Book Chapter10.1016/S0076-6879(83)91052-2
[50] Protein labeling by reductive alkylation
Neil Jentoft,Dorr G. Dearborn +1 more
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About: This article is published in Methods in Enzymology. The article was published on 01 Jan 1983. The article focuses on the topics: Alkylation & Methylation.
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Citations
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References
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Sidney Udenfriend,Stanley Stein,Peter Böhlen,Wallace Dairman,Willy Leimgruber,Manfred Weigele +5 more
TL;DR: Fluorescamine is a new reagent for the detection of primary amines in the picomole range that is almost instantaneous at room temperature in aqueous media and the products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent.
2.6K
Cyanohydridoborate anion as a selective reducing agent
TL;DR: The use of modified boron hydrides as selective reducing agents for organic functional g roups has been studied extensively as discussed by the authors, with a focus on reducing a wide variety of organic functional groups with remarkable selectivity.
1.8K
Radioactive Labeling of Proteins in Vitro
Robert H. Rice,Gary E. Means +1 more
TL;DR: A procedure is described for the radioactive labeling of proteins in vitro in which 3H- or 14C-methyl groups are attached to the protein amino groups by reductive alkylation.
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