TL;DR: By combining the least complicated and expedient methods of sample handling with the sensitivity and specificity of the GSH assay by enzymatic recycling and the small volumes and software capabilities of microtiter plate technology, this work devised a rapid, sensitive, and easy assay for GSH and GSSG in biological samples.

TL;DR: The advantage of this method versus one-step zero-length crosslinking is that only one component of the complex is exposed to the crosslinker, which eliminates complications arising from the formation of crosslinks among several proteins of a multicomponent complex.

TL;DR: All assays utilize the Cobas FARA clinical automated analyzer and provide considerable time savings over the manual assays.

TL;DR: Evidence is presented to support a proposed mechanism that oxidative formation of these compounds involves the formation of endoperoxide intermediates which are directly reduced by naturally occurring biological substances to PGF2 compounds.

TL;DR: A rapid temperature cycler of low thermal mass was constructed and a 536-bp beta-globin fragment of human genomic DNA was easily visualized with ethidium bromide on agarose gels with rapid cycling.

TL;DR: This method has been successfully applied to the isolation and purification of DNA from eight different adult insects and can be cleaved with restriction endonucleases, ligated efficiently using standard cloning vectors, and hybridized to synthetic oligonucleotides.

TL;DR: A simplified method is described here for isolating the recombinant Taq enzyme after overproduction in Escherichia coli and contains a single, nearly homogeneous protein consistent with the previously established size of the Taq DNA polymerase.

TL;DR: This method will allow the identification of chemical components, receptors, or ionic channels present in a specific type of cell, to determine their relevance to the regulation of the differential secretion of specific materials present in one but not in the other cell type and to ascertain whether the released materials from one cell type affect the functions of the other.

TL;DR: Radioisotopic assays for the determination of acetyl-CoA, CoASH, and acetylcarnitine have been modified for application to the amount of human muscle tissue that can be obtained by needle biopsy.

TL;DR: A spectrophotometric method is described for the determination of sarcoplasmic reticulum (SR) Ca2(+)-ATPase activity (EC 3.1.6.38) in unfractionated muscle homogenates, which shows a good correlation over a wide range between SR specific Ca2 (+)-uptake and -ATPases activities.

TL;DR: The assay is characterized by a sensitivity sufficiently high to detect the various forms of glutathione in plasma, by an analytical recovery of GSH and GSSG close to 100%, and by a within-day precision corresponding to a coefficient of variation of 7%.

TL;DR: Overnight electrophoresis was replaced by short gel runs and overnight capillary transfer by rapid vacuum-blotting adapted to Northern analysis and the duration of the procedure was shortened drastically, allowing an autoradiography signal to be obtained within 24 h.

TL;DR: The length distribution of the glycan strands in the murein (peptidoglycan) sacculus of Escherichia coli has been analyzed after solubilization of the muresin by complete digestion with human serum amidase.

TL;DR: A method where lectins conjugated with digoxigenin are used in combination with an anti-digoxigen in antibody AP conjugate as a very sensitive detection system for carbohydrate structures analysis of blotted glycoproteins is described.

TL;DR: This assay relies on the use of a large excess of a low affinity ligand which removes and complexes all low molecular weight iron and iron nonspecifically bound to serum proteins.

TL;DR: The addition of NaOH to the protein assay reagent reduced the variation in the response of this assay to different proteins, and the sensitivity of the assay is increased.

TL;DR: A new method for the separation of sialic acids at neutral pH on a Carbopac PA-1 anion-exchange column of pellicular resin, with pulsed amperometric detection following postcolumn addition of alkali is reported.

TL;DR: The oligosaccharides in ovalbumin as a glycoprotein model were released with anhydrous hydrazine, and reductively pyridylaminated after re-N-acetylation, and analyzed by capillary zone electrophoresis with on-column fluorometric detection.

TL;DR: The analytical methods described are the most sensitive (10-30 fmol injection-1) and specific (utilizing both excitation and emissions properties and electrochemical reaction potentials, respectively) methods for determining kynurenic acid in brain tissue extracts and CSF.

TL;DR: Ruthenium red provides a simple, rapid method for detecting various types of Ca2(+)-binding proteins following electrophoresis and bound to the same proteins detected by the 45Ca2+ overlay technique.

TL;DR: The ELISA measurement of BrdU incorporation compares favorably with measurements of tritiated thymidine incorporation and offers the additional advantages that the same microculture can be examined for cell number, for cell morphology, and for the percentage of cells having Brd U-labeled nuclei and other antigens.

TL;DR: A capillary system in which protein-surface interactions have been minimized, resulting in high efficiencies (greater than or equal to 300,000 theoretical plates), which allows the analysis of a set of protein standards over a wide pI range at neutral pH and moderate ionic strength is reported.

TL;DR: The present method for preparation of peptides from proteins separated by sodium dodecyl sulfate gel electrophoresis is quite simple and can be used for sequence analysis of proteins in general at the subnanomolar level.

TL;DR: The use of nucleic acid probes directly labeled with horseradish peroxidase for detection of single copy sequences on Southern blots of human genomic DNA by enhanced chemiluminescence is described.

TL;DR: Fast ion bombardment-mass spectrometry was employed to confirm the structures of the various acyl-CoAs and yields were greater than 90% and the purities greater than 95%, based on the distribution of radioactivity, and chromatographic and spectral properties.

TL;DR: The microcomputer-aided determination of cell proliferation kinetics and doubling times utilizing a crystal violet assay and a 3-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay in microtitration plates is described.

TL;DR: Regression equations have been determined which can be used to calculate concentrations of bovine lactoperoxidase (LPO), human salivary peroxidases (SPO), and human myeloperoxIDase (MPO) from activities measured with the following donors: pyrogallol, guaiacol, 2,2'-azinobis, and thiocyanate (SCN-).