About: Cryoletters is an academic journal published by Cryo-Letters. The journal publishes majorly in the area(s): Medicine & Cryopreservation. It has an ISSN identifier of 0143-2044. Over the lifetime, 37 publications have been published receiving 499 citations.
TL;DR: Methode de cryoconservation par azote liquide d'embryons matures issus of 4 varietes de cocotiers (10 a 12 mois apres la pollinisation) : technique et effets du pretraitement, survie et germination des embryons
Abstract: Methode de cryoconservation par azote liquide d'embryons matures issus de 4 varietes de cocotiers (10 a 12 mois apres la pollinisation) : technique et effets du pretraitement, survie et germination des embryons
TL;DR: Apices of in vitro plantlets of Coffea racemosa and C. sessiliflora withstood freezing in liquid nitrogen using the encapsulation-dehydration technique and two growth recovery patterns were observed: direct development of foliar primordia and callusing.
Abstract: Apices of in vitro plantlets of Coffea racemosa and C. sessiliflora withstood freezing in liquid nitrogen using the encapsulation-dehydration technique. An intense intracellular starch accumulation was noted after overnight culture of apices on standard medium. Apices of C. sessiliflora required a 3 to 10-d pregrowth in liquid medium containing 0.75 M sucrose, whereas those of C. racemosa required a progressive increase of the sucrose concentration from 0.5 to 1 M. After the dehydration phase, cells of some apices were severely damaged, showing osmiophilic granules on the surface of the plasmalemma. In other apices, cells were less severely damaged, showing exocytosis vesicles at the periphery of the plasma membrane. After freezing, survival rates of 27 and 37% were obtained for C. racemosa and C. sessiliflora, respectively. Two growth recovery patterns were observed: direct development of foliar primordia and callusing. (Resume d'auteur)
TL;DR: Reponse d'embryons immatures de cocotiers a la congelation par azote liquide (-196¼C) : survie et germination.
Abstract: Reponse d'embryons immatures de cocotiers (7 a 8 mois apres la pollinisation) a la congelation par azote liquide (-196¼C) : survie et germination