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  4. 2012
Showing papers in "International Journal of Bioscience, Biochemistry and Bioinformatics in 2012"
Journal Article•10.7763/IJBBB.2012.V2.151•
Preparation of Biodiesel from Sunflower Oil by Transesterification

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Marimuthu Thirumarimurugan, V. M. Sivakumar, A. Merly Xavier, D. Prabhakaran, T. Kannadasan 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics

82 citations

Journal Article•10.7763/IJBBB.2012.V2.103•
Sensory Evaluation and Chemical Characteristics of Smoked Stingray (Dasyatis Blekeery) Processed by Using Two Different Liquid Smoke

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Fronthea Swastawati, Eko Harry Susanto, Bambang Cahyono, Wahyu Aji Trilaksono
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this article, the quality of smoked stingray (Dasyatis blekery) was determined by sensory and chemical analysis using corncob (CCLS) and coconut shells liquid smoke.
Abstract:  Abstract—The purpose of this research was to determine the quality of smoked stingray (Dasyatis blekery). The fish were divided into two groups; then processed using corncob (CCLS) and coconut shells (CSLS) liquid smoke. All of smoked stingray samples were subjected to sensory and chemical analysis. Sensory analysis on both samples was no statistically different. On the other hand, the lysine availability was different either in the in the raw materials or smoked samples due to smoking process and duration of storage. Different liquid smoke and storage time gave significant effect to lysine availabilty (p<0,05). Both liquid smoke gave significant effect (p<0,05) to PV (CSLS = 2,816 meq/kg & CCLS = 2,195meq/kg) and TBA (CSLS = 109,685 mg malonaldehide/kg & CCLS = 45,169 mg malonaldehide/kg), but during storage this value were decrease as an effect of antioxidant activities of phenolic compounds consist in each liquid smoke. In contrast, pH values were increase. Both liquid smoke were able to apllied as a method of smoking fish.

52 citations

Journal Article•10.7763/IJBBB.2012.V2.124•
Effects of D-Tagatose and Inulin on Some Physicochemical, Rheological and Sensory Properties of Dark Chocolate

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M. Shourideh, A Taslimi, Mh. Azizi, Ma. Mohammadifar
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: Inulin and D-tagatose are less sweet than the sucrose and vanillin (0.1%) were additionally sweetened with stevia as discussed by the authors, which was adjusted from the Concentration of Stevia.
Abstract: Alkalized cocoa powder (10-12%) (Shokinag cocoa, Germany), deodrised cocoa butter (KLK-Kepong, Malysia), Inulin GR (Orafti, Belgium), D-tagatose (Damhert,Belgium), Sucrose (Karoon Co., Iran), Soy Lecithin (Cargill, Netherland), Stevia (Stevian Biotechnology Co., Malysia) and vanilla powder (Panda, China) were used for the production of dark chocolates .Experimental samples were produced by mixing Sucrose, D-tagatose and Inulin powders(51.4%),cocoa powder(16%), cocoa butter (32%), lecithin(0.5%) and vanillin (0.1%). Since Inulin and D-tagatose are less sweet than the sucrose the samples were additionally sweetened with stevia. Concentration of stevia was adjusted from the

50 citations

Journal Article•10.7763/IJBBB.2012.V2.139•
X-Ray Peak Profile Analysis of Nanostructured Hydroxyapatite and Fluorapatite

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K. Venkateswarlu, D. Sreekanth, M. Sandhyarani, V. Muthupandi, Arumugam Chandra Bose, N. Rameshbabu 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this paper, X-ray peak profile analysis (XPPA) by modified Williamson-Hall (W-H) models was employed to estimate the microstructural parameters such as, crystallite size, lattice strain and lattice deformation stress and deformation energy density from the powder diffraction data obtained for the microwave synthesized hydroxyapatite (HA) and fluorapatitic (FA) nanoparticles prepared under identical processing conditions of mixing and aging.
Abstract: 389  Abstract—In present study, X-ray peak profile analysis (XPPA) by modified Williamson-Hall (W-H) models, namely W-H-isotropic strain model (W-H-ISM), W-H-anisotropic strain model (W-H-ASM) and W-H-energy density model (W-H-EDM), was employed to estimate the microstructural parameters such as, crystallite size, lattice strain, lattice deformation stress and deformation energy density from the powder diffraction data obtained for the microwave synthesized hydroxyapatite (HA) and fluorapatite (FA) nanoparticles prepared under identical processing conditions of mixing and aging. The as-prepared powder particles were also characterized by transmission electron microscopy (TEM) method. The average crystallite size values estimated for HA and FA by XPPA were correlated to their respective transmission electron microscopy (TEM) analysis results. In addition, the estimated values of HA and FA were correlated to their in-vitro dissolution characteristics studied by ethylenediamine tetra-acetic acid (EDTA) titrimetric method. It is found that the average crystallite size estimated by W-H models is in good agreement with TEM results. The controlled in-vitro dissolution behavior of FA was found to be resulted out of its higher crystallite size, lower lattice strain and lower dislocation density compared to that of HA.

40 citations

Journal Article•10.7763/IJBBB.2012.V2.108•
Chemical and Biological Treatment of Fish Canning Wastewaters

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Raquel O. Cristóvão, Cidália M.S. Botelho, Ramiro Martins, Rui A.R. Boaventura
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this article, the situation of different plants located north of Douro river, in Portugal, was analyzed, in order to propose various solutions to their problems, such as high water consumption and high organic matter, oil and grease and salt content in their wastewaters.
Abstract: — The main environmental problems of fish canning industries are high water consumption and high organic matter, oil and grease and salt content in their wastewaters. This work aims to analyze the situation of different plants located north of Douro river, in Portugal, in order to propose various solutions to their problems. Thus, initially it was made an identification and implementation of prevent and control pollution measures within the industrial units in order to reduce water consumption, minimize the wastewater production and reduce the pollutant load to treat. Then, the wastewater treatability was evaluated through a sedimentation and coagulation-flocculation process and through an aerobic biological degradation. In the sedimentation and coagulation-flocculation process two organic coagulants (RIPOL 070 and RIFLOC 1815), commonly used in wastewater treatment, were tested, leading to good results, especially in terms of oil and grease and total suspended solids removals. The best suspended solids removal efficiencies were 53% and 79%, using 400 mg/L of RIPOL 070 and 150 mg/L of RIFLOC 1815, respectively. At these dosages, both coagulants demonstrated excellent oil and grease removals, about 99% for RIFLOC 1815 and 88% for RIPOL 070. The aerobic biological treatment with activated sludge proved to be very adequate to organic matter removal. Two feed flow rates were tested and the highest TOC removal efficiency (96%) was verified with the lowest one (0.75 L/h), corresponding to a longer hydraulic retention time (8 h). So, the proposed sequential treatment, combining physico-chemical and biological processes, proved to be an effective alternative to start the fish canning wastewaters treatment for further reuse in the industrial process.

38 citations

Journal Article•10.7763/IJBBB.2012.V2.62•
Extraction of Human Genomic DNA from Dried Blood Spots and Hair Roots

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Nguyen Thi Hue, Nguyen Dieu Hoai Chan, Phan Tuan Phong, Nguyen Thuy Linh, Nguyen Dt Giang 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: An efficient phenol method with 2 steps of lyses which allows high yield of DNA concentration from dried blood and hair root and could be the useful tool which may apply for further molecular analysis studies using long storing time materials like dried blood spots and hairroot samples.
Abstract: In most studies about molecular genetics, molecular diagnostics, DNA extraction is considered as the very first step that will give a significant effective The material used for DNA extraction is vary from fresh blood to dried blood, tissue, oral mucosa and hair root… The dried blood and hair root are the two materials that easy to collect and can store for long term, which leads to more advantages for many time extractions to have fresh DNA and enough amount DNA for further studies This study introduces an efficient phenol method with 2 steps of lyses which allows high yield of DNA concentration from dried blood and hair root Comparing to the commercial QIAamp® DNA Mini Kit which applied for dried blood sample, DNA concentration extracted from this method was higher, the quality of DNA was equal and the cost is lower Comparing to salting out method, a cheap and safe method, which an applied for hair root, the two-step lysis phenolic method also give higher efficiency This method could be the useful tool which may apply for further molecular analysis studies using long storing time materials like dried blood spots and hair root samples The reliability and performance of the molecular diagnostic assays which are polymerase chain reaction (PCR), enzyme digestion and recombinant selection (1) are strongly influenced by the extraction of input material, in this case, the quantity and quality of nucleic acids from clinical samples Although blood is highly recommended for DNA extraction, for the experiments in the extremely hard working environment, blood may be difficult to be stored in long term Furthermore the ease of collecting, costs of transporting, costs of storing and pre-processing should be taken all into consideration how to get an optimal choice of samples Then despite of blood is the most commonly used sample since it provides enough and high quality DNA, people look for other sources which are low cost, easy to collect and convenient to store

30 citations

Journal Article•10.7763/IJBBB.2012.V2.92•
Dyeing of Cotton, Bombyx Mori and Eri Silk Fabrics with the Natural Dye Extracted from Tamarind Seed

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Supaluk Tepparin, Porntip Sae-be, Jantip Suesat, Sirisin Chumrum, Wanissara Hongmeng 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this article, the tamarind-seed dyes provided a reddish brown shade on the fabrics, which could be altered when the dyed fabrics were put through the washing process.
Abstract: This research studied the extraction of the natural dye from two different types of tamarind seed viz. sour-tamarind seed (TF) and sweet-tamarind seed (TO). The extracted dye solutions were prepared into a powder form and subsequently used for dyeing cotton and Bombyx Mori silk and Eri silk fabrics. From the experiment, the TF dye powder was easier soluble in water and could render a higher color strength than the TO dye powder. It was found for the tamarind-seed dyes that they could better exhausted on the silk fabrics than the cotton. Between the two silk fabrics, the dyes illustrated a higher degree of exhaustion on Bombyx Mori silk fabric than the Eri counterpart. The tamarind-seed dyes provide a reddish brown shade on the fabrics. This reddish brown shade could be altered when the dyed fabrics were put through the washing process. After washing, the shade of the dyed fabrics was more intense. The color fastness to washing of the dyed fabrics was in a good-excellent level. Even after experiencing 5-time washing, no significant shade change was observed on the dyed fabrics. The effect of mordants on the dyed fabrics was studied using three different mordants which were ZnSo4, K2Cr2O7 and FeSo4. The ZnSo4 mordant was found to enhance the color fastness to washing to the dyed fabrics without causing any shade change, while using K2Cr2O7 as a mordant led to a shade alteration into a yellow brown but helped to stabilize the dye molecule, so such shade obtained was not sensitive to the washing condition. For the FeSo4 mordant, it also caused the shade change on the dyed fabrics to a dull grey shade but providing an excellent color fastness to washing.

25 citations

Journal Article•10.7763/IJBBB.2012.V2.143•
Microalgae Photobioreactor for Nitrogen and Phosphorus Removal from Wastewater of Sewage Treatment Plant

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Rawiwan Boonchai, Gyu Tae Seo, Da Rang Park, Chung Yeol Seong
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: Results suggest that semi-continuous mode offers higher biomass production and appropriate HRT were needed for high N and P removals.
Abstract: showed growth rate of 0.452 d -1 and 0.277 d -1 in primary effluent and final effluent, respectively. 30% of N and 53% of P were removed from primary effluent and 44% of N and 84.2% of P were removed from final effluent. These results suggest that semi-continuous mode offers higher biomass production and appropriate HRT were needed for high N and P removals.

25 citations

Journal Article•10.7763/IJBBB.2012.V2.68•
The Impacts of Environment on Plant Products

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Ercan Ceyhan, Ali Kahraman, Mustafa Önder
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In case of environmental conditions change, plants will response as changing some of their metabolic activities, so that some of the quality parameters will change as mentioned in this paper, and some of them will be affected negatively.
Abstract: Plan t species depend on specific environment conditions to optimum growing. There are various agricultural areas that comprised from plant species which originated in climatically quite different regions. Plants absorb many elements from soil which have biological function and some of them are known as toxic also at low amounts. In case of environmental conditions change, plants will response as changing some of their metabolic activities. So that some of the quality parameters will change. Some of the plants can adopt as well as reasonable values, but some of them will be affected negatively. Although human are not able to or has limits to change ecological situations, they also able to recognize some characteristics of plants and again can develop new plant species and regulate or eliminate some of the harmful effects of environment on plant quality parameters for adaptation to different climatic conditions. If we know the effects of ecological conditions on plants, the definition and improvement of plant quality parameters will be easy. Therefore plants are the basic place of food chain; there are some sorrows about their quality and credibility.

21 citations

Journal Article•10.7763/IJBBB.2012.V2.120•
Development of a Medical Expert System as an Expert Knowledge Sharing Tool on Diagnosis and Treatment of Hypertension in Pregnancy

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J. Gudu, D. Gichoya, P. Nyongesa, Alex M. Muumbo
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: There is a need to develop the Medical Expert System (MES) as an expert knowledge sharing tool to be used by other medical personnel within the Reproductive Health Division who are not specialists in diagnosis and treatment of Hypertension in Pregnancy.
Abstract: Pregnancy has so far remained at the testing phase of its life cycle and is yet to be implemented. During the research, it was found that there is an acute shortage of specialist obstetricians in the Reproductive Health Division which implies that there is also scarce expert knowledge on the diagnosis and treatment of Hypertension in Pregnancy, yet the condition continues to kill many women of reproductive age in Kenya, hence the need to develop the Medical Expert System (MES) as an expert knowledge sharing tool to be used by other medical personnel within the Reproductive Health Division who are not specialists in diagnosis and treatment of Hypertension in Pregnancy.

19 citations

Journal Article•10.7763/IJBBB.2012.V2.69•
Malathion T oxicity: Effect on Some Metabolic Activities in Oreochromis Niloticus, the Tilapia Fish

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Gehan H. Fahmy
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: The data shows that the rate of oxygen consumption was declined during all the exposure periods and all biochemical parameters were found to be decreased in all tissues on comparison with control.
Abstract: Th e toxic effect of the insecticide malathion on oxygen consumption and some biochemical characteristics (total protein, carbohydrate and cholesterol in liver, muscle, kidney and gills) of the tilapia fish (oreochromis niloticus) were estimated. The data shows that the rate of oxygen consumption was declined during all the exposure periods. On the other hand, all biochemical's parameters were found to be decreased in all tissues on comparison with control. The results indicated the toxic nature of the insecticide malathion.
Journal Article•10.7763/IJBBB.2012.V2.118•
Genetic Diversity of Cultivated Barley Landraces in Iran Measured Using Microsatellites

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Hamed Khodayari, Hojjatolah Saeidi, Azadeh Akhavan Roofigar, Mohammad Reza Rahiminejad, Mohammad Pourkheirandish, Takao Komatsuda 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: It can be concluded that there is a high level of genetic diversity between the barely landraces in Iran and that the barely Iranian gene pool is valuable source to search for new useful alleles for crop improvement.
Abstract: The cultivated barley (Hordeum vulgare subsp. vulgare) is one of the major crops in the world, cultivated in all temperate areas. Because there is not enough information about genetic diversity of barley landraces in Iran, in this study the genetic diversity of its landraces was evaluated using 26 microsatellite markers. The genetic diversity between 42 accessions (13 accessions of two-rowed and 29 accessions of six-rowed barley) collected from various regions of Iran was assessed. A total number of 161 alleles were found from 26 SSR loci. A high level Results: The 26 primer pairs assayed 26 loci with 161 alleles (Table 2). About 31 of these alleles were rare (frequency 0.95). The overall of polymorphism information content (PIC; average = 0.663), genetic diversity (0.705) and allele number per locus (6.19) were observed. In dendrograms constructed based on the SSR data, the cultivars distichon (two-rowed) and hexastichon (six-rowed) were separated. Based on the results of this study, it can be concluded that there is a high level of genetic diversity between the barely landraces in Iran and that the barely Iranian gene pool is valuable source to search for new useful alleles for crop improvement. average number of alleles per loci was 6.19. The gene diversity between loci varied between 0.064 and 0.913. Total average genetic diversity of material was 0.705. Three of the 26 loci showed more than 10 alleles per locus. The alleles for the 26 loci were distributed among the four region of origin (W, SW, N and NE) as follows: 101 alleles for W, 90 for SW, 44 for N and 59 for NE. Only one allele each was detected for the Bmac067 Fig 1. Distribution of barley in different regions of Iran Introduction: Cultivated barley (Hordeum vulgare subsp. vulgare) is one of the most important crop cereal in the tribe Triticeae (poacea) that cultivated over the temperate regions. Based on several reports it has been originated from Fertile crescent in Near East or from Tibetan in the west China. Iran is placed in the southeastern edge of Fertile Crescent from where a, based on several evidences, the loci in the W accessions, the HVMLOH1A locus in the SW accessions, the EBmac602, HVMLOH1A, Bmac067 and EBmac906 loci in the N accessions and the WMC1A, HVMLOH1A and Bmac067 in NE accessions. In genetic distance based dendrogram, the accessions of two and six rowed barley were clearly separated. HH94SW
Journal Article•10.7763/IJBBB.2012.V2.140•
Cuffless Blood Pressure Estimation during Exercise Stress Test

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Soheil Mottaghi, Mohammad Hassan Moradi, L. Roohisefat
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: A new calibration method for blood pressure estimation during exercise stress test that increases the quality of estimation and increases the correlation coefficients between real and estimated blood pressures is proposed.
Abstract:  Abstract—Blood Pressure (BP) measurement without using a cuff is an interesting goal especially during Exercise Stress Test. Conventional methods like Cuff-based blood pressure measurement is not accurate enough due to high motion artifacts. High blood pressure during exercise test will cause pain and discomfort during inflation of cuff as well. Many researches have been conducted and shown Pulse Arrival Time (PAT), can be used in blood pressure estimation. This paper proposes a new calibration method for blood pressure estimation during exercise stress test that increases the quality of estimation. Besides PAT and heart rate, estimated blood pressure of previous stage is added as another input parameter. This technique increases the correlation coefficients between real and estimated blood pressures. We also show that by using ΔPAT, ΔHR instead of PAT and HR quality of estimation improves.
Journal Article•10.7763/IJBBB.2012.V2.78•
Theoretical Investigation Heat Transfer Mechanisms in Nanofluids and the Effects of Clustering on Thermal Conductivity

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Mohammad Hadi Pirahmadian, Azadeh Ebrahimi
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
Journal Article•10.7763/IJBBB.2012.V2.121•
Cost-Effective EEG Signal Acquisition and Recording System

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Sabbir Ibn Arman, Arif Ahmed, Anas Syed
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this paper, EEG signal acquisition principles are discussed, applied, and some suggestions for improving signal acquisition are presented.
Abstract: such a device are simplicity, ease of use and applicability. The uniqueness of this device is that it uses only three electrodes while most other systems use the 10-20 system of electrode placement which requires 21 electrodes. But the reduction in electrodes must not invalidate the output of the system, which is the EEG signal. For this reason, it is critical that the electrodes are placed in the scalp location where we can observe distinguishable change in the various wave patterns of the EEG signal. The main circuit is made of simple electronic elements. The circuit consists of an amplifier, filter and an opto-isolator. In this paper, EEG signal acquisition principles are discussed, applied, and some suggestions for improving signal acquisition are presented.
Journal Article•10.7763/IJBBB.2012.V2.91•
Dyeing and Spectroscopic Properties of Natural Dyes on Poly (Lactic Acid) and Poly (Ethylene Terephthalate) Fabrics

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Vorabodee Sriumaoum, Jantip Suesat, Potjanart Suwanruji
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this paper, the authors studied the dyeing properties and spectroscopic characteristics of natural dyes on PLA and PET fabrics and observed that the dyes exhibited a stronger fluorescence emission on PLA than PET for both turmeric and cassumunar dyes.
Abstract: The current research studied the dyeing properties and spectroscopic characteristics of natural dyes on PLA and PET fabrics. The natural dyes used were the dyes extracted from turmeric and cassumunar powder and the commercial dyes viz. Natural Orange and Natural Red Brown. The water solubility and partition of the dyes in octanol and water (logP) were studied. It was found that the dyes with poor water solubility that were the turmeric and cassumunar dyes exhibited a high degree of exhaustion and visual color yield (K/S) than the readily water soluble commercial dyes viz. Natural Orange and Natural Red Brown. Turmeric and cassumunar dyes can be used for dyeing PLA and PET fabrics to a medium-deep shade with the turmeric dye providing a deeper shade. The fluorescence properties of the turmeric and cassumunar dyes were examined on the PLA and PET fabrics and it was observed that the dyes exhibited a stronger fluorescence emission on PLA than PET for both turmeric and cassumunar dyes. Cassumunar dye displayed less fluorescence properties than turmeric dyes on both fabrics. The fluorescence emission properties of the turmeric dyes at different K/S levels were also investigated.
Journal Article•10.7763/IJBBB.2012.V2.82•
Statistical Validation of Heart Rate Measurement Using Modulated Magnetic Signature of Blood with Respect to Electrocardiogram

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Chee Teck Phua, Gaelle Lissorgues, Boon Chong Gooi, Bruno Mercier
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: Results obtained from the Bland and Altman Test affirms accuracy of MMSB to be used as an alternative to ECG system for HR measurements and supports the use of M MSB as a viable alternative toECG for measuring resting HR for healthy individuals.
Abstract:  Abstract—Heart rate (HR) is an important human physiological marker commonly used for the understanding of an individual's physical health. This paper adopts and outlines the magnetic method of non-invasive acquisition of blood pulse using the disturbance created by blood flowing through a localized magnetic field (i.e. Modulated Magnetic Signature of Blood - MMSB). The measurement setup is designed to acquire the MMSB and a gold standard instrumentation, Electrocardiogram (ECG) for 20 healthy subjects (10 males and 10 females, aged 18-22). Two independent measurements were conducted for each subject resulting in 40 HR measurements collected. Four statistical tests were applied to validate the instantaneous HR measured from MMSB waveform with respect to the ECG waveform. First, the Pearson Correlation and Coefficient of Determination was applied on the measured HR and both MMSB and ECG methods of measurements were positively correlated (R=0.99) with perfectly linear relationship (< 1% scores in the data due to error). Secondly, the Paired Student's t-test and the Wilcoxon Signed-Rank Test were applied on the measured HR and they showed no significant difference (at 95% limits of agreement) for the mean and median calculated from HR measured from the two methods. Lastly, the Bland and Altman Test is applied on the measured HR and the ±2*Standard Deviation (SD) is derived as 0.48 blood pulse per minute (bpm). This is not significant for measurement of resting HR for healthy individual, who has a typical resting HR of +1 bpm. The result obtained from the Bland and Altman Test affirms accuracy of MMSB to be used as an alternative to ECG system for HR measurements. Results obtained from all the four statistical tests are coherent and supports the use of MMSB as a viable alternative to ECG for measuring resting HR for healthy individuals.
Journal Article•10.7763/IJBBB.2012.V2.136•
Colour Removal of Direct Red Dye Effluent by Adsorption Process Using Rice Husk

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V. M. Sivakumar, Marimuthu Thirumarimurugan, A. M. Xavier, A. Sivalingam, T. Kannadasan 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this paper, a low-cost and effective adsorbent for the removal of Direct Red 23 from dye effluent and also to stud y the effect of concentration of dye solution and t he effect of amount of adaption on the percentage of removal of dye.
Abstract: To show that rice husk could be employed as low-cos t and effective adsorbent for the removal of Direct Red 23 from dye effluent and also to stud y the effect of concentration of dye solution and t he effect of amount of adsorbent on the percentage rem oval of dye. Azo dyes and their degradation product s such as aromatic amines are highly carcinogenic. Ad sorption of dyes is a new technology for treatment of waste water containing different types of dyes.A dsorption process is adopted for removal of direct d 23 dye from the dye effluent using rice husk as the adsorbent in treated and untreated form. The proce ss involves: washing and drying of rice husk at 105° c ,followed by soaking in 0.6 M Citric acid for 2 hou rs and heated to 120 °C.Further it is dried and washed repeatedly to obtain treated rice husk.This treate d and untreated rice husk are used for removal of Dir ect Red 23 dye. Dye solutions of different concentrations were prepared and a known amount of adsorbent were added to study the Effect of concentration of dye solution and effect of amount of adsorbent on the percentage of removal of Direct Red 23.
Journal Article•10.7763/IJBBB.2012.V2.66•
Salinity Induced Apoptosis in Root Meristematic Cells of Rice

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Mojtaba Yazdani, Majid Mahdieh
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: The results indicated that 500 mmol/L NaCl treatments could lead to specific features of apoptosis in root tips in both cultivars, such as DNA ladder, nuclear condense and deformation, and transferase mediated dUTP nick end labeling positive reaction, which were initiated at 4h of treatment and progressed thereafter.
Abstract: Salt stress is one of the most serious problems in agriculture and can induce apoptosis in plants. Cell death is induced by two modes: apoptosis and necrosis. We used a TUNEL assay and DNA gel electrophoresis on NaCl-treated cells of rice root to morphologically distinguish the mode of cell death in salt-sensitive and salt-tolerant rice cultivars. The results indicated that 500 mmol/L NaCl treatments could lead to specific features of apoptosis in root tips in both cultivars, such as DNA ladder, nuclear condense and deformation, and transferase mediated dUTP nick end labeling positive reaction, which were initiated at 4h of treatment and progressed thereafter.
Journal Article•10.7763/IJBBB.2012.V2.73•
Study on Carbon Distribution at Protein Regions of Disorder

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K. Akila, N. Sneha, E. Rajasekaran
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: The carbon distribution analysis program is able to identify the disordered region clearly and can further analysis the proteins for identification of mutational sites for stabilisation.
Abstract: Hydrophobic interaction is the dominant force in proteins that arises due to carbon. The content and distribution of this carbon make a protein to fold in some form to have a specific function. Diseased proteins are expectedly differing from this carbon distribution pattern. This is taken as focus of this work by taking neurodegenerative protein, the SOD for study. The carbon distribution analysis program is able to identify the disordered region clearly. A long stretch of hydrophilic/hydrophobic regions with different combination of amino acid is considered as disordered regions. This disorder is due to unfolding or misfolding. The program developd for this purpose can further analysis the proteins for identification of mutational sites for stabilisation.
Journal Article•10.7763/IJBBB.2012.V2.106•
Production of Enzyme and Growth of Aspergillus oryzae S. on Soybean Koji

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Chuenjit Chancharoonpong, Pao-Chuan Hsieh, Shyang-Chwen Sheu
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: Production of certain enzymes in soybean koji and potential to be applied for accelerating fish sauce fermentation are aimed at.
Abstract:  Abstract—Soybean koji is an important ingredient for traditional fermented food in South-East Asia and East Asia. It provides large amount of enzyme from koji mold, Aspergillus oryzae S., to digest nutrients in substrates. This study aimed at production of certain enzymes in soybean koji and potential to be applied for accelerating fish sauce fermentation. Koji containing 60% soybean was used as substrate to investigate the enzyme production by A. oryzae S. The growth of this mold was enumerated by potato dextrose agar. The mycelium development of A. oryzae S. was observed by scanning electron microscope. During koji production, pH of soybean koji increased from 6.32 to 6.07. It was caused by extracellular proteins production. The highest neutral protease, alkaline protease and amylase activities were 84.38, 41.35 and 200 unit/g of dry weight, respectively. Moreover, growing of enzyme activities on soybean koji correlated with the growth of this mold. Electron micrograph showed that spores of A. oryzae S. were formed after 48 h of cultivation period. Additionally, the highest enzymes activities were also shown in this stage.
Journal Article•10.7763/IJBBB.2012.V2.96•
Investigation of the Optimum Pretreatment Conditions for the Knitted Fabric Derived from PLA/Cotton Blend

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Issara Na Phatthalung, Porntip Sae-be, Jantip Suesat, Potjanart Suwanruji, Nantaporn Soonsinpai 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this paper, the authors compared the two-bath and one-bath scouring/bleaching processes for knitted fabrics of 100% cotton and PLA fabrics, and found that the one-bath process was the time-saving process.
Abstract:  Abstract— The pretreatment processes prior to dyeing which were the scouring and bleaching, for the knitted fabric of PLA/cotton blend were studied comparing with the 100% cotton and PLA fabrics. These two pretreatment processes were studied in a sequence (scouring followed by H2O2 bleaching) and altogether in the one-bath process. The scouring process of the blended fabric was compared when the different scouring agents were employed (NaOH, Na2CO3 and pectinase enzyme). The two-bath and one-bath scouring/bleaching processes had a signified effect on the strength of the fabrics because the pretreatment processes typically used for cotton are in the alkaline condition which can deteriorate the PLA fiber. Therefore, the pretreatment conditions for the PLA/cotton blended fabric needed to be optimized to avoid fiber damage. From the research, it was observed that the pretreatments for the blended fabric, which was to enter the pale-shade dyeing, were scoured using 10%owf pectinase (60C, 60 min) as it enhanced a satisfactory water absorbency to the fabric without causing any adverse effect on the fabric strength. For deep-shade dyeing, the blended fabric should undergo the one-bath scouring/bleaching with 7%owf H2O2 (100C, 60 min) which yielded a water absorbency and whiteness on the fabric to a standard level and the resulting fabric strength was highest and this one-bath pretreament was the time-saving process. high-CO2 emission and the wastes generated are also difficult to dispose of. Therefore, the researches have focused on an alternative environmental-friendly fiber which has comparable properties and can be used as a substitute of the conventional polyester fiber, poly (ethylene terephthalate) (PET). In 2002, Cargill Dow Polymer LLC, a joint venture between Cargill and Dow Chemical companies opened a production plant to produce a biodegradable polyester fiber, polylactic acid (PLA), with the purpose of replacing the petroleum-based PET fiber. The different structures between
Journal Article•10.7763/IJBBB.2012.V2.93•
Therapeutical Effect of Extracts of Terminalia chebula In Inhibiting Human Pathogens and Free Radicals

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Dolly Singh, Deepti Singh, Soon Mo Choi, Sun Mi Zo, Saet Byul Ki, Sung Soo Han 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: It is found that methanol extract as a potential bactericidal and potent antioxidant while aqueous extract showed the least potential as an antimicrobial agent, though a moderate antioxidant, may provide scientific rationale for the use of crude extract of the plant as a new drug compound as potential antioxidant and antimicrobialAgent.
Abstract: In recent years, multiple drug resistance has been developed due to indiscriminate use of existing antimicrobial drugs in the treatment of infectious diseases. Conventional antibiotics are strong medicines, which if not used in precise way may cause harmful effects. The major thrust is to establish alternative antimicrobial agent in order to treat microbial infections with less or no toxic effect to body and less negligible side effects. The herbal medicines have shown potential to overcome the limitation associated with conventional drugs. However, the appropriate choice of source for herbal medicine is also very important. Terminalia chebula possesses potential pharmaceutical activities and used in several Ayurvedic formulations. The study of solvent-free organic (ethylacetate, acetone, methanol of increasing polarity) and aqueous extracts of the fruits of T. chebula, showed the potential to reduce growth of microorganisms, minimizing the risk of infection, while optimizing the conditions to encourage healing. In our study we found, methanol extract as a potential bactericidal and potent antioxidant while aqueous extract showed the least potential as an antimicrobial agent, though a moderate antioxidant. The finding may provide scientific rationale for the use of crude extract of the plant as a new drug compound as potential antioxidant and antimicrobial agent. Terminalia chebula is used as a mild laxative and as an astringent against wounds and abscesses. Practictioners of folk medicine in India and Southeast Asia use the fruit for homeostatic, laxative and as cardiotonic. It is used as a remedy against a sore throat and cough, against diarrhoea connected with a prolapsed rectum in China and against ulcers and dysentery in Tibet. The antibacterial activity of various extracts of Chebula myrobalan powder was tested against Streptococcus species, Staphylococcus aureus and Pseudomonas aeruginosa. II. MATERIALS T. chebula powder was purchased from local market of Mumbai, India. Pathogens of MTCC grade were obtained from IMTECH (Chandigarh, India). Gallic acid and Glutaraldehyde was purchased from s.d. fine-chemicals limited (Mumbai, India). Gentamicin and 2,4,6-tripyidyl-s-triazine (TPTZ) were procured from Hi-Media(Mumbai,India), 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT, 98%) reagent, 4'-6-diamidino-2-phenylindole (DAPI), propidium iodide (PI) and nystatin was purchased from Sigma (St. Louis, USA). Fetal bovine serum (FBS) and streptomycin-penicillin antibiotic solutions were bought from HyClone (Utah, USA). All other chemicals used were of analytical grade. SIRC (rabbit corneal epithelial cells) cell line was obtained from NCL, Pune, India
Journal Article•10.7763/IJBBB.2012.V2.72•
Isolation of Saprolegnia and the Influence of RootEthanolic Extract of Ruta graveolens on Saprolegnia. SppGrowth

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Karouei S. M. Hashemi, Haji M. Sadeghpour, Azizi I. Gholampour
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
Journal Article•10.7763/IJBBB.2012.V2.109•
Extraction of Carboxylic Acids from Hydrocarbon Mixture Using Imidazolium Ionic Liquids

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Hasiah Kamarudin, M.I. Abdul Mutalib, Zakaria Man
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
Journal Article•10.7763/IJBBB.2012.V2.133•
Endothelialization of Acellular Porcine ECM with Chemical Modification

[...]

Yao Wang, Tomer Bronshtein, Udi Sarig, Freddy Yin, Chiang Boey, Subbu S. Venkatraman, Marcelle Machluf 
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: Preliminary results showed that future efforts combining co-culture, treated scaffolds and dynamic culture environment may result in re-endothelialization leading to functional blood vessels in thick engineered tissue for partial cardiac replacement therapy.
Abstract: Vascularization remains a critical requirement for the long term survival of engineered tissue constructs, especially thick ones. Such thick constructs for cardiac tissue engineering has been reported by our group and others based on decellularized porcine cardiac extracellular matrix (pcECM) that has been shown to resemble the native tissue both structurally and chemically. The network of inherent vasculature, which was largely retained within our pcECM, can be used as primers for re-endothelialization and neo-vascularization with regenerative cells. Endothelial cells alone, seeded onto the ECM, not only attached and survived but also rearranged into typical confluent monolayer with self-alignment. Sequential co-cultures of human umbilical vein endothelial cells (HUVEC) and mesenchymal stem cells (MSC) were shown to support the growth of both lineages on the surface and in the vasculature of reseeded pcECM. After ECM treatment with gelatin or fibronectin, cell proliferation increased significantly for both MSCs and HUVECs. Preliminary results showed that future efforts combining co-culture, treated scaffolds and dynamic culture environment may result in re-endothelialization leading to functional blood vessels in thick engineered tissue for partial cardiac replacement therapy.
Journal Article•10.7763/IJBBB.2012.V2.122•
Three-Dimensional Numerical Analysis of Pulsatile Blood Flow around Different Plaque Shapes in Human Carotid Artery

[...]

Absaar ul Jabbar, Rana Usman Ali, Khalid Parvez, Umar H. K. Niazi
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
Journal Article•10.7763/IJBBB.2012.V2.131•
Hepatoprotective Effect of Garlic Homogenate Co-Administered with Anti-Tuberculosis Drugs in RatLiver Enzymes

[...]

A. Nasiru, I. G. Hafsat, M. M. Mohammad, A. A. Sabo
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
Journal Article•10.7763/IJBBB.2012.V2.64•
Degradation of Endosulfan by Mixed Bacterial Cultures Enriched from Endosulfan Contaminated Soils of Southern India

[...]

Sarah Sunitha, V. Krishnamurthy, Riaz Mahmood
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: The microorganisms present in this soil were found to possess the ability to degrade endosulfan as they are chronically exposed to it, and can be used as bio remediating agents for soil and water.
Abstract: Endosulfan is a characteristic pesticide having both chlorine and sulphur as functional groups, effective in controlling a large spectrum of pests. It is sprayed as contact insecticide on food crops. Residues of α and β endosulfan have varying half life and persist in air, water and soil along with their degradation products. The present study aimed at delineating endosulfan residues in soil, representing samples drawn from agricultural fields during winter season. The samples after pre-treatment were subjected to enrichment of the residues. The residues were estimated by GC-ECD method. The concentration of α endosulfan ranged from 0.1 mg to 29 mg/Kg, the levels of β Endosulfan ranged from 0.1-167 mg/kg, and that of Endosulfan sulphate ranged from 0.12-27 mg/Kg respectively. This study once again proves the highly persistant nature of the residues of endosulfan in soil and hence the need to find measures to remove them from the environment. The microorganisms present in this soil were found to possess the ability to degrade endosulfan as they are chronically exposed to it. Up to 70% of endosulfan and 100% of Endosulfan sulphate degradation were observed. Hence, organisms isolated from endosulfan contaminated soils by the process of enrichment can be used as bio remediating agents for soil and water.
Journal Article•10.7763/IJBBB.2012.V2.76•
Fabrication and Characterization of Nanostructured Polymer Composites Prepared by Melt Compounding

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Abdolmajid Alipour
01 Jan 2012-International Journal of Bioscience, Biochemistry and Bioinformatics
TL;DR: In this article, the effect of different percentages of nanoclay and matrix compositions on the characteristics of NR/BR blend prepared by open two-roll mill was investigated, and the results showed that the nanocomposites structure consisting of intercalated structure as well as partially exfoliated structure are obtained.
Abstract: In this research we have investigated the effect of different percentages of nanoclay and matrix compositions on the characteristics of NR/BR blend prepared by open two-roll mill. The dispersion of the layered silicate in rubber matrix was characterized by X-ray diffraction (XRD) The results showed that the nanocomposites structure consisting of intercalated structure as well as partially exfoliated structure are obtained. Properties such as permeability and complex viscosity as well as storage modulus are tested to assess the effect of addition of the nanoclay and matrix composition. Permeability and compression set of the nanocomposite samples received markedly improvement. These results were confirmed by Gel content measurements since a noticeable decrease in the gel values was observed when the organoclay was added to the rubber compounds. The properties improvement caused by nanoclay are attributed to intercalation of the organophilic clay by NR/BR macromolecules and established interaction between nanoclay and polymer matrix which was evidenced by SEM photomicrographs. These results were accompanied by enhanced rheological properties.
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