Journal• ISSN: 1061-9526
Electro- and Magnetobiology
Marcel Dekker
About: Electro- and Magnetobiology is an academic journal. The journal publishes majorly in the area(s): Magnetic field & Chemistry. It has an ISSN identifier of 1061-9526. Over the lifetime, 237 publications have been published receiving 3062 citations.
Journal Tools
Papers
TL;DR: This study examines how electric fields interact with and polarize a system of cells and considers how cell density and organization impact on induced cell transmembrane potential due to an external electric field.
Abstract: Electrochemotherapy is a novel technique to enhance the delivery of chemotherapeutic drugs into tumor cells. In this procedure, electric pulses are delivered to cancerous cells, which induce membrane permeabilization, to facilitate the passage of cytotoxic drugs through the cell membrane. This study examines how electric fields interact with and polarize a system of cells. Specifically, we consider how cell density and organization impact on induced cell transmembrane potential due to an external electric field. First, in an infinite volume of spherical cells, we examined how cell packing density impacts on induced transmembrane potential. With high cell density, we found that maximum induced transmembrane potential is suppressed and that the transmembrane potential distribution is altered. Second, we considered how orientation of cell sheets and strands, relative to the applied field, affects induced transmembrane potential. Cells that are parallel to the field direction suppress induced transmembrane po...
119 citations
TL;DR: The effects of weak microwave fields on the amounts of heat-shock proteins in cell cultures at various temperatures can be considered to be athermal, since the field strength was much lower than the safety standard for absence of heat generation by microwave fields.
Abstract: This paper describes the effect of weak microwave fields on the amounts of heat-shock proteins in cell cultures at various temperatures. The field was generated by signal simulation of the Global System for Mobile communications (GSM) of 960 Mhz, used in portable phones. Transformed human epithelial amnion (AMA) cells, growing on glass coverslips, were exposed in a transverse electromagnetic (TEM) cell to a microwave field, generating a specific absorption rate (SAR) of 2.1 mW.kg 1 in the cells. Exposure temperatures were 35, 37, and 40 0.1°C, respectively, and the exposure time was 20 min. The heat-shock proteins Hsp-70 and Hsp-27 were detected by immunofluorescence. Higher amounts of Hsp-70 were present in the cells exposed at 35 and 37°C than in the sham-exposed cells. These effects can be considered to be athermal, since the field strength was much lower than the safety standard for absence of heat generation by microwave fields. There was no significant response in the case of Hsp-27.
102 citations
TL;DR: Experimental results presented in this paper support Pohl's idea of existence of the electromagnetic field around yeast cells.
Abstract: Electromagnetic activity around yeast mitotic cells (Saccharomyces cerevisiae) was measured in the frequency range 8–9 MHz and special care was taken to extract reliable information from the raw signals. The characteristic of cold-sensitive tubulin mutants tub2-401 and tub2-406, which come to arrest before mitosis at a restrictive temperature (14°C) and which re-enter mitosis upon a shift back to a permissive temperature (28°C), was used to prepare synchronized mitotic cells. Immunofluorescence microscopy using an antitubulin antibody was used to analyze microtubular structures. The arrested tub2-401 mutant that had back-shifted to permissive temperature displayed no microtubules and no electromagnetic activity around the cells. In contrast, the arrested cells of the mutant tub2-406 displayed developed, but aberrant, nonfunctional microtubules and a high electromagnetic activity around the cells. The electromagnetic activity around the arrested mutant tub2-401 back-shifted to permissive temperature peaks ...
81 citations
TL;DR: The results showed that electropermeabilization of almost all cell lines tested occurred at 600 V/cm, and a marked difference in electrosensitivity existed among these cell lines, which could be of great importance for pharmacological and biochemical studies in vilro.
Abstract: Electropermeabilization (electroporation) is a technique widely used to introduce various membrane-impermeable molecules into cells in vitro or in vivo. In this study we determined the effect of different electric-field intensities on electropermeabilization and electrosensitivity of a variety of tumor-cell lines in vitro. For this purpose we used two assays: propidium iodide uptake for measurement of cell electropenneabilization, and the clono-genic or MTT assay for determination of electrosensitivity. Our results showed that electropermeabilization of almost all cell lines tested occurred at 600 V/cm. In contrast, a marked difference in electrosensitivity existed among these cell lines. Our results could be of great importance for pharmacological and biochemical studies in vilro, and for prediction and determination of tumor response in vivo to electropermeabilization combined with chemo-therapeutic drugs (electrochemotherapy) and gene therapy.
74 citations
TL;DR: An increase in the basal levels of these normally expressed transcripts is observed with dependence on both field strength and time of exposure, and a “window” effect is found for each transcript at 20 min exposure.
Abstract: Short exposure of cultured human cells to extremely low-frequency electromagnetic fileds causes a measurable increase in some transcript levels. The effect of varying field strength and exposure time on c-myc, β-actin, c-src, β-tubulin, and histone H2B transcript levels in human HL-60 cells exposed to continuous sine waves (60 Hz) is analyzed here. An increase in the basal levels of these normally expressed transcripts is observed with dependence on both field strength and time of exposure. A “window” effect is found for each transcript at 20 min exposure [magnetic flux density of 5.7 µTesla (rms)]. Alpha-globin, a transcript not normally expressed in this cell line, was unaffected by exposure to the field strengths used for these experiments.
72 citations
Related Journals (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2001 | 29 |
| 2000 | 30 |
| 1999 | 24 |
| 1998 | 27 |
| 1997 | 26 |
| 1996 | 25 |