Padjajaran Journal of Dentistry 

Abstract: Introduction: The primary aetiology of chronic periapical abscesses, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis, can be eliminated using intracanal medicaments such as calcium hydroxide. The purpose of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of calcium hydroxide paste in the vulnerable initial 7-day of treatment against E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277. Methods: An in-vitro laboratory experiment using a spectrophotometer as a microdilution method was conducted to determine bacteria's MIC and MBC values on calcium hydroxide paste. The sample used in this study was four bacteria using intracanal medicament of calcium hydroxide (Ca(OH)2), incubated for seven days at 37°C, and then bacterial growth was observed. The cell inhibition percentage was calculated using optical density measurements to determine the MIC value. The low MIC and MBC were defined as sensitive bacteria to calcium hydroxide. Results: Calcium hydroxide paste against E. faecalis (ATCC 29212) with MIC values at a concentration of 750 μg/ml and MBC values at a concentration of 96,000 μg ml; S. mutans (ATCC 25175) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 48,000 ug/ml; S. sanguinis (ATCC 10556) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 6,000 ug/ml; P. gingivalis (ATCC 33277) with MIC value at a concentration of 6,000 ug/ml and MBC value at a concentration of 48,000 ug ml. Conclusions: Calcium hydroxide can inhibit bacterial growth activity. E. faecalis (ATCC 29212) and S. sanguinis (ATCC 10556) are more sensitive to calcium hydroxide paste than other bacteria, with the lowest MIC and MBC on seven days of incubation since the maximum calcium and hydroxyl ions are released.

Abstract: ABSTRACTIntroduction: One of type 2 diabetes mellitus oral manifestations is xerostomia. Xerostomia can cause various problems, such as difficulty of eating, chewing and swallowing, so that it can affect the quality of life. The aim of this research is to analyze the effect of xerostomia on the quality of life of controlled and uncontrolled type 2 diabetes mellitus patients. Methods: This analytical observational cross-sectional study involving 92 subjects from type 2 diabetes mellitus patients aged 21 to 50 years (Patients of controlled and uncontrolled type 2 diabetes mellitus 46 subjects each). Xerostomia data was collected by Summated Xerostomia Inventory-Dutch Version questionnaire, while the quality of life data was collected by Xerostomia-related Quality of Life Scale (XeQoLs) questionnaire. All research data were analyzed descriptively and bivariate analysis with Chi-Square test. Results: The results showed that the quality of life of controlled and uncontrolled type 2 diabetes mellitus patients was equally low (50% in controlled type 2 DM patients and 64.9% in uncontrolled type 2 DM patients). This result showed that there is no difference between each subject groups. This also can be seen from the results of statistical tests that p = 0.193 (p>0.05), which means that there is no significant difference between the quality of life of controlled and uncontrolled type 2 diabetes mellitus patients. Conclusion: There was no effect between xerostomia of controlled and uncontrolled type 2 DM patients with their quality of life.Keywords: type 2 diabetes mellitus, xerostomia-related to quality of life, xerostomia

Abstract: ABSTRACT Introduction: Alginate is a type of elastic dental impression, which is an irreversible hydrocolloid. The main components in sodium alginate can be obtained naturally through the extraction process from seaweed (Kappaphycus alvarezii). Several physical characteristics must be fulfilled by dental impressions, such as dimensional stability related to imbibition and syneresis. ANSI/ADA specification No.18 regarding the maximum permitted change in alginate impression materials may not exceed 0.5%. This research aimed to analyze the value of imbibition and syneresis on dental impressions of red seaweed extract (K.alvarezii). Methods: The research was conducted using an experimental laboratory method with a post-test-only control group design. There were two groups, namely the control group of commercial dental impressions and the treatment group using dental impressions with the sodium alginate extracted from red seaweed (K.Alvarezii) by the acid method, and the FTIR test was performed to analyze compound sodium alginate. Imbibition and syneresis measurements were carried out using a calliper on a plaster cast model from negative mould casting results, where previously, the mould was soaked in water for imbibition and evaporated for syneresis. Results: Imbibition and syneresis tests showed that the impression material group with sodium alginate from red algae was significantly lower than those with commercial impression materials. It was concluded that the impression material with sodium alginate from red seaweed had an effect on reducing the percentage of syneresis (p≤0.05) and imbibition (p≤0.05) of the impression material. Based on the results of our research, it was found that the alginate impression material extracted from red seaweed (k.alvarezii) had an imbibition value of 0.33% (p≤0.05) and syneresis of 0.28%, (p≤0.05) lower compared to alginate impression materials. commercial alginate impression material. Conclusion: The imbibition and syneresis values of red seaweed alginate show an excessively low value compared to commercial alginates. KEYWORDS dental impression,sodium alginate, Kappaphycus alvarezii, imbibition, syneresis

Abstract: ABSTRACT Introduction: Process of dental plaque formation consists of three stages, pellicle formation, initial colonization facultative gram-positive one of microbes is S. mutans, secondary colonization and microbial maturation by Porphyromonas intermedia, P. gingivalis, Fusobacterium nucleatum. Formation of dental biofilm is considered to be main etiology of chronic periodontitis. Bacteria that often involved in chronic periodontitis are P. gingivalis. Tomatoes (Solanum lycopersicum L.) content lycopene compounds, flavonoids, and saponins have been shown to inhibit or kill bacterial growth. The aim is to analyze the antibacterial effect of servo tomato ethanol extract against S. mutans and P. gingivalis. Methods: This research was a laboratory experimental one with a posttest only control group design where observed variable diameter of the inhibition zone was produced from ethanolic extract of tomato (Solanum lycopersicum L.) in various concentrations of 3.125, 6.25, 12.5, 25, 50 and 100% chlorhexidine 0.2% as a positive control, negative control of distilled water against S. mutans and P. gingivalis on blood agar media. The method used in this study was the well-diffusion test. Results: The highest inhibitory zone at 100% concentration with a diameter of 32.10 mm was very strong; lowest inhibition at 3.123% was 3.95 mm, weak classification and against P. gingivalis at 3.125%, it was 3.72 mm, weak classification, meanwhile at 100% concentration, it was 9.67 mm, medium classification. The results of the One Way ANOVA statistical test showed a p<0.05 which had a significant effect in inhibiting the growth of S. mutans and P. gingivalis. Conclusions: There was an antibacterial effect of the ethanolic extract of tomato against S.mutans produced a larger diameter and stronger inhibition zone meanwhile against P.gingivalis produce medium inhibition zone. The content of tomatoes such as lycopene compounds, flavonoids, and saponins have been shown to inhibit or kill bacterial growth.Keyword : tomato ethanol extract (Solanum lycopersicum L.), S. mutans, P. gingivalis, antibacterial