TL;DR: It is concluded that pSB2 in Y. pseudotuberculosis and Y. enterocolitica carry genetic information essential for virulence common to the Yersinia species, probably related to VW antigen production.

TL;DR: A method which allows isolation of deletions within hybrid plasmids is described, and it is found that Tc R mutants are usually maintained within the cell recombined with the parental Tc S plasmid.

TL;DR: The transposon Tn7 is used to isolate insertion and deletion mutations in the octopine Ti-plasmid pTiB6S3 and several regions involved in the determination of oncogenicity are positioned.

TL;DR: A simple and rapid method discriminating between covalently closed circular (CCC), open circular (OC), and linear (L) forms of plasmid DNA is presented.

TL;DR: Clinical isolates of Streptococcus faecalis were examined to determine whether such strains carried conjugative antibiotic resistance plasmids whose transfer was stimulated by sex pheromones, and a 35-Mdalton tetracycline-resistance plasmid called pCF-10 was identified.

TL;DR: It is proposed that the chromosome-mobilizing ability of R68.45 is due to the formation of an R.45-chromosome cointegrate during transposition of IS21, which would account for its high efficiency and the absence of a fixed chromosomal origin of transfer in Pseudomonas aeruginosa PAO, and its ability to function in a variety of bacterial hosts.

TL;DR: It is demonstrated here that the trfA region alone can provide the trans-acting functions necessary for replication from oriRK2, which is probably the primary event in RK2 replication and suggests that thetrfB region plays some other role in plasmid maintenance in plasmsids carrying all three regions.

TL;DR: The selectivity with which regions are transcribed in the tumor may indicate that the “T” DNA has “evolved” for best use in a eucaryotic cell.

TL;DR: It is reported that a fraction of the total cellular RNA from bloodstream form and culture form T. brucei hybridizes with the maxicircle; this hybridization is shown to be due to two abundant RNA species, the 9 S and 12 S RNAs.

TL;DR: Nine clinical isolates of group A, B, F, and G streptococci resistant to tetracycline, macrolides, lincosamides, and streptogramin B (MLS resistance) and to chloramphenicol were investigated for the conjugative transfer of the antibiotic-resistance markers into Streptococcal recipients.

TL;DR: Chloramphenicol resistance plasmid pCW7 and pC221 of Staphylococcus aureus have been characterized by the construction of detailed restriction maps and by the identification of restriction sites on both plasmids which map within either the structural gene encoding CAT or its controlling elements.

TL;DR: The pea ctDNA has been found to contain one set of ribosomal RNA genes by Southern hybridization of restriction endonuclease digest, R-loop studies, and DNA-DNA heteroduplex mapping.

TL;DR: Several wild-type virulent strains of Agrobacterium rhizogenes, belonging to both biotypes 1 and 2, were analyzed for plasmid content, finding that the plasmids of the various strains share rather extensive sequence homology.

TL;DR: The plasmid is potentially useful as a general purpose suicidal Tn carrier for bacteria that do not support stable replication/maintenance of ColE1 but are within the conjugational host range of RP4.

TL;DR: Data suggest a relationship between traB, Eex, and Phi(G101), and to facilitate future genetic comparison of the tra genes of R18 and other wide host range plasmids and the role of the host in conjugation, R18 DNA was compared with that of RP4, by restriction enzyme fragment patterns and found to be identical.

TL;DR: The plasmid pCS194, generated in vivo by recombination of two Staphylococcus aureus plasmids, pC194 and pS194, coding, respectively, for chloramphenicol and streptomycin resistance, can be replicated also in Bacillus subtilis in the presence of either of the two antibiotics.

TL;DR: In this paper, the 21 S rRNA gene and its precursors were localized in a detailed restriction enzyme map of the 12-kb mitochondrial genome of petite F11.

TL;DR: Fragments from the Bacillus bacteriophage φ105 have been cloned in recE+ and recE− bacteria lysogenic and nonlysogenic for the phage and the rate of spontaneous curing of the plasmid decreases in parallel to the appearance of deletions, presumably due to higher stability of the small plasmids.

TL;DR: In vitro recombination was used to generate RP4 plasmids with an inserted restriction fragment of bateriophage λ, suggesting the process of mobilization by RP4 may differ in certain features from chromosome transfer by F. coli K-12.

TL;DR: In this paper, a collection of 2000 Escherichia coli strains which harbor ColE1 plasmids carrying small random segments of the E. coli chromosome was screened for the correction of mutational defects in penicillin-binding proteins (PBPs): ponA (PBP-1a), ponB(PBP1b), dacB (pBP-4), and pfv (Pfv) were analyzed.

TL;DR: Examination of a series of cointegrates derived by recombination between various pairs of plasmids has revealed the existence of a new type of site-specific recombination that resembles that of prophage integration more than that of transposon insertion.

TL;DR: Restriction enzyme analysis of two Enhanced Chromosome Mobilizing plasmids derived from the IncP-1 plasmid R68 showed that they both possessed an additional segment of DNA, suggesting that pMO163 and pMO168 arise by the acquisition of a transposable element from the E. coli K-12 chromosome.

TL;DR: A small circular supercoiled DNA molecule species with a molecular weight of about 5.4 × 106 has been isolated from the extreme thermophile Thermus thermophilus HB8 and has a G plus C content of 68%, similar to that of the host chromosome.