Journal Article10.1046/J.1365-2958.1997.2311591.X
A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K‐12
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TL;DR: Cloned a 35.4 kb ‘pathogenicity island’ from the prototype AE bacterium, enteropathogenic Escherichia coli, containing all previously described AE genes, demonstrating that the defining feature of this class of pathogens can be acquired by an avirulent bacterium in a single genetic step.
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Abstract: Attaching and effacing (AE) bacteria are a diverse group of gastrointestinal pathogens, comprising members of four genera, that cause the intestinal epithelial microvilli to be replaced with raised clusters of filamentous actin that conform to the surface of attached bacteria. We have cloned a 35.4 kb ‘pathogenicity island’ from the prototype AE bacterium, enteropathogenic Escherichia coli, containing all previously described AE genes. Transfer of this pathogenicity island to avirulent E. coli converts the recipients into strains that secrete virulence proteins, induce host signal-transduction pathways, and cause AE lesions on cultured epithelial cells. These results demonstrate that this pathogenicity island contains all pathogen-specific genes necessary for inducing AE lesions, and that the defining feature of this class of pathogens can be acquired by an avirulent bacterium in a single genetic step.
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Citations
A third secreted protein that is encoded by the enteropathogenic Escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells.
TL;DR: A novel locus encoding a secreted protein that is required for attaching and effacing activity is described, and it is demonstrated by immunoblotting and microsequencing that the EspD protein is secreted via the type III apparatus.
Self-association of EPEC intimin mediated by the β-barrel-containing anchor domain: a role in clustering of the Tir receptor
TL;DR: Modelling suggests that actin rearrangement induced in Tir‐primed cultured cells by intimin‐containing proteoliposomes was dependent on the concentration of both intimin and Tir, and co‐localized with clustered phosphorylated Tir.
Use of comparative genomics as a tool to assess the clinical and public health significance of emerging Shiga toxin-producing Escherichia coli serotypes
TL;DR: The clinico-pathological features and pathogenesis of STEC infection, new information arising from E. coli O157:H7 genome sequences, and progress in the use of comparative genomics for assessing potential differences in virulence and public health significance between STEC subgroups are highlighted.
•Dissertation
Genomic mosaicism in the opportunistic pathogen Pseudomonas aeruginosa and its contribution to virulence
Ewan Michael Harrison
- 01 Jan 2010
TL;DR: The results demonstrated that rrn operon-mediated inversions are a constantly occurring in P. aeruginosa populations and identified a potential link between the number of chromosomally-integrated copies o f PAPI-1 and the stability of the island.
Citrobacter rodentium, the causative agent of transmissible murine colonic hyperplasia, exhibits clonality: synonymy of C. rodentium and mouse-pathogenic Escherichia coli.
Steven A. Luperchio,Joseph V. Newman,Charles A. Dangler,Mark D. Schrenzel,Don J. Brenner,Arnold G. Steigerwalt,David B. Schauer +6 more
TL;DR: It is demonstrated that MPEC is a misclassified C. rodentium isolate and that members of this species are clonal and represent the only known attaching and effacing bacterial pathogen of mice.
References
A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.
TL;DR: It is reported that in EPEC a 35-kbp locus containing several regions implicated in formation of these lesions is found, which hybridize to E. coli O157:H7 and other pathogens of three genera that cause similar lesions but do not hybridized to avirulent members of the same species.
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A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.
TL;DR: The ability of enteropathogenic Escherichia coli to form attaching and effacing intestinal lesions is a major characteristic of EPEC pathogenesis and a chromosomal gene (eae) that is necessary for this activity is identified using TnphoA mutagenesis.
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Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines.
TL;DR: Three strains of enteropathogenic Escherichia coli (EPEC), originally isolated from humans and previously shown to cause diarrhea in human volunteers by unknown mechanisms, and one rabbit EPEC strain were shown to attach intimately to and efface microvilli and cytoplasm from intestinal epithelial cells in both the pig and rabbit intestine.
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Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation
Karen G. Jarvis,Jorge A. Girón,Ann E. Jerse,Timothy K. McDaniel,Michael S. Donnenberg,James B. Kaper +5 more
TL;DR: The identified EPEC chromosomal genes whose predicted protein sequences are similar to components of a recently described secretory pathway (type III) responsible for exporting proteins lacking a typical signal sequence suggest that the EPEC Sep proteins are component of a type III secretory apparatus necessary for the export of virulence determinants.
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Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins
TL;DR: Results indicate that attachment of H. pylori to gastric epithelial cells resembles that of enteropathogenic Escherichia coli, and cytoskeletal components actin, alpha-actinin, and talin are involved in the process.
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