A catalogue of splice junction sequences
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TL;DR: The sequence CAAG/GTAGAGT was found to be a consensus of 139 exon-intron boundaries (or donor sequences) and (TC)nNCTAG/G was found of 130 intron-exon boundaries ( or acceptor sequences).
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Abstract: Splice junction sequences from a large number of nuclear and viral genes encoding protein have been collected. The sequence CAAG/GTAGAGT was found to be a consensus of 139 exon-intron boundaries (or donor sequences) and (TC)nNCTAG/G was found to be a consensus of 130 intron-exon boundaries (or acceptor sequences). The possible role of splice junction sequences as signals for processing is discussed.
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References
Are snRNPs involved in splicing
TL;DR: Several lines of evidence are presented that suggest a direct involvement of snRNPs in the splicing of hnRNA molecules, including the observation that the nucleotide sequence at the 5′ end of U1 RNA exhibits extensive complementarity to those across splice junctions in hn RNA molecules.
1.2K
Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
TL;DR: Selected regions of cloned EcoRI fragments of the chicken ovalbumin gene have been sequenced, and a previously unreported interruption in the DNA sequences coding for the 5' nontranslated region of the messenger has been discovered.
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Sequence of the human insulin gene
Graeme I. Bell,Raymond Pictet,William J. Rutter,Barbara Cordell,Edmund Tischer,Howard M. Goodman +5 more
TL;DR: A comparison of the human with the rat insulin genes indicates potential regulatory regions in the DNA segment preceding the gene and suggests that the ancestral form of the insulin gene had two intervening sequences.
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Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments.
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