TL;DR: Introducing into the wide host range conjugative plasmid RP4, a mini-Mu derivative which was known to be able to transpose spontaneously in E. coli K-12, and to induce in such a host several kinds of chromosomal rearrangements including replicon fusions, is introduced.
TL;DR: Results suggest the occurrence of natural plasmid transfer between staphylococci and soil bacilli, and rather unexpectedly, pBC16 is highly homologous to and incompatible with pUB110, an S. aureus plasmids specifying kanamycin resistance.
TL;DR: The data presented here permit the mapping of function onto the restriction enzyme maps of all three plasmids, showing a high degree of sequence conservation in the regions encoding replication and transfer functions but large differences in the areas which code for antibiotic resistance.
TL;DR: Results suggest that the protein is associated with the 5′ termini of pSLA2, which was senstive to a 3′-exonuclease, ex onuclease III, but was resistant to the 5″-ex onclease, λ-exOnuclease.
TL;DR: Physical mapping studies have allowed us to place the ac-u-c locus itself in a region of unique sequence DNA in a fragment, Ba10, which also includes the right-hand end of one inverted repeat.
TL;DR: It is demonstrated that pIP501 is transferred to Listeria at a mean frequency of 10−6.5 Md, and it is suggested that a circulation of plasmids can take place among members of the genera Listersia and Streptococcus.
TL;DR: The transposons Tn21, Tn501, and Tn1721 are related to Tn3 and transposition of a tnpA mutant of Tn 21 was restored in trans only by Tn20 itself, while elements of these elements did not complementtransposition of TN3.
TL;DR: The authors proposed to make a distinction between virulence (Vir) functions and oncogenic (Onc) functions of the octopine Ti plasmid of Agrobacterium tumefaciens, to indicate that certain functions essential for tumor formation outside the T-DNA region are probably expressed in the bacterium.
TL;DR: Octopine Ti plasmid derivatives that are ein− failed to establish—after introduction by transformation—in recipients carrying an octopineTi plasmids, and these derivatives were themselves susceptible to establishment inhibition.
TL;DR: The plasmid responsible for agrocin 84 biosynthesis by Agrobacterium radiobacter strain K84 has been genetically isolated free from any opine-catabolic plasmids as mentioned in this paper.
TL;DR: It is reported that respiratory-deficient strains of yeast can maintain plasmids containing Xenopus mitochondrial DNA with essentially the same facility as respiratory competent strains and data indicate that a recombinant DNA plasmid need not contain a yeast origin of replication in order for its replication to be under cell cycle control.
TL;DR: Results described here and elsewhere suggest that the instability of the deletion mutant reflects the loss of partitioning gene, a gene that is probably identical to an inc locus, inc D, that had been identified in this region in prior work.
TL;DR: The small circular DNA complexes have been found in every eucaryotic cell, primary lymphoid tissue cells of bursa and thymus, primary cell Lines of retina and liver, and established cultured cell lines of embryonal teratocarcinoma.
TL;DR: R plasmids of a new incompatibility group, IncHII, determined the constitutive production of H pili, had high molecular weights, and determined tellurite resistance, and are proposed to be called IncHI, the subgroups being HI1, HI2, and HI3.
TL;DR: Both A. rhizogenes plasmids share strong sequence homology with regions of the Ti plasmid that affect oncogenicity of A. tumefaciens C58, and possible analogies between hairy root and crown gall induction are discussed on the basis of the results presented.
TL;DR: Phage P1Km carries within the invertible DNA segment a 5-kb insertion with 0.8-kb terminal direct repeats flanking the kanamycin resistance determinant, which is thought to result from recombination between the flanking direct repeats.
TL;DR: Kinetoplast DNA from the insect trypanosome CrithidiaLuciliae contains a maxicircle of 22 × 106 D. fasciculata and restriction enzyme analysis indicates a sequence homology of more than 96% between the maxicircles of C. luciliae and C. fascICulata, which is not lower than that found between maxicIRcles of individual Trypanosoma brucei stocks.
TL;DR: Restriction endonuclease maps of two double-stranded plasmid-like DNAs isolated as linear molecules from the mitochondria of S-type cytoplasmic male-sterile maize were prepared and homologous sequence in addition to the 200-bp terminal inverted repeats was terminally oriented on both DNAs by reciprocal hybridization and heteroduplex analysis.
TL;DR: It appears that both plasmid replication and conjugation genes are responsible for host range in addition to the involvement of host gene products.
TL;DR: It is concluded that no fragment larger than 550 bp is perfectly conserved between all minicircles of the two trypanosomes and suggests that sequence rearrangements, rather than point mutations are the basis of the minicircle sequence heterogeneity.
TL;DR: The results are in favor of a plasmid epidemic and indicate a great stability of the transfer factor part of these plasmids under natural conditions.
TL;DR: Nine R plasmids originally harbored by Streptococcus faecalis were transferred by conjugation into various recipients in order to study the extent of their intraspecies, interspecies, and intergeneric host range.
TL;DR: Using both physical and genetical techniques, it is demonstrated that the [psi] determinant does not reside on other previously described yeast extrachromosomally inherited determinants; namely mitochondrial DNA, L double-stranded RNA, M double-StrandedRNA, and [2 μm] DNA.
TL;DR: It was found that multiplicity of infection in preparation of the transducing phage is the most important factor affecting the frequency of pBR322 transduction.
TL;DR: E. coli HB101 cells transformed to tetracycline resistance with the plasmids pMB9 or pBR322 display a 10 5 –10 6 -fold lower plating efficiency on agar containing 440 μ m CdCl 2 than nontransformed cells.
TL;DR: Physical characterization of the resident plasmids from Pseudomonas tabaci, P. angulata, and P. coronafaciens strains indicated that they harbored five different plasmid DNA species, two of which were named pJP1 and pJP2.
TL;DR: The transmission of the immunity by cytoduction was shown to be associated with the transfer of the light dsRNA segment and the immunity to the toxin encoded by the P1 virus of Ustilago maydis was mapped on the smallest of the viral ds RNA segments.
TL;DR: This work describes the incompatibility relationships and phenotypic properties of four probable members of Incompatibility group U including the proposed prototype, pAr-32, which has not been fully characterized.
TL;DR: Genes from the region spanning the sequences 44.1–49.3F appeared to affect the ability of mini-F plasmids to segregate into minicells, and this results argue for the sequestration of Mini F plasmid in host cells.
TL;DR: The method was applied for the labeling of a large cryptic plasmids present in Agrobacterium rhizogenes 1855 and for its subsequent ex planta transfer to a Ti plasmidless A. tumefaciens strain, which gave the new host the capacity to induce the hairy root disease in plants, and thus turned out to be an Ri plasid.