TL;DR: The efficiency compensation control facilitates identification of patient samples that are so contaminated with PCR inhibitory compounds that different amplification reactions are affected to a different extent and accurate quantitation of residual disease in these samples is therefore impossible with the current quantitative real-time PCR protocols.

TL;DR: Recent studies suggest a role for EBV viral load testing in nasopharyngeal carcinoma, Hodgkin's disease, and AIDS patients with brain lymphoma and further research is needed to define more fully the clinical utility of viral load tests in the full spectrum of EBV-associated diseases.

TL;DR: A novel platform for the electronic detection of nucleic acids on microarrays is introduced and shown to perform well as a selective detection system for applications in molecular diagnostics and pharmacogenetics.

TL;DR: In this paper, microsatellite analysis at chromosomal regions frequently altered in head and neck squamous carcinoma was performed on matched saliva and tumor samples from 37 patients who had OCC.

TL;DR: It is concluded that DNA recovery from LCM-sampled tissue is independent of the histological stain chosen to highlight nuclear detail, which is equivalent to the recovery after methyl green staining.

TL;DR: Comparative genomic hybridization (CGH) performed with DOP-PCR products from microdissected paraffin blocks allows for the accurate investigation of the cytogenetic characteristics from invasive tumors and of cytogenetics heterogeneity within neoplastic tissue.

TL;DR: In this paper, the authors describe optimized protocols for membrane and section preparation and for low temperature antigen retrieval that allow IHC and ISH to be used reliably on membrane mounted paraffin tissue sections.

TL;DR: Genes with strong hybridization signals and at least twofold difference were likely to be validated by real-time RT-PCR, and both hybridization intensity and the level of differential expression determine the likelihood of validating high-density filter array results.

TL;DR: Expression profiling refers to the process of establishing the pattern of expression of thousands of individual genes simultaneously in a given cell or tissue sample by extracting its RNA, converting it to cDNA and hybridizing the labeled cDNA to a DNA microarray as discussed by the authors.

TL;DR: In this paper, a patient was diagnosed with Pagetoid osteosarcoma and had a first-degree relative with history of the same, and the patient's tumor samples might contain a similar genetic abnormality.

TL;DR: FISH for chromosomes 1 and 8, extended by probes for chromosomes 6, 7 and X, represents a promising approach toward a more accurate differentiation between hepatocellular adenoma and carcinoma.

TL;DR: Cytogenetic or molecular cytogenetic analysis of four adamantinomas and a review of eleven cases in the literature reveals extra copies of chromosomes 7, 8, 12, 19, and 21 as recurrent in this neoplasm.

TL;DR: In this paper, the authors evaluate current diagnostic methods used for the evaluation of T cell receptor (TCR) gene rearrangements, 24 different laboratories analyzed 29 lymphoid neoplasm samples of extracted DNA and paraffin-embedded tissue and were asked to complete a technical questionnaire related to the testing.

TL;DR: Finkelstein et al. as discussed by the authors proposed a method for the detection of pathologies in the human brain using a protein-protein interaction (P-means-protein (P2P) model.

TL;DR: Fluorescence in situ hybridization using dual red and green DNA probes to regions of the X and Y chromosomes were used in one case where the potential contaminant was suspected to have originated from a male patient, and DNA polymorphisms were used to compare allelic patterns that were informative not only in proving the extraneous tissue as a contaminant, but also could be used to trace the latter to its original tissue source.

TL;DR: A generally useful, partially automated, human mutation detection method based upon printing moderate density oligonucleotide arrays using a biorobot on activated nylon membranes using the Beckman Biomek 2000 is reported on.

TL;DR: The assay provides a reliable quantification of the number of hK2 and PSA mRNA copies, allows to discriminate PC cases from healthy subjects, and offers a tool for further studies on molecular staging of PC.

TL;DR: It is concluded that the DHPLC is a fast, sensitive, cost-efficient, and reliable method for the scanning of INK4A somatic or germline mutations and polymorphisms of large number of samples.

TL;DR: Although biochemical testing remains the mainstay of diagnosis, molecular methods have acquired an essential role for identification of mutations and for assessment of at-risk pregnancies.

TL;DR: It is suggested that external controls for SMN gene dosage analysis should be chosen carefully with regard to SMN2 copy number, and the effect of heteroduplex formation should be considered when performing quantitative multiplex PCR.

TL;DR: Existing mechanisms for laboratory accreditation and oversight are best suited to be the nidus for any new oversight programs, including the Association for Molecular Pathology (AMP).

TL;DR: A novel efficiency compensation control (ECC) for the quantitation of cells with t(14;18) by real-time polymerase chain reaction (PCR) showed that a decrease in amplification efficiency in a sample caused an increase in the difference in threshold cycles for a multicopy 36-actin gene and a single copy albumin gene, both of which were co-amplified in the ECC reactions.

TL;DR: Kaul et al. as discussed by the authors introduced a new feature in the Journal of Molecular Diagnostics, "consultations in molecular diagnostics", which includes short case-directed articles meant to illustrate a classic principle, a difficult interpretation, or a new approach or application.

TL;DR: This assay will be used to test the hypothesis that sensitive and quantitative measurement of leukemic burden, during or after therapy of APL, can stratify patients into discrete risk groups, and thereby serve as a basis for risk-adapted therapy in APL.

TL;DR: The results further confirm that although KSHV is universally present in Kaposi's sarcoma and primary effusion lymphoma, it is not ubiquitious and supports the conclusion that K SHV is not associated with multiple myeloma.

TL;DR: The human leukocyte antigens (HLA) encoded by genes within the major histocompatibility complex display an impressive degree of polymorphism, apparently maintained in human populations through the need to successfully display a wide range of processed foreign peptides to the T cell antigen receptor.