Soonchunhyang University

Abstract: The role of epithelial-to-mesenchymal transition (EMT) in metastasis is a longstanding source of debate, largely owing to an inability to monitor transient and reversible EMT phenotypes in vivo. Here we establish an EMT lineage-tracing system to monitor this process in mice, using a mesenchymal-specific Cre-mediated fluorescent marker switch system in spontaneous breast-to-lung metastasis models. We show that within a predominantly epithelial primary tumour, a small proportion of tumour cells undergo EMT. Notably, lung metastases mainly consist of non-EMT tumour cells that maintain their epithelial phenotype. Inhibiting EMT by overexpressing the microRNA miR-200 does not affect lung metastasis development. However, EMT cells significantly contribute to recurrent lung metastasis formation after chemotherapy. These cells survived cyclophosphamide treatment owing to reduced proliferation, apoptotic tolerance and increased expression of chemoresistance-related genes. Overexpression of miR-200 abrogated this resistance. This study suggests the potential of an EMT-targeting strategy, in conjunction with conventional chemotherapies, for breast cancer treatment.

Abstract: A new defected ground structure (DGS) for the microstrip line is proposed in this paper. The proposed DGS unit structure can provide the bandgap characteristic in some frequency bands with only one or more unit lattices. The equivalent circuit for the proposed defected ground unit structure is derived by means of three-dimensional field analysis methods. The equivalent-circuit parameters are extracted by using a simple circuit analysis method. By employing the extracted parameters and circuit analysis theory, the bandgap effect for the provided defected ground unit structure can be explained. By using the derived and extracted equivalent circuit and parameters, the low-pass filters are designed and implemented. The experimental results show excellent agreement with theoretical results and the validity of the modeling method for the proposed defected ground unit structure.