A Fully Automated Robotic System for Microinjection of Zebrafish Embryos
TL;DR: A microrobotic system for fully automated zebrafish embryo injection is presented, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility.
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Abstract: As an important embodiment of biomanipulation, injection of foreign materials (e.g., DNA, RNAi, sperm, protein, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This paper presents a microrobotic system for fully automated zebrafish embryo injection, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility. Based on computer vision and motion control, the microrobotic system performs injection at a speed of 15 zebrafish embryos (chorion unremoved) per minute, with a survival rate of 98% (n = 350 embryos), a success rate of 99% (n = 350 embryos), and a phenotypic rate of 98.5% (n = 210 embryos). The sample immobilization technique and microrobotic control method are applicable to other biological injection applications such as the injection of mouse oocytes/embryos and Drosophila embryos to enable high-throughput biological and pharmaceutical research.
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Citations
Establishment of an integrated automated embryonic manipulation system for producing genetically modified mice.
Tomoo Eto,Hiroki Ueda,Ryoji Ito,Tsukasa Takahashi,Toshiaki Watanabe,Motohito Goto,Yusuke Sotomaru,Nobuaki Tanaka,Ri-ichi Takahashi +8 more
TL;DR: The Integrated Automated Embryo Manipulation System (IAEMS) as mentioned in this paper is a fully automated system for microinjection into the pronucleus of a zygote to facilitate genetic modification.
A Vision-guided Methodology for the Automation of Biological Cell Injection
Ferhat Sadak,Mozafar Saadat,Amir M. Hajiyavand +2 more
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TL;DR: In this article, a vision-based technique was proposed for the automation of microinjection applications, which can detect the injection and holding pipette as a part of the injection system and then automatically align them in three orthogonal axes.
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