Anatomy Research 

Abstract: ObjectiveTo explore the relationship between genetic polymorphisms in glutathione-S-transferase M1 (GSTM1) and adenocarcinoma of the colorectal MethodsGenomic DNA was isolated from the peripheral lymphocytes of 56 patients with colorectal cancer and 143 age-sex matched control subjects Polymerase chain reaction was used to examine the polymorphisms of GSTM1 genes and the relation between these genotypes and risk of AGC was analyzed ResultsThe frequency of the null GSTM1 genotype in patients showed a no statistically significant increase as compared to that in the controls ConclusionGSTM1 null genotype may not be a genetic susceptibility factor for colorectal cancer

Abstract: Objective To establish a HPLC method to determine the contents of S-Gatifloxacin.Methods The stationary phase was UltimateXB-C18 column; The flow rate was 1.0 mL/min and the temperature of column was 29℃.The mobile phase consisted of methanol-0.08mol/L KH_2PO_4 (40:60) adjusted to pH 3.87 with phosphoric acid.The detection wavelength was 285 nm.20 μL of sample solution was injected to the sampler.Results The calibration curve of S-Gatifloxacin was linear in the range of 10.3- 51.5 μg/mL(r=0.9995) with recovery of 98.0% and RSD of 1.2%(n=6).The retention time was 9.887.Conclusion The method is simple and accurate, and can be used in quality control of the development of S-Gatifloxacin preparation.

Abstract: Objective To investigate the effects of crocetin on proliferation,apoptosis and migration of human MG63 myeloma cells and explore its potential mechanism.Methods MG63 cells were treated with crocetin at different concentrations(0,50,100,200,400 μmol / L) for 48 h.Llight microscope was used to observe the change of morphology;MTT was used for the analysis of cell viability;wound healing was performed to observe the cell migration;Western blot was used to identify the expression of apoptosis-related protein(bcl-2,caspase-3,p53 and p21).Results The result of the microscope presented the typical morphological changes of apoptosis.The result of MTT showed that the cell viability decreased from(100 ±9.5)% to(26.8 ±4)% after adding the concentration of crocetin from 0 to 400 μmol / L,compared with the controlled group with significant difference(P0.01).The result of wound healing showed that the migration behavior was lowered with the increasing concentration of crocetin.The result of immunoreaction suggested that the average gray value of bcl-2 / β-actin were decreased from(56.09 ±4.36)% to(18.78 ±1.20)%,while the average gray value of caspase-3,p53 and p21 were increased from(28.61 ±1.71)%,(13.90±1.65)%,(21.18±1.95)% to(71.37±2.46)%,(112.36±2.97)%,(97.43±3.48)%,respectively,all in a dose-dependent manner,compared with the controlled group with significant difference(P0.01).Conclusion Crocetin not only induced the apoptosis but also inhibited the proliferation of MG63 cells,which may be related to the down-regulation of the expression of bcl-2 gene and the up-regulation of the expression of caspase-3 、 p53 and p21.

Abstract: Objective To explore the preparation method of microsphere,optimize the preparation techniques and prescription compositionBSA was used as model protein,PLGA was used as encapsulation material to prepare microspheresMethods Microspheres were prepared using a(water-in-oil)-in-water double emulsion solvent evaporation techniqueThe particle size of microspheres was measured and the encapsulation efficiency and burst release of the microspheres were calculatcdThe concentration of BSA was determined by micro BCA assayAnd through these,the effects of the concentration of BSA,the amount of PLGA,the concentration of PVA and the power of ultrasonication on the particle size,encapsulation efficiency and burst release of microspheres were studiedResults Through orthogonal test,the preparation techniques of microspheres were optimizedThe optimization parameters are 10 mg for BSA,250 mg for PLGA,15% PVA and 60w for ultrasonicatin powerConclusion Smooth spherical microspheres with relatively high encapsulation efficiency,low burst release amount,proper particle size and proper drug loading amount could be produced by controlling different process parameter