Abstract: Objective To investigate the clinical efficacy of apapatinib-targeted therapy combined with oxaliplatin and tiggio for treatment of gastric cancer. Methods A total of 150 patients with advanced gastric cancer were enrolled from March 2015 to March 2017. According to the random number table method, the patients were divided into the study group (apocitinib combined with oxaliplatin + tiggio) and the control group (oxaliplatin + tiggio), 75 cases each. The recent treatment effects and adverse reactions were compared between the two groups. Results The objective response rate (46.7% vs. 25.3%) and disease control rate (76.0% vs. 48.0%) in the study group and the control group were statistically significant (all P 0.05). The incidence of complications such as hypertension, proteinuria and hand-foot syndrome in the study group was higher than those in the control group (all P < 0.05), but it was in a state of grade Ⅰ~Ⅱ and tolerance. Conclusion Apotiphene combined with oxaliplatin and tiggio as a chemotherapy regimen for advanced gastric cancer may have a better effect. Key words: Gastric neoplasms; Drug therapy, combination; Apotinib; Oxaliplatin; Tiggio

Abstract: Objective To investigate the clinical effects and advantages of flexible 3D laparoscopic surgery on rectal cancer after neoadjuvant chemoradiotherapy (nCRT). Methods The data of 152 patients who received laparoscopic rectal cancer resection after nCRT excluding the cases of high rectal cancer, cardiac and pulmonary dysfunction were analyzed from January 2015 to January 2016 in the Department of Colorectal Surgery of Liaoning Cancer Hospital. The distances from the annal edge to the inferior tumor margin were within 8 cm in these patients. Among these patients, 76 cases received the 3D laparoscopic surgery after nCRT (3D-nCRT), and 76 cases undergone the 2D laparoscopic surgery after nCRT (2D-nCRT). Results Between two groups, the number of lymph node harvest (14.8±2.1 vs. 14.3±1.7), positive rate of the distal margin [1.3% (1/76) vs. 2.6% (2/76)], reserving anus rate [92.1% (70/76) vs. 81.2% (67/76)], local recurrence rate [1.3% (1/76) vs. 3.9% (3/76)] and anastomotic leakage rate [2.6% (2/76) vs. 3.9% (3/76)] had no statistical differences (all P > 0.05), while the operative time [(125.3±10.2) min vs. (136.6±12.0) min], intraoperative bleeding [(54.1±23.2) ml vs. (61.9±19.5) ml], anus exhaust time [(43.5±5.0) h vs. (45.4±5.6) h] and positive rate of circumferential resection margin (CRM) [1.3% (1/76) vs. 9.2% (7/76)] had statistical differences (all P < 0.05). Conclusion Flexible 3D laparoscopic surgery on rectal cancer after nCRT can shorten operative time, reduce intraoperative bleeding, alleviate the influence of intestinal peristalsis function,and improve operative quality. Key words: 3D Laparoscopy; Rectal neoplasms; Laparoscopic surgery; Neoadjuvant chemoradiotherapy; Surgical procedures, minimally invasive

Abstract: Objective To investigate the clinicopathologic features, diagnosis and differential diagnosis of granulocytic sarcoma (GS) of the vulva. Methods One patient with GS of the vulva was analyzed on morphological characteristics, immunohistochemical stainings and clinical data with review of the literature. Results The patient was a 75-year-old lady. She was admitted to the hospital for a painless lump found in her left vulvar region. B-ultrasound revealed a solitary isoechoic mass at she left labia minora with an internal heterogeneous echo. Grossly, the tumor was described as a solid neoplasm with fish-fresh like cut surface and an ill-defined margin. Microscopically, the malignant cells grew in a diffuse sheet pattern and were small to medium in size, with scant cytoplasm. The nuclei were round or oval, and had finely dispersed chromatin. The mitotic rates were high. Immunohistochemically, the tumour cells expressed for myeloperoxidase (MPO), CD43, lysozyme and CD117. Conclusions GS of the vulva is extremely rare. In routine histopathology, it may be misdiagnosed as lymphoma, Ewing sarcoma and small cell carcinoma et al. Awareness of the morphology and precise diagnosis of GS of the vulva have significance for appropriate treatment and prognosis of the patients. Key words: Vulvar neoplasms; Sarcoma, maeloid; Pathology, clinical; Diagnosis, differential

Abstract: The inhibiting mechanisms of brucine to cancer cells included inducing the apoptosis of cancer cells, inhibiting the proliferation of cancer cells, preventing cell adhesion and transfer. This paper reviews the function of brucine and its anti-tumor preparations, and compares the efficacy and adverse reactions between brucine related preparations and traditional drugs to evaluate its anti-tumor effects. Furthermore, more exploration on the inhibiting mechanisms is necessary for the patients to obtain safe and effective drugs. Key words: Anti-neoplastic angets, phytogenic; Brucine; Preparations; Drug therapy

Abstract: Objective To explore a desirable therapeutic regimen, which is effective, reasonable and practicable for locally advanced nasopharyngeal cancer (LA-NPC) patients in the era of IMRT, with a potential of translating into survival improvement of these patients. Methods Patients presented with stage Ⅲ-ⅣB, WHO type Ⅱ or Ⅲ NPC were randomly assigned to receive concurrent chemoradiotherapy (CCRT group) (45 cases) or induction chemotherapy plus radiotherapy alone (IC+ RT group) (43 cases), with random number table. IMRT and rapid arc planning were generated in the same treatment planning system for all patients. These two arms received docetaxel plus cisplatin for first cycle of chemotherapy, and cisplatin only for the second cycle. Results Forty-five patients received CCRT and 43 received IC+ RT. All the patients completed two cycles chemotherapy. Compared to the CCRT group, the incidence rate of grade 2-4 leukopenia was lower in IC + RT group[67.4% (29/43) vs. 86.7% (39/45), χ2= 4.628, P= 0.031], while the incidence rate of dermatitis, mucositis, neutropenia and fungal infection of oral cavity had no significant differences (all P > 0.05). The tumor response rate (95.3% vs. 100.0%, P= 0.236), 2-year overall survival rate (95.5% vs. 94.2%, P= 0.627), 2-year progression free survival rate (94.6% vs. 88.6%, P= 0.303), 2-year local recurrent free survival rate (97.3% vs. 95.5%, P= 0.951), 2-year regional recurrent free survival rate (94.7% vs. 96.2%, P= 0.949), and 2-years distant metastasis free survival rate (93.7% vs. 91.5%, P= 0.454) of the two groups were similar while comparing CCRT to IC+ RT group. Conclusion Combined IMRT with TP/DDP regimen, the efficacy of IC+ RT is similar to CCRT for LA-NPC. The IC+ RT group has less severe leukopenia than CCRT group, which is worth further study. Key words: Nasopharyngeal neoplasms; Radiotherapy planning, computer-assisted; Antineoplastic combined chemotherapy protocols

Abstract: Objective To observe the expressions of Slug, BRAF V600E and STIP1 proteins in papillary thyroid carcinoma (PTC), and to explore their correlation with capsular invasion and regional lymph node metastasis. Methods Slug, BRAF V600E and STIP1 expressions in 107 cases of differentiated PTC were examined by immunohistochemical staining. The expressions of three proteins and clinicopathological data were statistically analyzed. Results Positive rates of Slug, BRAF V600E and STIP1 in PTC were 65.4% (70/107), 61.7% (66/107) and 66.4% (71/107), respectively, and overexpression of Slug, BRAF V600E and STIP1 was significantly associated with capsular invasion and regional lymph node metastasis in PTC (P < 0.05). There are a significant correlation between expression of Slug and BRAF V600E in PTC (r= 0.235, P < 0.05). Conclusion Overexpression of Slug, BRAF V600E and STIP1 proteins is associated with capsular invasion and regional lymph node metastasis in PTC, which maybe useful for predicting regional lymph node metastasis and prognostic evaluation. Key words: Thyroid neoplasms; Carcinoma, papillary; Slug; BRAF V600E; STIP1; Lymph nodes metastasis; Prognosis

Abstract: Objective To study the role of fibulin-3 in blood and pleural effusion in the early diagnosis of malignant pleural mesothelioma (MPM). Methods Chinese and English literatures about fibulin-3 in the diagnosis of MPM before February 1, 2017 were searched through the computer. After the screening according to the criteria, literature data were extracted to build the analysis model and explore the origin of the heterogeneity. Results According to the selection criteria, 9 literatures including 26 independent study were taken into Meta analysis eventually. The level of fibulin-3 in blood and pleural effusion in the experimental group was higher than that in the control group by using the random effect model. The region subgroup analysis showed that the fibulin-3 level in the experimental group was higher than that in the control group among European and American people. Conclusion Fibulin-3 in the diagnosis of MPM has a higher sensitivity and heterogeneity, which could be served as an auxiliary method in early diagnosis of MPM. Key words: Mesothelioma; Diagnosis; Meta-analysis; Fibulin-3

Abstract: Phage display technology is a kind of laboratory technique by means of genetic engineering to combine phage with exogenous genes, based on the presentation of a heterogeneous peptide or protein libraries on the surface of phage along with phage coat protein expressions. The displayed proteins or peptides could maintain their own relative spatial structure and biological activity, allowing them to direct phage binding to a target of interest. Therefore, the technology can be widely used as a simple and effective screening tool to screen successfully tumor-targeted peptides, deliver tumor-targeted drugs and prepare tumor antibody, which plays an important role in tumor-targeted therapy. Key words: Phage display; Random peptide library; Neoplasms; Molecular targeted therapy

Abstract: Objective To evaluate the diagnostic values of microRNAs (miRNAs) as plasma biomarkers for early diagnosis of non-small cell lung cancer (NSCLC). Methods The levels of 10 miRNAs in plasma of 59 patients with early stage (stage Ⅰ-ⅢA) NSCLC (lung cancer group) and 59 benign lesions (control group) were detected by real-time quantitative polymerase chain reaction (RT-PCR). The levels of serum cytokeratins antigen 21-1(CYFRA21-1), carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC) and other tumor markers were detected by electrochemiluminescence immunoassay (ECLIA), chemiluminescence immunoassay (CLIA) and immunoradiometric assay (IRMA). The early diagnostic value of miRNAs and other markers were evaluated by receiver-operating-characteristic (ROC) curve analysis. The sensitivity, specificity and area under the curve were calculated for the cut-off value. Results Plasma CYFRA21-1, miR-486 and miR-210 levels were significantly different in lung cancer group and control group (CYFRA21-1: 8.896±3.681 vs. 5.892±2.028, P= 0.020; miR-486:2.778±0.778 vs. 1.746±0.892, P < 0.001; miR-210: 4.836±1.374 vs. 2.829±1.503, P < 0.001). Area under ROC curve of CYFRA 21-1, miR-486 and miR-210 was 0.624 (sensitivity: 0.576, specificity: 0.797), 0.848 (sensitivity: 0.831, specificity: 0.780) and 0.751 (sensitivity: 0.746, specificity: 0.746), respectively. MiR-486, miR-210 combined with CYFRA21-1 had the highest diagnostic efficiency, and the area under the curve was 0.924 (sensitivity: 0.847, specificity: 0.811), miR-486 combined with miR-210 had the highest diagnostic efficiency, and the area under the curve was 0.892 (sensitivity: 0.831, specificity: 0.780). Conclusions MiR-486 and miR-210 could be potential biomarkers for diagnosis of NSCLC. Plasma miRNAs combined with tumor markers can improve the diagnostic efficacy of early stage NSCLC. Key words: Carcinoma, non-small-cell lung; MicroRNAs; Diagnosis