Showing papers in "Journal of Microbiology in 2023"
TL;DR: This review describes the cellular phenomena that occur with temperature change and bacterial responses from a molecular perspective, mainly in Escherichia coli.
35 citations
TL;DR: In this article , the authors summarize the status of various CRISPR technologies that can be applied to synthetic biology and discuss the development of synthetic biology combined with CRISpl technology in microbiology.
Abstract: With developments in synthetic biology, “engineering biology” has emerged through standardization and platformization based on hierarchical, orthogonal, and modularized biological systems. Genome engineering is necessary to manufacture and design synthetic cells with desired functions by using bioparts obtained from sequence databases. Among various tools, the CRISPR-Cas system is modularly composed of guide RNA and Cas nuclease; therefore, it is convenient for editing the genome freely. Recently, various strategies have been developed to accurately edit the genome at a single nucleotide level. Furthermore, CRISPR-Cas technology has been extended to molecular diagnostics for nucleic acids and detection of pathogens, including disease-causing viruses. Moreover, CRISPR technology, which can precisely control the expression of specific genes in cells, is evolving to find the target of metabolic biotechnology. In this review, we summarize the status of various CRISPR technologies that can be applied to synthetic biology and discuss the development of synthetic biology combined with CRISPR technology in microbiology.
19 citations
18 citations
TL;DR: In this article , a review of the link between vaccine-induced immune responses and the gut microbiota using high-throughput technology and sophisticated system vaccinology approaches could provide crucial insights for designing effective personalized preventive and therapeutic vaccination strategies.
Abstract: Vaccines are one of the most powerful tools for preventing infectious diseases. To effectively fight pathogens, vaccines should induce potent and long-lasting immune responses that are specific to the pathogens. However, not all vaccines can induce effective immune responses, and the responses vary greatly among individuals and populations. Although several factors, such as age, host genetics, nutritional status, and region, affect the effectiveness of vaccines, increasing data have suggested that the gut microbiota is critically associated with vaccine-induced immune responses. In this review, I discuss how gut microbiota affects vaccine effectiveness based on the clinical and preclinical data, and summarize possible underlying mechanisms related to the adjuvant effects of microbiota. A better understanding of the link between vaccine-induced immune responses and the gut microbiota using high-throughput technology and sophisticated system vaccinology approaches could provide crucial insights for designing effective personalized preventive and therapeutic vaccination strategies.
12 citations
9 citations
TL;DR: Norovirus surveillance and genotyping studies still need to be further strengthened to increase knowledge regarding the pathogens and their variant characteristics, to better characterize the patterns of norovirus outbreaks and to provide information for outbreak prevention.
9 citations
TL;DR: Compared with AgNPs, PSP@AgNPs promote faster healing of infected wounds, and represent potential antibacterial agents against wound infections.
9 citations
TL;DR: Five transporters of manganese have been identified in Salmonella and it has been shown that yiiP expression is not dependent on MntS, but there might be additional transporters that need to be identified.
9 citations
TL;DR: Current research exploring the application of colicins and microcins, a subset of bacteriocins, with an emphasis on their activities against drug-resistant pathogens, both in in vitro and in vivo settings are highlighted.
8 citations
TL;DR: CXCL12/CXCR4 axis is involved in the recruitment of NK cells by HMGB1, contributing to persistent airway inflammation and AHR during the late stage of RSV infection.
6 citations
TL;DR: This review focuses on studies of the mechanisms of microbial interaction where the growth of other microbes is affected and some successful applications of microbial interactions in cultivation methods, an approach that can play an important role in the bioprospecting of untapped microbial resources.
Abstract: Abstract Pure cultivation of microbes is still limited by the challenges of microbial uncultivability, with most microbial strains unable to be cultivated under standard laboratory conditions. The experience accumulated from advanced techniques such as in situ cultivation has identified that microbial interactions exist in natural habitats but are absent in laboratory cultures. These microbial interactions are likely one of the key factors in isolating previously uncultured microbes. The need for better knowledge of the mechanisms operating in microbial interactions has led to various experiments that have utilized microbial interactions in different approaches to microbial cultivation. These new attempts to understand microbial interactions not only present a new perspective on microbial uncultivability but also provide an opportunity to access uncultured phylogenetically novel microbes with their potential biotechnology applications. In this review, we focus on studies of the mechanisms of microbial interaction where the growth of other microbes is affected. Additionally, we review some successful applications of microbial interactions in cultivation methods, an approach that can play an important role in the bioprospecting of untapped microbial resources.
TL;DR: Heterologous coexpression of a precursor peptide coding gene sinA and lanthipeptide synthetase coding gene sinKC in the host Escherichia coli strain BL21(DE3) resulted in the synthesis of a new lanthipeptide, sinosporapeptin, which contained unusual amino acids, including one labionin and two dehydrobutyrine residues, as determined using NMR and MS analyses.
TL;DR: Redundancy analysis (RDA) indicated that the rhizosphere microbial community of Fandi3 and Yunyan87 were highly correlated with various environmental factors and metabolites, which expand the understanding of the roles of tobacco cultivars in plant-micro-ecosystem interactions, and provide a basis for the control of tobacco bacterial wilt.
TL;DR: The findings suggest that NEDD4 may play a crucial role in regulating the GSDMD-mediated pyroptosis signaling pathway, and represents a promising approach to mitigate excess mortality during influenza pandemics by suppressing synergistic inflammation during co-infection of influenza A virus and Streptococcus pneumoniae.
TL;DR: Oral administration of L. rhamnosus KBL2290 modulated the levels of mRNAs encoding chemokines and markers of inflammation; increased regulatory T cell numbers; and restored tight junction activity in the mouse colon, indicating that oral L. rhamnosus KBL2290 may be a useful novel probiotic.
TL;DR: Recent findings relating to the host responses, in different types of cells in vitro and in animal models, mediated by Stxs-containing exosomes are summarized.
TL;DR: It was confirmed that astaxanthin biosynthesis related genes and cellular ROS levels were increased, and thus ultrasonic stimulation acts as an oxidative stimulus, and thus the novel approach based on the ultrasonic treatment would help to enhance the astaxanthin production from H. pluvialis.
TL;DR: An overview of PG assembly and antibiotics that target this pathway is provided and the implications of a common lipid carrier being used for assembly of PG and other surface glycopolymers in antibiotic development are discussed.
TL;DR: This review will focus on the processes necessary for the catabolic utilization of sialic acid in bacteria through the well-conserved catabolic pathway.
TL;DR: Two Gram-stain-positive, motile, endospore-forming, facultatively anaerobic strains, isolated from tidal flat sediment of the Incheon City on the west coast of Korea, represent two novel species in the novel genus Caldibacillus, for which the names Fervidibacillus albus gen. nov. and Fervidibacillus halotolerans sp.
TL;DR: Three novel bacterial strains, 321^T, 335^T, and 353^T, were isolated from the intestines of Aegosoma sinicum larvae collected from Paju-Si, South Korea and indicated that they were novel species belonging to the genus Luteibacter.
TL;DR: In this paper , two-month-old oil palm seedlings were artificially inoculated with Ganoderma boninense, and the isolates were categorized into three groups: highly aggressive (4A and 5B), moderately aggressive (5A and 7A), and less aggressive (2).
Abstract: Abstract Basal stem rot incidence caused by a white-rot fungus, Ganoderma boninense , is the major disease of oil palm in Southeast Asia. The rate of disease transmission and host damage are affected by variations in pathogen aggressiveness. Several other studies have used the disease severity index (DSI) to determine G. boninense aggressiveness levels while verifying disease using a culture-based method, which might not provide accurate results or be feasible in all cases. To differentiate G. boninense aggressiveness, we employed the DSI and vegetative growth measurement of infected oil palm seedlings. Disease confirmation was performed through scanning electron microscopy and molecular identification of fungal DNA from both infected tissue and fungi isolated from Ganoderma selective medium. Two-month-old oil palm seedlings were artificially inoculated with G. boninense isolates (2, 4A, 5A, 5B, and 7A) sampled from Miri (Lambir) and Mukah (Sungai Meris and Sungai Liuk), Sarawak. The isolates were categorized into three groups: highly aggressive (4A and 5B), moderately aggressive (5A and 7A), and less aggressive (2). Isolate 5B was identified as the most aggressive, and it was the only one to result in seedling mortality. Out of the five vegetative growth parameters measured, only the bole size between treatments was not affected. The integration of both conventional and molecular approaches in disease confirmation allows for precise detection.
TL;DR: The results showed that the glyceraldehyde triphosphate dehydrogenase promoter of Aspergillus oryzae with a unique structure displayed the most robust strength in constitutively controlling the expression compared to the PgpdA2 and other putative promoters tested.
TL;DR: Attention is suggested to the nitrogen and phosphorus requirements of slash pine plantations in the later stages of sandy management, as there was a significant increase in the relative abundance of functional groups involved in nitrogen fixation and uptake as stand age increased.
TL;DR: Comparative genomic analysis revealed that S. rugosoannulata is superior in utilizing aldehyde-containing lignins and is possible to utilize algae during the cultivation and is possible to utilize algae during the cultivation.
TL;DR: Fresh WMT moderated gut microbiome to improve the behavioral and sleeping disorder symptoms of ASD via decrease toxic metabolic production and improve detoxification, which provides a promising gut ecological strategy for ASD children and its related symptoms treatments.
TL;DR: Investigating the role of Tec proteins, which belong to the TEA/ATTS transcription factor family, in the hyphal development and virulence of M. lusitanicus suggests that Tec homologues promote mycelial development and virulence by enhancing mitochondrial metabolism and rhizoferrin accumulation.