A Fully Automated Robotic System for Microinjection of Zebrafish Embryos
TL;DR: A microrobotic system for fully automated zebrafish embryo injection is presented, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility.
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Abstract: As an important embodiment of biomanipulation, injection of foreign materials (e.g., DNA, RNAi, sperm, protein, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This paper presents a microrobotic system for fully automated zebrafish embryo injection, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility. Based on computer vision and motion control, the microrobotic system performs injection at a speed of 15 zebrafish embryos (chorion unremoved) per minute, with a survival rate of 98% (n = 350 embryos), a success rate of 99% (n = 350 embryos), and a phenotypic rate of 98.5% (n = 210 embryos). The sample immobilization technique and microrobotic control method are applicable to other biological injection applications such as the injection of mouse oocytes/embryos and Drosophila embryos to enable high-throughput biological and pharmaceutical research.
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Citations
Fabrication of Spring Steel and PDMS Grippers for the Micromanipulation of Biological Cells
G. K. Ananthasuresh,Nandan Maheswari,A. Narayana Reddy,Deepak Sahu +3 more
- 01 Jan 2010
TL;DR: In this article, the authors describe a minimally intrusive force-sensing technique that does not need any specialized sensor other than the gripper itself to estimate the forces exerted on the cell.
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Accurate nano-injection system for capillary gas chromatography.
TL;DR: Good repeatability of the calibration curves for the hydrocarbons ethanolic solution confirmed its feasibility in quantitative analysis regardless of concentration, and suggested that it can be an accurate nano-injector for cGC.
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Centroid calculation of the blastomere from 3D Z-Stack image data of a 2-cell mouse embryo
TL;DR: The proposed calculation of the blastomere centroid is a critical step in determining the centroid of objects within a cell, to facilitate automation of cell surgery tasks.
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From teleoperated to automatic blastocyst microinjections: Designing a new system from expert-controlled operations
Leonardo S. Mattos,Edward Grant,Randy J. Thresher,K. Kluckman +3 more
- 14 Oct 2008
TL;DR: The goal of increasing the consistency and efficiency rates of these operations was pursued through robotics and automation, and the validity of the derived rules for automatic blastocyst microinjections was attested by demonstrating a 75% success rate.
11
Macro-to-micro positioning and auto focusing for fully automated single cell microinjection
TL;DR: Algorithms and methods for the macro-to-micro positioning and auto focusing of the microgripper jaws with the success rate 100% and 90%, respectively are presented.
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