TL;DR: The present experimental study defines two novel inhibitory mechanisms of Ang II formation in the human heart interstitium, which appear to be inhibited by the endogenous inhibitor Ang-(1-9) formed by heart CPA-like activity.
Abstract: Background Data from in vitro studies suggest that both chymase and ACE contribute to the local generation of angiotensin (Ang) II in the heart. The enzyme kinetics under in vivo conditions are unclear. We thus studied the generation of Ang II by cardiac tissue in the presence of interstitial fluid (IF) that contains a variety of naturally occurring protease inhibitors.
Methods and Results Ang I was incubated with heart homogenate in the presence of IF. IF obtained from human skin contained substantial amounts of protease inhibitors and ACE activity, the concentration of α1-antitrypsin being 35% and the activity of ACE 24% of the corresponding serum values. When heart homogenate was incubated with Ang I, three enzymes were responsible for its metabolism: heart chymase and heart ACE converted Ang I to Ang II, and heart carboxypeptidase A (CPA)–like activity degraded Ang I to Ang-(1-9). Incubation of heart homogenate in the presence of IF led to practically full inhibition of heart chymase–mediated Ang II formation by the natural protease inhibitors present in IF. In contrast, heart CPA–like activity was not blocked, as reflected by the continued generation of Ang-(1-9). In addition, both heart ACE– and IF ACE–mediated Ang II formation were strongly inhibited. This inhibition was shown to be due to the Ang-(1-9) formed.
Conclusions The present experimental study defines two novel inhibitory mechanisms of Ang II formation in the human heart interstitium. Heart chymase–mediated Ang II formation is strongly inhibited by the natural protease inhibitors present in the IF. Similarly, both heart ACE– and IF ACE–mediated Ang II formation appear to be inhibited by the endogenous inhibitor Ang-(1-9) formed by heart CPA–like activity. These inhibitory mechanisms provide additional information about how the Ang II concentration in the heart interstitium may be controlled.
TL;DR: The species of ophiostomatoid fungi associated with I. typographus japonicus in Japan are almost identical to those associated with the beetles infesting Norway spruce in Europe and this study improves the knowledge of the biogeography of the ophiobacterial fungi and the insects with which they are associated.
TL;DR: Isolates of the large tree type (LTT) Gremmeniella from North America, Italy and Iceland contained RAMS alleles not observed in Finland, Sweden or Norway, therefore, the isolates of LTT Gremmaniella should not be transported even within the area of its natural occurrence.
TL;DR: The fractile cut-points used to define abnormal lipid values had a profound influence on the diagnosis of FCHL, and no single ultracentrifugation variable could discriminate reliably affected family members from non-affected family members.
TL;DR: The seedling development of two Southeast Asian dipterocarp trees in response to differences in irradiance and spectral quality and the growth of this taxon may be constrained by adaptations, particularly in leaves, for drought tolerance.
Abstract: Seedling developmental responses to understory shade combine the effects of reductions in irradiance and changes in spectral quality. We studied the seedling development of two Southeast Asian dipterocarp trees in response to differences in irradiance (photosynthetic photon flux density, PPFD) and spectral quality (red to far-red ratio, R:FR). The two species, Hopea helferei and H. odorata, are taxonomically closely related but differ in their ecological requirements; H. helferei is more drought-tolerant and typically grows in more open habitats. Seedlings were grown in six different replicated shadehouse treatments varying in percentage of solar PPFD and R:FR. The two species differed in the influence of light variables on most seedling characters, particularly for final height, internode distance, branch/trunk internodes, stem length/mass, leaf area/stem length, petiole length, and growth/mol of photons received. Most of the characters in both taxa were primarily influenced by PPFD, but spectral quality also influenced some characters - more so for H. odorata. The latter species grew more rapidly, particularly in the low PPFD treatments, and its leaves were capable of higher photosynthesis rates. However, growth in H. helferei was not reduced in direct sunlight. The growth of this taxon may be constrained by adaptations, particularly in leaves, for drought tolerance.
TL;DR: It is argued that, when mixing two habitat types A and B, the ratio of these habitats that maximize avian diversity depends on the ratios of species confined to habitat A andB, as well as the number of species favoured by the mixture of A and A.
TL;DR: Goats may be better adapted than sheep to subsist on browse in kermes oak shrublands, but high levels of herbage that result from shrub management allow sheep to utilize this type of forage.
TL;DR: In this article, the internal mechanical stress components in trees when exposed to bending and torsion by wind forces were derived for trees with a rectangular crown and the stem taper so that the maximum bending stress is equal at all heights.
Abstract: Expressions are derived for the internal mechanical stress components in trees when exposed to bending and torsion by wind forces. The crown is modeled as rectangular, and the stem taper so that the maximum bending stress is equal at all heights. Trees with asymmetric crowns will undergo torsion as well as bending, and the shear stresses associated with torsion are determined and compared to the bending stresses. The results show that shear failure is likely to occur relatively high up on the stem when the crown asymmetry is a few decimeters. The fact that torsion might be critical to the strength of trees towards wind forces, introduces an explanation to spiral grain growth. It is shown that spiral grain in the direction of the wind-induced torque improves the bending strength of the tree. Spiral grain is therefore beneficial for trees with a prevailing external torque.
TL;DR: In this article, the build-up of greenhouse gases in the atmosphere can be seen as a depletion of a non-renewable resource, namely the absorptive capacity of the atmosphere.
Abstract: The build‐up of greenhouse‐gases (GHG's) in the atmosphere can be seen as a depletion of a non‐renewable resource, namely the absorptive capacity of the atmosphere. A tax scheme on fossil fuels cou...
TL;DR: Functional assays of the graft specimens showed an increased UPA/TPA ratio and a decreased total fibrinolytic activity in comparison with normal veins, which may contribute to the graft occlusion.
Abstract: Background Intimal smooth muscle cell proliferation is an underlying pathogenetic mechanism for neointimal hyperplasia and consequent vein graft failure. This study characterizes the expression of tissue-type plasminogen activator (TPA), urokinase-type plasminogen activator (UPA), and plasminogen activator inhibitor-1 (PAI-1) in hyperplastic vein grafts and normal venous tissue. Methods and Results Failing graft and control vein specimens from 14 donors were homogenized, and TPA and PAI-1 were quantified with ELISA. The amount of PAI-1 was seven times higher (4.2±2.1 versus 0.6±0.6 ng/mg protein, P<.005), but the TPA antigen content was markedly lower (3.1±2.1 versus 8.1±3.7 ng/mg protein, P<.005) in the stenosed grafts compared with the control veins. Strong immunohistochemical PAI-1 reactivity and in situ hybridization signals for PAI-1 and UPA mRNA were associated with the smooth muscle cells of the thickened intima of the grafts. Functional assays of the graft specimens showed an increased UPA/TPA rat...
TL;DR: Findings imply that activation of vascular mast cells with ensuing secretion of HEP-PGs may locally attenuate the thrombogenicity of matrix collagen by inhibiting its platelet-activating capacity.
Abstract: Mast cells, the major source of tissue heparin, line the vascular system. On stimulation, rat serosal mast cells release soluble heparin proteoglycans (HEP-PGs) of very high molecular weight (750 000). We compared the effects of HEP-PGs and standard heparins (average molecular weights, 15 000 and 5 000) on platelet-collagen interactions in vitro. In contrast with the standard heparins, HEP-PGs completely inhibited collagen-induced platelet aggregation and serotonin release in platelet-rich plasma. The inhibition caused by HEP-PGs depended on its macromolecular structure. In flowing blood, HEP-PGs also inhibited platelet deposition on a collagen-coated surface both at low and high shear rates. Although HEP-PGs did not block glycoprotein (GP) Ia/IIa-mediated platelet adhesion, they attenuated subsequent platelet activation and aggregation, as well as fibrinogen binding to platelets after collagen stimulation. HEP-PGs did not bind to platelets but bound tightly to von Willebrand factor (vWf) and enhanced its binding to collagen. Although platelet adhesion at high shear rate and vWf binding to GP Ib after ristocetin stimulation were not markedly affected, HEP-PGs reduced thrombin-induced aggregation and vWf binding to GP IIb/IIIa. These findings imply that activation of vascular mast cells with ensuing secretion of HEP-PGs may locally attenuate the thrombogenicity of matrix collagen by inhibiting its platelet-activating capacity.
TL;DR: This is the first conclusive study indicating that in resin acid enriched sediments, anaerobic processes form resin acid derived base neutral compounds.
TL;DR: The Geochemical Atlas of Slovak Republic (49,036 km 2 ) at a scale of 1 : 1,000,000 was compiled during 1991-1995 together with maps of associated geochemical and ecological features.
TL;DR: In this article, the cyclic structure of cyclic block designs is used to obtain designs balanced for order effects and nearly balanced for carry-over effects, and a computer intensive discrete optimisation method which can be used when a cyclic design is not possible.
TL;DR: The aboveground biomass built up annually by Allium ursinum (L.) contains similar amounts of nutrients as the foliage of mature Fagus sylvatica (L.), and substantial amounts of nitrogen are lost from the system.
Abstract: The aboveground biomass built up annually by Allium ursinum (L.) contains similar amounts of nutrients as the foliage of mature Fagus sylvatica (L.) stands. The decomposition of the A. ursinum stand in early summer provides N rich forage for grazing mesofauna, especially favouring collembolans and accelerating mineralization of soil organic matter. Short term decreases of soil pH had no negative effect on populations of collembolans. Synergistic effects from soil fauna and microbes may accelerate nitrogen release from decomposing leaf litter. A positive feed back may have emerged. High animal abundance and diverse mesofauna populations are capable of high rates of litter fragmentation. Consequently, favourable conditions for microorganisms are created and allow high rates of mineralization and release of nutrients. Our data show that substantial amounts of nitrogen are lost from the system. Undisturbed forest ecosystems are considered to recycle mineralized nitrogen efficiently though. But temporal uncoupling of the N cycle due to microbial activity and delayed or decreased N uptake of higher plants can cause enhanced leaching even from undisturbed systems. The tendency to loose nutrients is apparent from high nitrate concentrations in the soil solution throughout the year. When nutrient losses from A. ursinum subsystems are considered, lateral nutrient imports from adjacent parts of the ecosystem have to be taken into account. If lateral import does not counterbalance losses, maintenance of the soil nutrient status must occur by weathering or a decline is to be expected.
TL;DR: A whole catchment liming experiment was conducted at Tjonnstrond, southernmost Norway, to test the utility of terrestrial liming as a technique to restore fish populations in remote lakes with short water-retention times as discussed by the authors.
Abstract: In June 1983 a whole-catchment liming experiment was conducted at Tjonnstrond, southernmost Norway, to test the utility of terrestrial liming as a technique to restore fish populations in remote lakes with short water-retention times. Tjonnstrond consists of 2 small ponds of 3.0 and 1.5 ha in area which drain a 25-ha catchment. The area is located at about 650–700 meters above sea-level in sparse and unproductive forests of spruce, pine and birch with abundant peatlands. A dose of 3 ton/ha of powdered limestone were spread by helicopter to the terrestrial area. No limestone was added to the ponds themselves. The ponds were subsequently stocked with brown and brook trout.
TL;DR: In this paper, the effects of kiln schedule temperature, wood moisture content and kiln relative humidity on kiln brown stain formation were investigated, and the results showed that kiln Brown stain was greatly reduced by using kiln schedules of less than 50°C as opposed to 90°C and was also more pronounced in the kilning at 30% kiln humidity, than at 80% humidity.
Abstract: Kiln drying of radiata pine (Pinus radiata D. Don) sapwood induces a brown discolouration, commonly called kiln brown stain. Kiln brown stain generally occurs 0.5–1.5 mm under the surface of the dry sawn lumber. However, subsequent planing of the wood can expose the stain causing substantial revenue loss in the New Zealand highvalue export markets. In the present study, the effects of kiln schedule temperature, wood moisture content and kiln relative humidity on kiln brown stain formation were investigated. The results showed that kiln brown stain was greatly reduced by using kiln schedules of less than 50°C as opposed to 90°C and was also more pronounced in the kilning at 30% kiln relative humidity, than at 80% humidity. Furthermore, the occurrence of kiln brown stain tended to intensify with decreasing wood moisture content, irrespective of kiln temperature and kiln humidity used. The practical implication of this work and the mechanisms of kiln brown stain formation are discussed.
TL;DR: Examination of seven-year series of crown-condition data from Local County Monitoring Plots in Norway spruce stands in Norway gave no evident support for the hypothesized negative effect on crown condition from sulphur and nitrogen deposition and related alterations in soil.
TL;DR: It was found that annual biomass was statistically different among species in all the study years with Robinia being the most productive of all the species tested followed by the other two legumes Colutea and Amorpha.
Abstract: Productivity and its relation to air temperature and precipitation of 11 deciduous woody fodder species was studied in a semi-arid Mediterranean environment with cold winters in Macedonia, Greece. The species studied included six shrubs: Amorpha fruticosa L., Carpinus orientalis Mill, Colutea arborescens L., Corylus avellana L., Fraxinus ornus L. and Ostrya carpinifolia Scop.; and four trees: Pirus amygdaliformis Vill., Quercus pubescens Wild., Quercus sessiliflora Salish. and Robinia pseudoacacia L., with two accessions, common and spineless. Fodder species were established as one-year-old seedlings in 1987 and studied through 1994. Productivity was measured by clearcutting of the aboveground annual biomass (DM) at the end of each growing season. Samples of biomass were hand separated to determine the grazeable material (leaves and twigs). In addition, monthly values of air temperature and precipitation were correlated with annual biomass through a multiple linear regression analysis. It was found that annual biomass was statistically different among species in all the study years with Robinia being the most productive of all the species tested followed by the other two legumes Colutea and Amorpha. Proportion of grazeable material ranged widely among species and years with an overall mean of 50%. Robinia produced grazeable biomass more than 1000 kg/ha followed by Amorpha and Colutea with about 800 and 700 kg DM/ha, respectively, while all the other species produced less than 500 kg DM/ha. Yearly variation in annual biomass was found to be affected much more by air temperature, especially during the beginning of the growing season (March), than by precipitation. Coefficients of determination (r2) were found to be high (0.50–0.80) in Amorpha, Pirus, Quercus and Robinia but only in the latter two species they were high enough (>0.70) so that the relations produced can be used for predictive purposes.
TL;DR: In this article, a multi-layered composite building material is provided which allows effective utilization of forest resources by using so-called low quality materials such as small-diameter wood, old wood, pieces of wood produced as byproducts of lumbering, and/or bamboo.
Abstract: A multi-layered composite building material is provided which allows effective utilization of forest resources by using so-called low quality materials such as small-diameter wood, old wood, pieces of wood produced as byproducts of lumbering, and/or bamboo. The material can meet various requirements and properties, and can be produced at a low cost. In a structural layer, an adhesive agent is applied to a plurality of finely split pieces which are formed by finely splitting a raw material such as wood or bamboo. The finely split pieces are arranging in parallel to a fiber direction. A shock/vibration-absorbing layer is formed by applying an adhesive agent to small pieces of wood and bamboo and the like. Structural layers and shock/vibration-absorbing layers are alternately arranged to provide a multi-layered structure. The structure is press-molded to a predetermined thickness and optionally heated such that the layers are adhered together to produce the composite multi-layered material. The shock/vibration-absorbing layer may be mixed with resin formed, for example, as a pellet shape, to provide a mixed vibration-absorbing material with improved properties.
TL;DR: Results indicate that mean leaf size, and the incidence of species with vertical leaf inclination are significantly correlated with site productivity, and of the PFAs assessed, five elements appear to offer a useful basis for grouping species for modelling.
TL;DR: In this paper, a mathematical model for high-temperature drying of softwood lumber with moist air has been modified and extended to simulate wood drying with superheated steam, and the predicted drying curves and wood temperatures were compared with experimental data and there was close agreement.
Abstract: A mathematical model for high-temperature drying of softwood lumber with moist air has been modified and extended to simulate wood drying with superheated steam. In the simulation, differences between the two types of drying are considered, these include: external heat and mass transfer processes and calculation of equilibrium moisture content. The external mass transfer coefficient in the perheated steam drying was found to be much higher than that in the moist air drying, however, the heat ransfer coefficients for these two cases were of the same order. The predicted drying curves and wood temperatures from the superheated steam drying model were compared with experimental data and there was close agreement. Further studies will apply the model to development of commercial drying schedules for wood drying with superheated steam.
TL;DR: Following a decrease in photosynthetic capacity in July, the cumulative budget became negative and the branches died, indicating that a negative carbon budget corresponds with the onset of abscission of the lowest branches.
Abstract: A cost-benefit approach was developed to analyze the carbon budget of the lowest Scots pine (Pinus sylvestris L.) branches subject to abscission. In addition to within-branch growth and respiratory costs, the budget included an estimation of a branch's share of the maintenance respiration of the stem and root. A branch was considered productive if the budget was positive. Foliar gas exchange and woody-tissue respiration were non-destructively measured at monthly intervals during the growing season on the six lowest branches of 10-year-old Scots pine trees, to the moment when the branches died naturally. Photosynthetic light response and temperature response of respiration, together with measurements of canopy light conditions and meteorological data, were used to calculate seasonal carbon budgets for the branches. Maintenance respiration of stems and roots was estimated from published data. All but one of the branches studied were found to be nonproductive over the growing season. Following a decrease in photosynthetic capacity in July, the cumulative budget became negative and the branches died, indicating that a negative carbon budget corresponds with the onset of abscission of the lowest branches.
TL;DR: Exocytosed heparin-bound chymase may be involved in the development of both early (fatty streaks) and late (thrombotic) atherosclerotic lesions.
Abstract: Many of the mast cells present in human atherosclerotic lesions contain chymase. As the lesions progress to more severe forms, the number of such mast cells increases, and their activity (degree of degranulation) increases. Exocytosed heparin-bound chymase may be involved in the development of both early (fatty streaks) and late (thrombotic) atherosclerotic lesions. Experimental studies with rat serosal mast cells have revealed that chymase can degrade the apolipoprotein B-100 component of low-density lipoprotein (LDL) particles and the apolipoprotein A component of high-density lipoprotein (HDL) particles. Both of these chymase actions on apolipoproteins tend to increase the cholesterol content of macrophages and to convert them into the foam cells typical of early atherosclerotic lesions. In atheromatous plaques, the late atherosclerotic lesions, the chymase-containing mast cells may render the plaques unstable and their caps susceptible to rupture when chymase activates the interstitial procollagenase secreted by the macrophages in the plaque caps. Definition of the quantitative importance of chymase in the pathogenesis of atherosclerosis and its complications remains an exciting challenge for the future.
TL;DR: The fern Dryopteris filix-mas was exceptional, containing on average three times greater fluoride concentrations man rowan, and high background levels indicated that soil uptake contributed significantly to the fluoride accumulation in this species.
Abstract: Summary
The accumulation of fluoride in natural vegetation exposed to emissions from five aluminium smelter plants in Norway was studied during the years 1990–93. About 2000 leaf, bark and twig samples of 60 plant species, collected mostly during the growing season, were analysed. Rowan (Sorbus aucuparia) was widespread and common in the areas studied, and was used as a reference species. Fluoride concentrations in monthly samples of rowan leaves were linearly related to fluoride exposure (average fluoride concentration in ambient air × days since leaf emergence). The accumulation coefficient for rowan was estimated to be 1.7 m3/g dry wt. day. Most other species had values between 0.3 and 1.5; the median for all species was 0.8. The fern Dryopteris filix-mas was exceptional, containing on average three times greater fluoride concentrations man rowan. High background levels indicated that soil uptake contributed significantly to the fluoride accumulation in this species. The fluoride concentrations in bark and shoots of trees were mostly low compared with leaves, but the bark of Betula pendula and B. pubescens had very high concentrations.
TL;DR: Comparisons with existing edging and trimming procedures show that substantial reductions in solution time can be achieved, and some procedures for determining the optimal cutting of flitches into graded dimensional boards are described.
Abstract: Log breakdown can be viewed as a two-stage process with logs sawn into slabs of wood known as flitches during the primary stage and flitches further processed during the secondary stage to produce edged (cut lengthwise) and trimmed (cut widthwise) pieces. This paper addresses the secondary problem and describes some procedures for determining the optimal cutting of flitches into graded dimensional boards. The problem is formulated as a set packing problem with the objective being to maximise total value. Extensions to the basic formulation include constraints which restrict the number of saws and which disallow waste between adjacent edged pieces. The problem is solved using dynamic programming techniques and the algorithms incorporated into a sawing simulation system. Comparisons with existing edging and trimming procedures show that substantial reductions in solution time (to as little as 1/25th of the time required for an enumerative search) can be achieved.
TL;DR: An increase in plasma LDL influx into the skin at sites where mast cells are stimulated is demonstrated, and so suggests that mast cells play a role in the accumulation of LDL cholesterol that occurs in the skin when xanthomas form.
TL;DR: The distribution of bacteria into protein-pattern groups differed significantly between the two samples, suggesting variation in culturable bacterial flora.
Abstract: The cultivated and uncultivated bacterial communities of an activated sludge plant were studied. Two samples were taken and a total of 516 bacterial isolates were classified into groups using their whole-cell protein patterns. The distribution of bacteria into protein-pattern groups differed significantly between the two samples, suggesting variation in culturable bacterial flora. Partial 16S rRNA gene sequences were determined for representatives of the commonest protein-pattern groups. Most of the sequences obtained were previously unknown, but relatively closely related to known sequences of organisms belonging to the α, β or γ subclasses of the proteobacteria, the first two subclasses being predominant. This classification of bacteria isolated on a diluted nutrient-rich medium differed from recent culture-dependent studies using nutrient-rich media. The uncultivated bacterial community was studied by analyzing ten partial 16S rRNA gene sequences cloned directly from activated sludge. None of the cloned sequences was identical to those determined for culturable organisms; or to those in the GenBank database. They were, however, related to the α or β subclasses of the proteobacteria, or to the gram-positive bacteria with a high G+C DNA content.
TL;DR: Foliage of oriental hornbeam and manna ash have the potential to be an effective source of protein for small ruminants during the dry Mediterranean summer.