Journal Article10.1007/BF00019559
A gene for Brassica napus extensin is differentially expressed on wounding
28
TL;DR: Application of the wounding stimulus results in the accumulation of extensin gene transcripts to different degrees and at different times in the aerial parts of the plant, and results in a decline in the same transcripts in the roots.
read more
Abstract: We have analysed the expression of the endogenous extensin genes in Brassica napus, using northern hybridisation and dot blotting. In the unstressed plant, the extA gene is only expressed in the root, expression in the leaf, petiole and stem being absent. We have found that wounding dramatically alters this normal pattern of expression. Expression in wounded leaf is seen after 36 h, in wounded petioles after 11 h and in wounded stem after 17 h. Differences in the amount of extensin mRNA accumulated are also seen: wounded petiole accumulating extensin message to a level higher than the leaf or the stem. Inhibitors of ethylene biosynthesis greatly delay the onset of accumulation of extensin mRNA in wounded tissues. Wounding the root causes the level of extensin message to decline with time, until levels below the limit of non-specific hybridisation are reached 11 h after wounding. Thus, application of the wounding stimulus results in the accumulation of extensin gene transcripts to different degrees and at different times in the aerial parts of the plant, and results in a decline in the same transcripts in the roots. Extensin transcript accumulation as a result of wounding is also dependent on the age of the tissue; high levels of message are seen in old wounded leaves, while expression in young wounded leaves is absent.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Role of proline in cell wall synthesis and plant development and its implications in plant ontogeny.
Polavarapu B. Kavi Kishor,P. Hima Kumari,M. S. L. Sunita,Nese Sreenivasulu,Nese Sreenivasulu +4 more
TL;DR: The role of extensins, arabinogalactan proteins and hydroxyproline- and proline-rich proteins as important components of cell wall proteins that play pivotal roles in cell wall signal transduction cascades, plant development and stress tolerance is discussed in this review.
Shaping in plant cells.
TL;DR: Although turgor has long been assumed to be a rather passive contributor to cell shaping, recent reports show that, in some cells, differential changes inTurgor may have a role in establishing specialised cell form.
125
The proline-rich, extensin-like receptor kinase-1 (PERK1) gene is rapidly induced by wounding
Nancy F. Silva,Daphne R. Goring +1 more
TL;DR: The isolation and characterization of PERK1 (Proline Extensin-like Receptor Kinase 1), a novel plant RLK from Brassica napusthat is predicted to consist of a proline-rich extracellular domain with sequence similarity to extensins, a transmembrane region, and a catalytic domain possessing serine/threonine kinase activity is reported.
106
Rapid deposition of extensin during the elicitation of grapevine callus cultures is specifically catalyzed by a 40-kilodalton peroxidase.
Phil A. Jackson,Carla I. R. Galinha,Cristina Silva Pereira,Ana Sofia Fortunato,Nelson C. Soares,Sara Amancio,Candido P.P. Ricardo +6 more
TL;DR: This work concludes that it has identified and purified an EP from grapevine callus that is responsible for the catalysis of GvP1 deposition in situ during elicitation and suggests that GVP1 and this EP play an important combined role in grapevine cell wall defense.
101
Gene expression in leaves of Theobroma cacao in response to mechanical wounding, ethylene, and/or methyl jasmonate
TL;DR: The cacao response to wounding, ethylene, and/or methyl jasmonate was influenced by developmental stage, and cross-talk between ethylene and methyl jAsmonate action on cacao gene expression resulted in synergistic and antagonistic responses.
82
References
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
26.1K
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Andrew P. Feinberg
- 01 Jan 1984
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
22.6K
Tissue-Specific Expression of Cell Wall Proteins in Developing Soybean Tissues.
Zheng-Hua Ye,Joseph E. Varner +1 more
TL;DR: Results show that the gene expression of HRGPs and GRPs is developmentally regulated in a tissue-specific manner, and that GRPs are specifically localized in primary xylem cell walls of young root.
Hydroxyproline- O -glycosidic Linkage of the Plant Cell Wall Glycoprotein Extensin
TL;DR: The recent isolation of several glycopeptides, rich in hydroxyproline from enzymatic digests of tomato cell walls, confirmed the inference of a covalent carbohydrate–protein linkage in extensin, and led to the preliminary conclusion that attachment of the carbohydrate is by a glycosidic link through the hydroxyl group of hydroxy Proline.
233
Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants.
TL;DR: Three different transcripts induced by fungal elicitor, wounding, or infection which encode apoproteins of cell wall hydroxyproline-rich glycoproteins involved in plant defense against infection are characterized, indicating the operation of several distinct intercellular stress signal systems in higher plants.
225