozas-Larraondo,Anthony G. Condon,Anthony G. Condon,Greg J. Rebetzke,Richard A. James,William D. Bovill,Robert T. Furbank,Robert T. Furbank,Xavier Sirault,Xavier Sirault +12 more
TL;DR: Application of this system will provide new opportunities to deliver improved genotypes and agronomic interventions via more efficient and reliable phenotyping of these important traits in large experiments through rapid multi-temporal and non-destructive estimation of canopy height, ground cover and above-ground biomass.
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A Physio-Morphological Trait-Based Approach for Breeding Drought Tolerant Wheat.
TL;DR: There is a need for comprehensive high throughput phenotyping of physio-morphological traits that is growth stage-based to improve the efficiency of breeding drought-tolerant wheat.
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