A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications
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TL;DR: The applicability of a thermomorphic multiphasic system composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated in this article .
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Abstract: Abstract The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine‐based DES with the fatty acid oleic acid as a hydrogen‐bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES‐enriched layer. Finally, a reduction catalyzed by horse liver alcohol dehydrogenase was performed in a larger‐scale experiment, and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
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A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications
TL;DR: The applicability of a thermomorphic multiphasic system composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated in this article .
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References
Novel solvent properties of choline chloride/urea mixtures
TL;DR: Eutectic mixtures of urea and a range of quaternary ammonium salts are liquid at ambient temperatures and have interesting solvent properties.
4.6K
Industrial biocatalysis today and tomorrow
TL;DR: Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells.
2.3K
Natural Deep Eutectic Solvents – Solvents for the 21st Century
Alexandre Paiva,Rita Craveiro,Ivo Manuel Ascensão Aroso,Marta Martins,Rui L. Reis,Ana Rita C. Duarte +5 more
TL;DR: Deep Eutectic Solvents (DES) as discussed by the authors are a class of solvents that can be defined as a mixture of two or more components, which at a particular composition present a high melting point depression becoming liquids at room temperature.
1.9K
Green solvents for sustainable organic synthesis: state of the art
TL;DR: A review of the state of the art in the use of alternative reaction media for green, sustainable organic synthesis is presented in this article, where a novel and effective method for the immobilisation of enzymes as cross-linked enzyme aggregates (CLEAs) is discussed and a combi CLEA, containing two enzymes, for the one-pot conversion of benzaldehyde to S-mandelic acid is reported.
1.5K
Tools and techniques for solvent selection: green solvent selection guides
Fergal Byrne,Saimeng Jin,Giulia Paggiola,Tabitha H. M. Petchey,James H. Clark,Thomas J. Farmer,Andrew J. Hunt,C. Robert McElroy,James Sherwood +8 more
- 23 May 2016
TL;DR: A review of general purpose solvent selection guides can be found in this paper, highlighting their similarities and differences and how they can be used to enhance the greenness of chemical processes, particularly in laboratory organic synthesis and the pharmaceutical industry.