Posted Content10.17504/protocols.io.bp2l61mqrvqe/v1
10x Multiome Sample Processing v1
dr kemi22
- 26 May 2022
1
TL;DR: In this paper , a protocol is used to profile all the open chromatin regions at a single nuclei level through the rapid generation of NGS-ready libraries, and for rapid generation 3' transcriptomic-NGS-ready-single-cell-libraries from the same pool of transposed nuclei.
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Abstract: Protocol is used to profile all the open chromatin regions at a single nuclei level through the rapid generation of NGS-ready libraries, and for rapid generation of 3' transcriptomic-NGS-ready-single-cell-libraries from the same pool of transposed nuclei.
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A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain
Zizhen Yao,Cindy T. J. van Velthoven,Michael Kunst,Meng Zhang,Delissa A. McMillen,Changkyu Lee,Won Jung,Jeff Goldy,Aliya Abdelhak,Matthew Aitken,Katherine Baker,Pamela Baker,Eliza Barkan,Darren Bertagnolli,Ashwin Bhandiwad,Cameron Bielstein,Prajal Bishwakarma,Jazmin Campos,Daniel Carey,Tamara Casper,Anish Chakka,Rushil Chakrabarty,Sakshi Chavan,Min Chen,Michael Clark,Jennie Close,Kirsten Crichton,Scott Daniel,Peter DiValentin,Tim A. Dolbeare,Lauren Ellingwood,Elysha Fiabane,Tim P. Fliss,James Gee,James Gerstenberger,Alexandra Glandon,Jessica Gloe,Joshua Gould,James Gray,Nathaniel Guilford,Junitta Guzman,Daniel Hirschstein,Windy Ho,Marcus Hooper,Mike Huang,Madison T Hupp,Kelly Jin,Matthew R Kroll,Kanan Lathia,Arielle Leon,Su Li,Brian Long,Zach Madigan,Jessica Malloy,Jocelin Malone,Zoe Maltzer,Naomi Martin,Rachel A. McCue,Ryan McGinty,Nicholas Mei,J. Melchor,Emma Meyerdierks,Tyler Mollenkopf,Skyler Moonsman,Thuc Nghi Nguyen,Sven Otto,Trangthanh Pham,Christine Rimorin,Augustin Ruiz,Raymond Sanchez,Lane Sawyer,Nadiya V. Shapovalova,Noah Shepard,Clifford R. Slaughterbeck,Josef Sulc,Michael Tieu,Amy Torkelson,Herman Tung,Nasmil J. Valera Cuevas,Shane Vance,Katherine Wadhwani,Katelyn R. Ward,Boaz P. Levi,Colin Farrell,Rob Young,Brian Staats,Ming‐Qiang (Michael) Wang,Carol L. Thompson,Shoaib Mufti,Chelsea M. Pagan,Lauren Kruse,Nick Dee,Susan M. Sunkin,Luke A. Esposito,Michael Hawrylycz,Jack Waters,Lydia Ng,Kimberly A. Smith,Bosiljka Tasic,Xiaowei Zhuang,Hongkui Zeng +100 more
TL;DR: A comprehensive and high-resolution transcriptomic and spatial cell-type atlas for the whole adult mouse brain establishes a benchmark reference atlas and a foundational resource for integrative investigations of cellular and circuit function, development and evolution of the mammalian brain.
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